PD-1 Antibody (J43)

Images

 
Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Analysis of PE conjugate of NBP1-43110. The J43 antibody has been tested by flow cytometric mouse Con-A activated spleen cell suspensions and mouse PD-1 transfected ...read more
Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.25 ug of Hamster IgG Isotype Control Purified (open histogram) or 0.25 ...read more
Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Analysis using the Biotin conjugate of NBP1-43110. Staining of ConA stimulated splenocytes with Anti-Mouse CD279 (PD-1) FITC (left) and PE (right). Appropriate isotype ...read more
Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Analysis using the Serum/Azide Free version of NBP1-43110. Staining of mouse Con-A activated spleen cell suspensions and mouse PD-1 transfected cells using PD1 PE ...read more
Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Analysis using the Serum/Azide Free version of NBP1-43110. Staining of unstimulated (orange histogram) or 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC-Fr, IP
Clone
J43
Clonality
Monoclonal
Host
Armenian Hamster
Concentration
0.5 mg/ml

PD-1 Antibody (J43) Summary

Immunogen
The immunogen for this antibody was PD1.
Isotype
IgG
Clonality
Monoclonal
Host
Armenian Hamster
Gene
PDCD1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Conjugate
Unconjugated
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
The J43 antibody has been tested by flow cytometric analysis of mouse Con-A activated spleen cell suspensions and mouse PD-1 transfected cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.IHC-F reactivity reported in scientific literature (PMID: 12847137 )

Alternate Names for PD-1 Antibody (J43)

  • CD279 antigen
  • CD279
  • hPD-1
  • PD-1
  • PD1hPD-l
  • PDCD1
  • programmed cell death 1
  • programmed cell death protein 1
  • Protein PD-1
  • SLEB2

Background

The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. It is reported that J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells. Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-524
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP, Flow-IC
NBP1-84550
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, InhibTFunc
MAB342
Species: Hu
Applications: WB, Flow, AgAct
MAB8214
Species: Hu, Mu
Applications: Flow, IHC, ICC
H00003669-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP1-43110
Species: Mu
Applications: Flow, IHC-Fr, IP

Publications for PD-1 Antibody (NBP1-43110) (0)

There are no publications for PD-1 Antibody (NBP1-43110).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PD-1 Antibody (NBP1-43110) (0)

There are no reviews for PD-1 Antibody (NBP1-43110). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for PD-1 Antibody (NBP1-43110). (Showing 1 - 1 of 1 FAQ).

  1. Can you tell me if this antibody is azide free, carrier free, with an endotoxin rate known and smaller than 10EU/mg?
    • I can tell you that this antibody contains 0.09% sodium azide (i.e. is not azide free). I can now inform you that none of our current lots of NBP1-43110 contain carrier protein, but this antibody is not endotoxin tested. I hope that NBP1-43110 meets your requirements.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

PE Labeled NBP1-43700

Additional PD-1 Antibody (J43) Products

Related Products by Gene

Bioinformatics Tool for PD-1 Antibody (NBP1-43110)

Discover related pathways, diseases and genes to PD-1 Antibody (NBP1-43110). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PD-1 Antibody (NBP1-43110)

Discover more about diseases related to PD-1 Antibody (NBP1-43110).
 

Pathways for PD-1 Antibody (NBP1-43110)

View related products by pathway.

PTMs for PD-1 Antibody (NBP1-43110)

Learn more about PTMs related to PD-1 Antibody (NBP1-43110).
 

Research Areas for PD-1 Antibody (NBP1-43110)

Find related products by research area.

Blogs on PD-1

There are no specific blogs for PD-1, but you can read our latest blog posts.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol PDCD1
Entrez
Uniprot

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP1-43110 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought

PD-1 Antibody (J43) [PE]
NBP1-43700-0.1mg