PD-L2/B7-DC/PDCD1LG2 Antibody (176611) [Unconjugated]

Images

 
PD‑L2/B7‑DC was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human PD‑L2/B7‑DC Monoclonal Antibody (Catalog # MAB1224) at 0.5 µg/mL for 1 hour at ...read more
HepG2 human hepatocellular carcinoma cell line was stained with Human PD‑L2 Monoclonal Antibody (Catalog # MAB1224, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clone
176611
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

PD-L2/B7-DC/PDCD1LG2 Antibody (176611) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human PD-L2/B7-DC
Leu20-Pro219 (predicted)
Accession # Q9BQ51
Specificity
Detects human PD-L2/B7-DC in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse PD-L2, recombinant human (rh) B7-1, rhB7-2, rhB7-H1, rhB7-H2, or rhB7-H3 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PDCD1LG2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 0.5-25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB1224 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PD-L2/B7-DC/PDCD1LG2 Antibody (176611) [Unconjugated]

  • B7-DC
  • bA574F11.2
  • Btdc
  • Butyrophilin B7-DC
  • Butyrophilin-like Protein
  • CD273 antigen
  • CD273
  • CD273PD-1 ligand 2
  • MGC142240
  • PD-1-ligand 2
  • PDCD1L2MGC142238
  • PDCD1LG2
  • PDL2
  • PD-L2
  • PDL2B7DC
  • PD-L2PDCD1 ligand 2
  • programmed cell death 1 ligand 2
  • Programmed death ligand 2

Background

T cells require a signal induced by the engagement of the T cell receptor and a “co‑stimulatory” signal(s) through distinct T cell surface molecules for optimal T cell activation and tolerance. Members of the B7 superfamily of counter-receptors were identified by their ability to interact with co‑stimulatory molecules found on the surface of T cells. Members of the B7 superfamily include B7-1 (CD80), B7-2 (CD86), B7-H1 (PD-L1), B7-H2 (B7RP-1), B7-H3, and PD-L2 (B7-DC) (1). B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and short cytoplasmic domains. Among the family members, they share from 20‑40% amino acid (aa) sequence identity. The cloned human PD-L2 cDNA encodes a 273 aa type I membrane precursor protein with a putative 20 aa signal peptide, a 201 aa extracellular region containing one V-like and one C-like Ig domain, a 24 aa transmembrane region, and a 28 aa cytoplasmic domain. The extracellular domains of mouse and human PD-L2 share approximately 70% aa sequence identity (2). PD-L2 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immuno-receptors. The other identified ligand is PD-L1. Human PD-L1 and PD-L2 share approximately 41% aa sequence identity and have similar functions. PD-L2 is broadly expressed in tissues. Highest expression was detected by Northern blot analysis in heart, placenta, liver, pancreas, spleen, and lymph node. Lower amounts of expression were observed in lung, smooth muscle, and thymus. Expression of PD-L2 on antigen presenting cell has been examined in detail. Resting B cells, monocytes and dendritic cells do not express PD-L2, expression however can be induced by LPS or BCR activation in B cells, INF-gamma treatment in monocytes, or LPS plus IFN-gamma treatment of dendritic cells. PD-L2 expression is also up regulated in a variety of tumor cell lines. On previously activated T cells, PD-L2 interaction with PD-1 inhibits TCR-mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. In contrast, a co‑stimulatory function for the PD-L2 on resting T cells activated with sub-optimal TCR signals has also been reported (3).

 

  1. Coyle, A.J. and J-C. Gutierrrez-Ramos (2001) Nature Immunol. 2:203.
  2. Latchman Y. et al. (2001) Nature Immun. 2:261.
  3. Carreno, B.M. and M. Collins (2002) Annu. Rev. Immunol. 20:29.
 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-76769
Species: Hu, Mu, Rt
Applications: Dual ISH-IHC, ELISA, Flow, ICC/IF, IF, IHC-WhMt, IHC, IHC-Fr, IHC-P, IP, KD, WB
AF1086
Species: Hu
Applications: Block, CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, IHC, WB
MAB140
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, IHC, Neut
MAB7158
Species: Hu, Mu
Applications: IHC, WB
7268-CT
Species: Hu
Applications: BA
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
NBP2-25208
Species: Hu, Mu, Rt
Applications: Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, KO, WB
NBP1-19371
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
202-IL
Species: Hu
Applications: BA
NBP1-49045
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
DY417
Species: Mu
Applications: ELISA
MAB342
Species: Hu
Applications: AgAct, WB
AF165
Species: Hu
Applications: CyTOF-ready, Flow, WB
6507-IL/CF
Species: Hu
Applications: BA
NBP1-72042
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
DDX0131P-100
Species: Hu
Applications: Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P
AF169
Species: Hu
Applications: IHC, WB

Publications for PD-L2/B7-DC/PDCD1LG2 Antibody (MAB1224)(13)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Flow Cytometry, ICC, IHC, IHC-P, Western Blot.


Filter By Application
Flow Cytometry
(3)
ICC
(2)
IHC
(2)
IHC-P
(4)
Western Blot
(2)
All Applications
Filter By Species
Human
(11)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using MAB1224 Applications Species
MA Murayama, E Takada, K Takai, N Arimitsu, J Shimizu, T Suzuki, N Suzuki Nicotine treatment regulates PD-L1 and PD-L2 expression via inhibition of Akt pathway in HER2-type breast cancer cells PLoS ONE, 2022;17(1):e0260838. 2022 [PMID: 35085258] (ICC, Human) ICC Human
A Makowska, N Lelabi, C Nothbaum, L Shen, P Busson, TTB Tran, M Eble, U Kontny Radiotherapy Combined with PD-1 Inhibition Increases NK Cell Cytotoxicity towards Nasopharyngeal Carcinoma Cells Cells, 2021;10(9):. 2021 [PMID: 34572108] (Flow Cytometry, Human) Flow Cytometry Human
T Ono, K Azuma, A Kawahara, T Kakuma, F Sato, T Kawaguchi, J Akiba, H Umeno Changes in immune parameters between pre-treatment and recurrence after (chemo) radiation therapy in patients with head and neck cancer Sci Rep, 2020;10(1):11973. 2020 [PMID: 32686757] (IHC, Human) IHC Human
HK Ahn, S Kim, D Kwon, J Koh, YA Kim, K Kim, DH Chung, YK Jeon MET Receptor Tyrosine Kinase Regulates the Expression of Co-Stimulatory and Co-Inhibitory Molecules in Tumor Cells and Contributes to PD-L1-Mediated Suppression of Immune Cell Function Int J Mol Sci, 2019;20(17):. 2019 [PMID: 31480591] (Western Blot, Human) Western Blot Human
J Lin, J Long, X Wan, J Chen, Y Bai, A Wang, X Yang, Y Wu, SC Robson, X Sang, H Zhao Classification of gallbladder cancer by assessment of CD8+ TIL and PD-L1 expression BMC Cancer, 2018;18(1):766. 2018 [PMID: 30055582] (IHC-P, Human) IHC-P Human
ME Mostafa, A Abdelkader, N Kuroda, D Pérez-Mont, A Banerjee, O Hes, KA Iczkowski Variation in nuclear size and PD-L2 positivity correlate with aggressive chromophobe renal cell carcinoma Ann Diagn Pathol, 2018;34(0):31-35. 2018 [PMID: 29661724] (IHC-P, Human) IHC-P Human
A Lamarca, D Nonaka, W Breitwiese, G Ashton, J Barriuso, MG McNamara, S Moghadam, J Rogan, W Mansoor, RA Hubner, C Clark, B Chakrabart, JW Valle PD-L1 expression and presence of TILs in small intestinal neuroendocrine tumours Oncotarget, 2018;9(19):14922-14938. 2018 [PMID: 29599916] (ICC, Human) ICC Human
MA Lakins, E Ghorani, H Munir, CP Martins, JD Shields Cancer-associated fibroblasts induce antigen-specific deletion of CD8+T Cells to protect tumour cells Nat Commun, 2018;9(1):948. 2018 [PMID: 29507342] (Flow Cytometry, Mouse) Flow Cytometry Mouse
H Chang, JH Hong, JK Lee, HW Cho, YT Ouh, KJ Min, KA So Programmed death-1 (PD-1) expression in cervical intraepithelial neoplasia and its relationship with recurrence after conization J Gynecol Oncol, 2018;0(0):. 2018 [PMID: 29400020] (IHC-P, Human) IHC-P Human
L Cao, X Wang, S Li, Q Zhi, Y Wang, L Wang, K Li, R Jiang PD-L1 is a Prognostic Biomarker in Resected NSCLC Patients with Moderate/high Smoking History and Elevated Serum SCCA Level J Cancer, 2017;8(16):3251-3260. 2017 [PMID: 29158797] (IHC-P, Human) IHC-P Human
Show All 13 Publications.

Reviews for PD-L2/B7-DC/PDCD1LG2 Antibody (MAB1224) (0)

There are no reviews for PD-L2/B7-DC/PDCD1LG2 Antibody (MAB1224). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PD-L2/B7-DC/PDCD1LG2 Antibody (MAB1224) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional PD-L2/B7-DC/PDCD1LG2 Products

Bioinformatics Tool for PD-L2/B7-DC/PDCD1LG2 Antibody (MAB1224)

Discover related pathways, diseases and genes to PD-L2/B7-DC/PDCD1LG2 Antibody (MAB1224). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PD-L2/B7-DC/PDCD1LG2 Antibody (MAB1224)

Discover more about diseases related to PD-L2/B7-DC/PDCD1LG2 Antibody (MAB1224).
 

Pathways for PD-L2/B7-DC/PDCD1LG2 Antibody (MAB1224)

View related products by pathway.

PTMs for PD-L2/B7-DC/PDCD1LG2 Antibody (MAB1224)

Learn more about PTMs related to PD-L2/B7-DC/PDCD1LG2 Antibody (MAB1224).

Blogs on PD-L2/B7-DC/PDCD1LG2

There are no specific blogs for PD-L2/B7-DC/PDCD1LG2, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PD-L2/B7-DC/PDCD1LG2 Antibody (176611) [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol PDCD1LG2
Uniprot