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PD-1 Antibody Pair [HRP]

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Sandwich ELISA: PD-1 Antibody Pair [HRP] [NBP2-79528] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Sandwich ELISA: PD-1 Antibody Pair [HRP] [NBP2-79528] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, S-ELISA
Clonality
Monoclonal
Host
Mouse
Conjugate
HRP
Standard Curve Range
3.91-250 pg/ml (example only; lot dependent)
Sensitivity
3.91 pg/ml (example only; lot dependent)

Order Details

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PD-1 Antibody Pair [HRP] Summary

Description
Solid Phase sandwich ELISA for the quantitative determination of Human PD-1.
Specificity
This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human PD-1.
Standard Curve Range
3.91-250 pg/ml (example only; lot dependent)
Sensitivity
3.91 pg/ml (example only; lot dependent)
Assay Type
Sandwich ELISA
Sample Volume
100 ul
Clonality
Monoclonal
Host
Mouse
Gene
PDCD1

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA
Application Notes
1. Capture Antibody: Dilute to a working concentration of 2 ug/ml in PBS before coating.
2. Detection Antibody: Dilute to working concentration of 0.3 ug/ml in Dilution Buffer before use.
3. Standard: Reconstitute with 1 ml Dilution Buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 250 pg/ml is recommended.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Capture Antibody: 1 mg/ml of mouse anti-Human PD-1 monoclonal antibody (in PBS, pH 7.4).
  2. Detection Antibody: 0.2 mg/ml of mouse anti-Human PD-1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  3. Standard: Each vial contains 57 ng of recombinant Human PD-1.

Alternate Names for PD-1 Antibody Pair [HRP]

  • CD279 antigen
  • CD279
  • hPD-1
  • PD1
  • PD-1
  • PD1hPD-l
  • PDCD1
  • programmed cell death 1
  • programmed cell death protein 1
  • Protein PD-1
  • SLEB2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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  Manual - PD-1 Antibody Pair [HRP]
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Bioinformatics

Gene Symbol PDCD1