PD-1 Antibody (J43)

Images

 
Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Analysis of PE conjugate of NBP1-43110. The J43 antibody has been tested by flow cytometric mouse Con-A activated spleen cell suspensions and mouse PD-1 transfected ...read more
Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.25 ug of Hamster IgG Isotype Control Purified (open histogram) or 0.25 ...read more
Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Analysis using the Biotin conjugate of NBP1-43110. Staining of ConA stimulated splenocytes with Anti-Mouse CD279 (PD-1) FITC (left) and PE (right). Appropriate isotype ...read more
Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Analysis using the Serum/Azide Free version of NBP1-43110. Staining of mouse Con-A activated spleen cell suspensions and mouse PD-1 transfected cells using PD1 PE ...read more
Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Analysis using the Serum/Azide Free version of NBP1-43110. Staining of unstimulated (orange histogram) or 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC-Fr, IP
Clone
J43
Clonality
Monoclonal
Host
Armenian Hamster
Conjugate
Unconjugated
Concentration
0.5 mg/ml

PD-1 Antibody (J43) Summary

Immunogen
The immunogen for this antibody was PD1.
Isotype
IgG
Clonality
Monoclonal
Host
Armenian Hamster
Gene
PDCD1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
The J43 antibody has been tested by flow cytometric analysis of mouse Con-A activated spleen cell suspensions and mouse PD-1 transfected cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.IHC-F reactivity reported in scientific literature (PMID: 12847137 )

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for PD-1 Antibody (J43)

  • CD279 antigen
  • CD279
  • hPD-1
  • PD-1
  • PD1hPD-l
  • PDCD1
  • programmed cell death 1
  • programmed cell death protein 1
  • Protein PD-1
  • SLEB2

Background

The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. It is reported that J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells. Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-76769
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NB100-524
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP, Flow-IC
NBP1-84550
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-76770
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, ICC
AF740
Species: Mu
Applications: WB, Flow, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, Neut, ELISA(Sta)
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
MAB342
Species: Hu
Applications: WB, Flow, AgAct, CyTOF-ready
NBP2-12499
Species: Hu
Applications: IHC, IHC-P
NB100-39002
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
H00003669-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
AF2365
Species: Hu
Applications: WB, Flow, CyTOF-ready

Publications for PD-1 Antibody (NBP1-43110) (0)

There are no publications for PD-1 Antibody (NBP1-43110).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PD-1 Antibody (NBP1-43110) (0)

There are no reviews for PD-1 Antibody (NBP1-43110). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for PD-1 Antibody (NBP1-43110). (Showing 1 - 1 of 1 FAQ).

  1. Can you tell me if this antibody is azide free, carrier free, with an endotoxin rate known and smaller than 10EU/mg?
    • I can tell you that this antibody contains 0.09% sodium azide (i.e. is not azide free). I can now inform you that none of our current lots of NBP1-43110 contain carrier protein, but this antibody is not endotoxin tested. I hope that NBP1-43110 meets your requirements.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

PE Labeled NBP1-43700

Additional PD-1 Antibody (J43) Products

Related Products by Gene

Bioinformatics Tool for PD-1 Antibody (NBP1-43110)

Discover related pathways, diseases and genes to PD-1 Antibody (NBP1-43110). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PD-1 Antibody (NBP1-43110)

Discover more about diseases related to PD-1 Antibody (NBP1-43110).
 

Pathways for PD-1 Antibody (NBP1-43110)

View related products by pathway.

PTMs for PD-1 Antibody (NBP1-43110)

Learn more about PTMs related to PD-1 Antibody (NBP1-43110).
 

Research Areas for PD-1 Antibody (NBP1-43110)

Find related products by research area.

Blogs on PD-1.

Thomson Reuters Predicts 2016 Nobel Prize Winners
Here at Bio-Techne we always look forward to the annual announcements of winners of the highly coveted Nobel Prize – the greatest award in science. How can you go about predicting which scientists might be in line for a life-changing phone-call fro...  Read full blog post.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PD-1 Antibody (J43) and receive a gift card or discount.

Bioinformatics

Gene Symbol PDCD1
Entrez
Uniprot

Customers Who Bought This Also Bought

PD-1 Antibody (J43) [PE]
NBP1-43700-0.1mg