Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Analysis of PE conjugate of NBP1-43110. The J43 antibody has been tested by flow cytometric mouse Con-A activated spleen cell suspensions and mouse PD-1 transfected ...read more
Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.25 ug of Hamster IgG Isotype Control Purified (open histogram) or 0.25 ...read more
Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Analysis using the Biotin conjugate of NBP1-43110. Staining of ConA stimulated splenocytes with Anti-Mouse CD279 (PD-1) FITC (left) and PE (right). Appropriate isotype ...read more
Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Analysis using the Serum/Azide Free version of NBP1-43110. Staining of mouse Con-A activated spleen cell suspensions and mouse PD-1 transfected cells using PD1 PE ...read more
Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Analysis using the Serum/Azide Free version of NBP1-43110. Staining of unstimulated (orange histogram) or 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade ...read more
The J43 antibody has been tested by flow cytometric analysis of mouse Con-A activated spleen cell suspensions and mouse PD-1 transfected cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.IHC-F reactivity reported in scientific literature (PMID: 12847137 )
Read Publication using NBP1-43110 in the following applications:
The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. It is reported that J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells. Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for PD-1 Antibody (NBP1-43110). (Showing 1 - 1 of 1 FAQ).
Can you tell me if this antibody is azide free, carrier free, with an endotoxin rate known and smaller than 10EU/mg?
I can tell you that this antibody contains 0.09% sodium azide (i.e. is not azide free). I can now inform you that none of our current lots of NBP1-43110 contain carrier protein, but this antibody is not endotoxin tested. I hope that NBP1-43110 meets your requirements.
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