PD-L1 Antibody (2096C) [Unconjugated]

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and J774A.1 mouse reticulum cell sarcoma macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Mouse PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB90781) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PD-L1/B7-H1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

RAW 264.7 mouse monocyte/macrophage cell line either treated with LPS overnight (filled histogram) or untreated (open histogram) was stained with Rabbit Anti-Mouse PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB90781), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

HEK293 human embryonic kidney cell line transfected with either (A) mouse PD-L1/B7-H1 or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Mouse PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB90781) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

PD-L1/B7-H1 was detected in perfusion fixed frozen sections of mouse thymus using Rabbit Anti-Mouse PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB90781) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to thymocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Mu
• Applications: WB, Flow, IHC
• Clone: 2096C
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

PD-L1 Antibody (2096C) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse PD-L1/B7-H1
  Met1-His239
  Accession # Q9EP73
• Specificity: Detects mouse PD-L1/B7-H1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PD-L1 Antibody (2096C) [Unconjugated]

• Avelumab
• B7-H
• B7H1
• B7-H1
• B7H1PDCD1L1
• CD274 antigenMGC142294
• CD274 molecule
• CD274
• PDCD1L1
• PDCD1LG1
• PDCD1LG1MGC142296
• PDL1
• PD-L1
• PD-L1B7 homolog 1
• PDL1PDCD1 ligand 1
• programmed cell death 1 ligand 1
• Programmed death ligand 1

Background

Mouse B7 homolog 1(B7-H1), also called programmed death ligand 1 (PD-L1) and programmed cell death 1 ligand 1 (PDCD1L1), is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1-4). Other family members include B7-1, B7-2, B7-H2, B7-H3 and PD-L2. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and a short cytoplasmic region. Among the family members, they share from 20-40% amino acid (aa) sequence identity. The cloned mouse B7-H1/PD-L1 cDNA encodes a 290 aa type I membrane precursor protein with a putative 18 aa signal peptide, a 220 aa extracellular region containing one V-like and one C-like Ig domain, a 22 aa transmembrane region, and a 30 aa cytoplasmic domain. Mouse and human B7-H1/PD-L1 share approximately 70% aa sequence identity. B7-H1/PD-L1 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immunoreceptors. The other identified ligand is PD-L2. Mouse B7-H1/PD-L1 and PD-L2 share approximately 34% aa sequence identity and have similar functions. B7-H1/PD-L1 is constitutively expressed in various lymphoid and non-lymphoid organs including placenta, heart, pancreas, lung, liver, and endothelium
(1‑4). The expression of B7-H1/PD-L1 is detected on B cells, T cells, monocytes, dendritic cells and thymic epithelial cells. IFN-gamma treatment induces B7-H1/PD-L1 expression in monocytes, dendritic cells, and endothelial cells. B7-H1/PD-L1 expression is also upregulated in a variety of tumor cell lines. On previously activated T cells, B7-H1/PD-L1 interaction with PD-1 inhibits TCR-mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. In contrast, a costimulatory function for the PD-1 ligands on resting T cells has also beenreported (1-4).

1. Tamura, H. et al. (2001) Blood 97:1809.
2. Freeman, G. et al. (2000) J. Exp. Med. 192:1027.
3. Sharpe, A.H. and G. J. Freeman (2002) Nat. Rev. Immunol. 2:116.
4. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PD-L1 Antibody (MAB90781)(12)

Publications using MAB90781

• Cao, Y;Ye, Q;Ma, M;She, QB; Enhanced bypass of PD-L1 translation reduces the therapeutic response to mTOR kinase inhibitors Cell reports 2023-07-04 [PMID: 37405918] (Western Blot, Mouse)
• Shrestha, M;Wang, DY;Ben-David, Y;Zacksenhaus, E; CDK4/6 inhibitors and the pRB-E2F1 axis suppress PVR and PD-L1 expression in triple-negative breast cancer Oncogenesis 2023-05-26 [PMID: 37230983] (Western Blot, Mouse)
• S Zhang, X You, T Xu, Q Chen, H Li, L Dou, Y Sun, X Xiong, MA Meredith, Y Sun PD-L1 induction via the MEK-JNK-AP1 axis by a neddylation inhibitor promotes cancer-associated immunosuppression Cell Death & Disease, 2022-10-03;13(10):844. 2022-10-03 [PMID: 36192389] (Neutralization, Mouse)
• Joseph B. Hiatt, Holly Sandborg, Sarah M. Garrison, Henry U. Arnold, Sheng-You Liao, Justin P. Norton et al. Inhibition of LSD1 with Bomedemstat Sensitizes Small Cell Lung Cancer to Immune Checkpoint Blockade and T-Cell Killing Clinical Cancer Research 10/14/2022 [PMID: 35920742]
• X Wang, K Wang, M Yu, D Velluto, X Hong, B Wang, A Chiu, JM Melero-Mar, AA Tomei, M Ma Engineered immunomodulatory accessory cells improve experimental allogeneic islet transplantation without immunosuppression Science Advances, 2022-07-22;8(29):eabn0071. 2022-07-22 [PMID: 35867788] (ICC, Mouse)
• K Skibbe, AK Brethack, A Sünderhauf, M Ragab, A Raschdorf, M Hicken, H Schlichtin, J Preira, J Brandt, D Castven, B Föh, R Pagel, JU Marquardt, C Sina, S Derer Colorectal Cancer Progression Is Potently Reduced by a Glucose-Free, High-Protein Diet: Comparison to Anti-EGFR Therapy Cancers, 2021-11-19;13(22):. 2021-11-19 [PMID: 34830971] (Western Blot, Mouse)
• W Xu, J Cui, L Wu, C He, G Chen The role of PD-1/PD-L1 checkpoint in arsenic lung tumorigenesis Toxicology and Applied Pharmacology, 2021-06-22;426(0):115633. 2021-06-22 [PMID: 34166680] (IHC, Mouse)
• L Gao, L Xia, W Ji, Y Zhang, W Xia, S Lu Knockdown of CDK5 down-regulates PD-L1 via the ubiquitination-proteasome pathway and improves antitumor immunity in lung adenocarcinoma Translational Oncology, 2021-06-12;14(9):101148. 2021-06-12 [PMID: 34130052] (Western Blot, Mouse)
• Ling X, Han W, Jiang X, et al. Intestinal TMEM16A control luminal chloride secretion in a NHERF1 dependent manner Biomaterials 1/1/2021 [PMID: 33561626]
• P Yang, Y Jiang, PR Rhea, T Coway, D Chen, M Gagea, SL Harribance, L Cohen Human Biofield Therapy and the Growth of Mouse Lung Carcinoma Integr Cancer Ther, 2019-01-01;18(0):1534735419840. 2019-01-01 [PMID: 30947564] (Western Blot, Mouse)
• W Deng, S Zhu, L Zeng, J Liu, R Kang, M Yang, L Cao, H Wang, TR Billiar, J Jiang, M Xie, D Tang The Circadian Clock Controls Immune Checkpoint Pathway in Sepsis Cell Rep, 2018-07-10;24(2):366-378. 2018-07-10 [PMID: 29996098] (Western Blot, Mouse)
• Jinfang Zhang, Xia Bu, Haizhen Wang, Yasheng Zhu, Yan Geng, Naoe Taira Nihira et al. Cyclin D–CDK4 kinase destabilizes PD-L1 via cullin 3–SPOP to control cancer immune surveillance Nature 1/4/2018 [PMID: 29160310]

Reviews for PD-L1 Antibody (MAB90781) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-P Mouse 04/03/2020

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Breast cancer tissue
• Species: Mouse
• Lot: CKKJ011803B

reviewed by: Emily Ehlerding ICC Mouse 05/16/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Mouse Lymph Node
• Species: Mouse

Bioinformatics

• Uniprot:
  • Q9EP73()