![Immunohistochemistry-Paraffin: PD-1 Antibody (SPM597) - Azide and BSA Free [NBP2-47806] - Human Tonsil stained with PD1 (CD279) Monoclonal Antibody (SPM597).](http://images.novusbio.com/fullsize2/PD-1 Antibody (SPM597) - Azide and BSA Free-Immunohistochemistry-Paraffin-NBP2-47806-img0001.jpg "Immunohistochemistry-Paraffin: PD-1 Antibody (SPM597) - Azide and BSA Free [NBP2-47806] - Human Tonsil stained with PD1 (CD279) Monoclonal Antibody (SPM597).")
| Reactivity | HuSpecies Glossary |
| Applications | Flow, IHC, IHC-P, CyTOF-ready, ICC/IF |
| Clone | SPM597 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Recombinant full-length human PDCD1 protein |
| Localization | Cell Surface & Cytoplasmic |
| Specificity | PDCD-1 (programmed cell death-1 protein), also designated CD279, is a type I transmembrane receptor and a member of the immunoglobin gene superfamily. It is expressed on activated T-cells, B-cells, and myeloid cells. Anti-PDCD-1 is a marker of angioimmunoblastic lymphoma and suggests a unique cell of origin for this neoplasm. Unlike CD10 and BCL6, PDCD-1 is expressed by few B-cells, so anti-PDCD-1 may be a more specific and useful diagnostic marker in angioimmunoblastic lymphoma. In addition, PDCD-1 expression provides evidence that angioimmunoblastic lymphoma is a neoplasm derived from germinal center-associated T-cells. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PDCD1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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| Theoretical MW | 55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-47806AF350 | |
| Alexa Fluor 405 | NBP2-47806AF405 | |
| Alexa Fluor 488 | NBP2-47806AF488 | |
| Alexa Fluor 532 | NBP2-47806AF532 | |
| Alexa Fluor 594 | NBP2-47806AF594 | |
| Alexa Fluor 647 | NBP2-47806AF647 | |
| Alexa Fluor 700 | NBP2-47806AF700 | |
| Alexa Fluor 750 | NBP2-47806AF750 | |
| Allophycocyanin | NBP2-47806APC | |
| Allophycocyanin/Cy7 | NBP2-47806APCCY7 | |
| Biotin | NBP2-47806B | |
| DyLight 350 | NBP2-47806UV | |
| DyLight 405 | NBP2-47806V | |
| DyLight 488 | NBP2-47806G | |
| DyLight 550 | NBP2-47806R | |
| DyLight 594 | NBP2-47806DL594 | |
| DyLight 650 | NBP2-47806C | |
| DyLight 680 | NBP2-47806FR | |
| DyLight 755 | NBP2-47806IR | |
| FITC | NBP2-47806F | |
| HRP | NBP2-47806H | |
| Janelia Fluor 549 | NBP2-47806JF549 | |
| Janelia Fluor 646 | NBP2-47806JF646 | |
| PE | NBP2-47806PE | |
| PE/Atto594 | NBP2-47806PEATT594 | |
| PE/Cy5.5 | NBP2-47806PECY55 | |
| PE/Cy7 | NBP2-47806PECY7 | |
| PerCP | NBP2-47806PCP |
Diseases for PD-1 Antibody (NBP2-47806)Discover more about diseases related to PD-1 Antibody (NBP2-47806).
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PTMs for PD-1 Antibody (NBP2-47806)Learn more about PTMs related to PD-1 Antibody (NBP2-47806).
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| Gene Symbol | PDCD1 |
![Knockdown Validated: PD-L1 Antibody [NBP1-76769] - Validation with PD-L1 siRNA Knockdown. HeLa cells were transfected with control siRNAs (lane 1) or PD-L1 siRNAs (lane 2). Loading: 10 ug of HeLa whole cell lysates per lane. Antibodies: NBP1-76769 (2 ug/mL) and GAPDH (0.02 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/images2/PD-L1-Antibody-Knockdown-Validated-NBP1-76769-img0024.jpg)
![Western Blot: PD-L1 Antibody [NBP1-76769] - Independent Antibody Validation (IAV) via Protein Expression ProfileLoading: 15 ug of lysates per lane.Antibodies: NBP1-76769 (2 ug/mL), PD-L1 (2 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit and or anti-mouse IgG HRP conjugate at 1:10000 and 1:5000 dilution, respectively.](http://images.novusbio.com/images2/PD-L1-Antibody-Western-Blot-NBP1-76769-img0023.jpg)
![Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP1-76769] - CD274 (PD-L1) expression in the HNSCC patients from the TCGA database, HNSCC tissue samples and the HNSCC cells. Immunostaining of PD-L1 obtained from HNSCC tumor cells, immune cells and tumor margin tissues in HNSCC tissue samples (magnification A-200, scale bars 50AI1/4m) : low tumor staining; moderate tumor staining; high tumor staining have been observed. Image collected and cropped by Cite Ab from the following publication (Lactoferricin B reverses cisplatin resistance in head and neck squamous cell carcinoma cells through targeting PD-L1. Cancer Med (2018)) licensed under a CC-BY licence.](http://images.novusbio.com/images2/PD-L1-Antibody-Immunohistochemistry-Paraffin-(Negative)-NBP1-76769-img0025.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0018.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/images2/HLA DQ Antibody (SPV-L3)-Flow Cytometry-NBP2-45041-img0001.jpg)
![Western Blot: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Analysis in human Raji lymphoma cells.](http://images.novusbio.com/images2/B7-1-CD80-Antibody-(62N3G8)-Western-Blot-NBP2-25255-img0010.jpg)
![Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Human thymus stained with CD80 antibody at 5 ug/ml using peroxidase-conjugate and DAB chromogen. Note membrane and cytoplasmic staining.](http://images.novusbio.com/images2/B7-1-CD80-Antibody-(62N3G8)-Immunohistochemistry-Paraffin-NBP2-25255-img0002.jpg)
![Flow Cytometry: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Raji lymphoma cells. Image from verified customer review.](http://images.novusbio.com/images2/B7-1-CD80-Antibody-(62N3G8)-Flow-Cytometry-NBP2-25255-img0009.jpg)
![Immunohistochemistry: CD4 Antibody [NBP1-19371] - Increased CD3+ and CD4+ T-cell occurrence in the brainstem of SHR-72 transgenic rat model for tauopathies. (A-D) Immunofluorescence staining showed CD4+ T-cells in SHR-72 transgenic animals. (E- H) Immunofluorescence staining showed more perivascular than brain parenchyma infiltrating CD4+ T-cells in SHR-72 transgenic animals. PLoS One. 2019 May 23;14(5):e0217216. doi: 10.1371/journal.pone.0217216.](http://images.novusbio.com/images2/CD4-Antibody-Immunohistochemistry-NBP1-19371-img0017.jpg)
![Immunohistochemistry: CD4 Antibody [NBP1-19371] - DBZ inhibits the accumulation of CD4+ T cells and Th2 differentiation in the AAAs. (B) The representation of immunohistochemical staining for CD4+ in abdominal aorta from four groups (left). Bar graphs show the percentage of CD4+ positive cell areas (right; n=3 per group). Bar: 50 um.](http://images.novusbio.com/images2/CD4-Antibody-Immunohistochemistry-NBP1-19371-img0018.jpg)
![Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.](http://images.novusbio.com/images2/CD4-Antibody-Western-Blot-NBP1-19371-img0002.jpg)
![Flow Cytometry: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by multiple staining.](http://images.novusbio.com/images2/CD8-Antibody-(53-6.7)-Flow-Cytometry-NBP1-49045-img0014.jpg)
![Immunohistochemistry-Paraffin: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - CD8 alpha expression in mouse spleen tissue using anti-CD8 alpha antibody. Image from verified customer review.](http://images.novusbio.com/images2/CD8-Antibody-(53-6.7)-Immunohistochemistry-Paraffin-NBP1-49045-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of bone marrow tissue by single and multiple staining.](http://images.novusbio.com/images2/CD8-Antibody-(53-6.7)-Immunocytochemistry-Immunofluorescence-NBP1-49045-img0006.jpg)
![Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Mouse spleen stained with FoxP3 antibody. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/images2/FoxP3-Antibody-Immunohistochemistry-Paraffin-NB100-39002-img0011.jpg)
![Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Number of Granzyme B+ and FoxP3+ cells infiltrating tumors. Representative images of IHC staining of FoxP3+ and Granzyme B+ cells in the peri-tumoral areas in two mice characterized by low FoxP3+ and high Granzyme B+ (panels 1 and 2) or high FoxP3+ and low Granzyme B+ (panels 3 and 4). Image collected and cropped by Cite Ab from the following publication (Inhibition of tumor growth by cancer vaccine combined with metronomic chemotherapy and anti-PD-1 in a pre-clinical setting. Oncotarget (2018) licensed under a CC-BY licence.](http://images.novusbio.com/images2/FoxP3-Antibody-Immunohistochemistry-Paraffin-NB100-39002-img0025.jpg)
![Immunohistochemistry: FoxP3 Antibody [NB100-39002] - CCR10-expressing CD4+CD25+FOXP3+ Treg cells are enriched in the spinal cord after SCI. Double immunostaining of FOXP3 (red) and CCR10 (green) in the spinal cord sections after sham or SCI surgery. The merged images are also shown. Arrows indicate cells with positive staining and colocalization. Scale bar, 100 i1/4m. Data are mean A+- SD. Image collected and cropped by Cite Ab from the following publication (CCL28 promotes locomotor recovery after spinal cord injury via recruiting regulatory T cells. Aging (Albany NY) (2019) licensed under a CC-BY licence.](http://images.novusbio.com/images2/FoxP3-Antibody-Immunohistochemistry-NB100-39002-img0027.jpg)
![Western Blot: ISG20 Antibody (1B2-3C9) [H00003669-M01] - Analysis of ISG20 expression in transfected 293T cell line by ISG20 monoclonal antibody (M01), clone 1B2-3C9.Lane 1: ISG20 transfected lysate(20.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/ISG20-Antibody-(1B2-3C9)-Western-Blot-H00003669-M01-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ISG20 Antibody (1B2-3C9) [H00003669-M01] - Analysis of monoclonal antibody to ISG20 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/images2/ISG20-Antibody-(1B2-3C9)-Immunocytochemistry-Immunofluorescence-H00003669-M01-img0006.jpg)
![Sandwich ELISA: ISG20 Antibody (1B2-3C9) [H00003669-M01] - Detection limit for recombinant GST tagged ISG20 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/images2/ISG20-Antibody-(1B2-3C9)-Sandwich-ELISA-H00003669-M01-img0007.jpg)
![Knockout Validated: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Western blot shows lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2 knockout (KO) Ramos cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human B7-2 Monoclonal Antibody (Catalog # NBP2-25208) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for B7-2 at approximately 90 kDa (as indicated) in the parental Ramos cell line, but is not detectable in the knockout Ramos cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/images2/B7-2-CD86-Antibody-(BU63)-Knockout-Validated-NBP2-25208-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Immunofluorescence staining of PFA-fixed Ramos cells using B7-2/CD86 Antibody (BU63) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.](http://images.novusbio.com/images2/B7-2-CD86-Antibody-(BU63)-Immunocytochemistry-Immunofluorescence-NBP2-25208-img0006.jpg)
![Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Formalin-fixed, paraffin-embedded esophagus tumor tissue stained with CD86 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Note strong staining of differentiated squamous cells. TMA was used for this test.](http://images.novusbio.com/images2/B7-2-CD86-Antibody-(BU63)-Immunohistochemistry-Paraffin-NBP2-25208-img0004.jpg)
![IL-4 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "IL-4 [Unconjugated]")
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Involucrin signal in human keratinocytes. Red: anti-IVL antibody. BLACK: Isotype control NBP1-43319APC at 1:100. Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/images2/Mouse IgG1 Kappa Light Chain Isotype Control (P3.6.2.8.1)-Flow Cytometry-NBP1-43319-img0004.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Involucrin signal in human keratinocytes. Red: anti-IVL antibody. BLACK: Isotype control NBP1-43319APC at 1:100. Flow cytometry image submitted by a verified customer review.")