PD-L1 Antibody (2096C) [Unconjugated]

Images

 
RAW 264.7 mouse monocyte/macrophage cell line either treated with LPS overnight (filled histogram) or untreated (open histogram) was stained with Rabbit Anti-Mouse PD-L1/B7-H1 Monoclonal Antibody (Catalog # ...read more
HEK293 human embryonic kidney cell line transfected with either (A) mouse PD-L1/B7-H1 or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Mouse PD-L1/B7-H1 Monoclonal Antibody (Catalog # ...read more
Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and J774A.1 mouse reticulum cell sarcoma macrophage cell line untreated (-) or treated (+) with 10 μg/mL LPS for 4 hours. PVDF ...read more
PD-L1/B7-H1 was detected in perfusion fixed frozen sections of mouse thymus using Rabbit Anti-Mouse PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB90781) at 5 µg/mL for 1 hour at room temperature followed by ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC
Clone
2096C
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

PD-L1 Antibody (2096C) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PD-L1/B7-H1
Met1-His239
Accession # Q9EP73
Specificity
Detects mouse PD-L1/B7-H1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-25 ug/mL
Control
PD-L1 Knockout A549 Cell Lysate
Reviewed Applications
Read 2 Reviews rated 4.5
using
MAB90781 in the following applications:

Publications
Read Publications using
MAB90781 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PD-L1 Antibody (2096C) [Unconjugated]

  • Avelumab
  • B7-H
  • B7H1
  • B7-H1
  • B7H1PDCD1L1
  • CD274 antigenMGC142294
  • CD274 molecule
  • CD274
  • PDCD1L1
  • PDCD1LG1
  • PDCD1LG1MGC142296
  • PDL1
  • PD-L1
  • PD-L1B7 homolog 1
  • PDL1PDCD1 ligand 1
  • programmed cell death 1 ligand 1
  • Programmed death ligand 1

Background

Mouse B7 homolog 1(B7-H1), also called programmed death ligand 1 (PD-L1) and programmed cell death 1 ligand 1 (PDCD1L1), is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1-4). Other family members include B7-1, B7-2, B7-H2, B7-H3 and PD-L2. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and a short cytoplasmic region. Among the family members, they share from 20-40% amino acid (aa) sequence identity. The cloned mouse B7-H1/PD-L1 cDNA encodes a 290 aa type I membrane precursor protein with a putative 18 aa signal peptide, a 220 aa extracellular region containing one V-like and one C-like Ig domain, a 22 aa transmembrane region, and a 30 aa cytoplasmic domain. Mouse and human B7-H1/PD-L1 share approximately 70% aa sequence identity. B7-H1/PD-L1 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immunoreceptors. The other identified ligand is PD-L2. Mouse B7-H1/PD-L1 and PD-L2 share approximately 34% aa sequence identity and have similar functions. B7-H1/PD-L1 is constitutively expressed in various lymphoid and non-lymphoid organs including placenta, heart, pancreas, lung, liver, and endothelium
(1‑4). The expression of B7-H1/PD-L1 is detected on B cells, T cells, monocytes, dendritic cells and thymic epithelial cells. IFN-gamma treatment induces B7-H1/PD-L1 expression in monocytes, dendritic cells, and endothelial cells. B7-H1/PD-L1 expression is also upregulated in a variety of tumor cell lines. On previously activated T cells, B7-H1/PD-L1 interaction with PD-1 inhibits TCR-mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. In contrast, a costimulatory function for the PD-1 ligands on resting T cells has also beenreported (1-4).

  1. Tamura, H. et al. (2001) Blood 97:1809.
  2. Freeman, G. et al. (2000) J. Exp. Med. 192:1027.
  3. Sharpe, A.H. and G. J. Freeman (2002) Nat. Rev. Immunol. 2:116.
  4. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PD-L1 Antibody (MAB90781)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Western Blot
(2)
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Filter By Species
Mouse
(2)
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Reviews for PD-L1 Antibody (MAB90781) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using MAB90781:
Filter by Applications
IHC-P
(1)
ICC
(1)
All Applications
Filter by Species
Mouse
(2)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin PD-L1 MAB90781
Enlarge
5
reviewed by:
Anonymous
IHC-P Mouse 04/03/2020
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Tested breast cancer tissue
SpeciesMouse
LotCKKJ011803B
Immunocytochemistry PD-L1 MAB90781
Enlarge
4
reviewed by:
Emily Ehlerding
ICC Mouse 05/16/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedMouse Lymph Node
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PD-L1 Antibody (MAB90781) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB90781U
Alexa Fluor 405 FAB90781V
Alexa Fluor 488 FAB90781G
Alexa Fluor 594 FAB90781T
Alexa Fluor 647 FAB90781R
Alexa Fluor 700 FAB90781N
Alexa Fluor 750 FAB90781S

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
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Anonymous
04/03/2020
Application: IHC-P
Species: Mouse

Emily Ehlerding
05/16/2017
Application: ICC
Species: Mouse

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