Home » PD-1 » PD-1 Antibodies » PD-1 Antibody (J43) [HRP]

PD-1 Antibody (J43) [HRP]

 Submit a Review

Images

 
There are currently no images for PD-1 Antibody (NBP1-43110H).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications IHC, IHC-Fr
Clone
J43
Clonality
Monoclonal
Host
Armenian Hamster
Conjugate
HRP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-43110AF350
Alexa Fluor 405
NBP1-43110AF405
Alexa Fluor 488
NBP1-43110AF488
Alexa Fluor 532
NBP1-43110AF532
Alexa Fluor 594
NBP1-43110AF594
Alexa Fluor 647
NBP1-43110AF647
Alexa Fluor 700
NBP1-43110AF700
Alexa Fluor 750
NBP1-43110AF750
Allophycocyanin
NBP1-43110APC
Allophycocyanin/Cy7
NBP1-43110APCCY7
Biotin
NBP1-43110B
DyLight 350
NBP1-43110UV
DyLight 405
NBP1-43110V
DyLight 488
NBP1-43110G
DyLight 550
NBP1-43110R
DyLight 594
NBP1-43110DL594
DyLight 650
NBP1-43110C
DyLight 680
NBP1-43110FR
DyLight 755
NBP1-43110IR
FITC
NBP1-43110F
Janelia Fluor 549
NBP1-43110JF549
Janelia Fluor 646
NBP1-43110JF646
PE
NBP1-43700-0.1mg
PE
NBP1-43110PE
PE
NBP1-43700-0.05mg
PE/Atto594
NBP1-43110PEATT594
PE/Cy5.5
NBP1-43110PECY55
PE/Cy7
NBP1-43110PECY7
PerCP
NBP1-43110PCP
Unconjugated
NBP1-43110-0.5mg
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-43110-0.5mg
Azide Free
NBP2-34497
Azide Free
NBP2-34497-0.025mg
100% Guarantee on every product

PD-1 Antibody (J43) [HRP] Summary

Immunogen
The immunogen for this antibody was PD1.
Isotype
IgG
Clonality
Monoclonal
Host
Armenian Hamster
Gene
PDCD1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for PD-1 Antibody (J43) [HRP]

  • CD279 antigen
  • CD279
  • hPD-1
  • PD1
  • PD-1
  • PD1hPD-l
  • PDCD1
  • programmed cell death 1
  • programmed cell death protein 1
  • Protein PD-1
  • SLEB2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

CTLA-4
[Unconjugated]
7268-CT
Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) inhibits IL-2 secretion by stimulated Jurkat human acute Tcell leukemia cells. The ED50 for this effect is 0.03-0.15 μg/mL whenstimulated with 1 μg/mL Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1).
(3 Reviews)
(1 Publication)
Species: Hu
PD-L1 Antibody
NBP1-76769
Western Blot: PD-L1 Antibody [NBP1-76769] - Upregulated expression of PDAEL1 in cisplatinresistant cells through ILAE6/STAT3. Image collected and cropped by Cite Ab from the following publication (Lactoferricin B reverses cisplatin resistance in head and neck squamous cell carcinoma cells through targeting PD-L1. Cancer Med (2018)) licensed under a CC-BY licence.
Immunohistochemistry-Paraffin (Negative): PD-L1 Antibody [NBP1-76769] - CD274 (PDAEL1) expression in the HNSCC patients from the TCGA database, HNSCC tissue samples and the HNSCC cells. Immunostaining of PDAEL1 obtained from HNSCC tumor cells, immune cells and tumor margin tissues in HNSCC tissue samples (magnification A-200, scale bars 50AI1/4m) : low tumor staining; moderate tumor staining; high tumor staining have been observed. Image collected and cropped by Cite Ab from the following publication (Lactoferricin B reverses cisplatin resistance in head and neck squamous cell carcinoma cells through targeting PD-L1. Cancer Med (2018)) licensed under a CC-BY licence.
Flow Cytometry: PD-L1 Antibody [NBP1-76769] - B7-H1/PD-L1/CD274 Antibody [NBP1-76769] - Analysis of A-20 cells using B7-H1/PD-L1/CD274 antibody at 0.5 ug/mL. Green: Isotype control. Red : B7-H1/PD-L1/CD274 antibody.
(5 Reviews)
(16 Publications)
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, IHC, IHC-Fr, IHC-P, IP, IF, IHC-P (-), KD
NOD2 Antibody (2D9)
NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
(1 Review)
(20 Publications)
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
HLA DQ Antibody
(SPV-L3)
NBP2-45041
Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
(1 Publication)
Species: Hu, Po
Applications: Flow, IHC, IHC-Fr, IF

PD-L2/B7-DC/PDCD1LG2 A
NBP1-76770
Western Blot: PD-L2/B7-DC/PDCD1LG2 Antibody [NBP1-76770] - Loading: 15 ug of lysates per lane. Antibodies: PD-L2 (4 ug/mL), competitor antibody (4 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunocytochemistry/Immunofluorescence: PD-L2/B7-DC/PDCD1LG2 Antibody [NBP1-76770] - analysis of 4% paraformaldehydefixed mouse brain cells labeling PD-L2 with this antibody at 20ug/mL, followed by goat anti-rabbit IgG secondaryantibody at 1/500 dilution (red).
Immunohistochemistry: PD-L2/B7-DC/PDCD1LG2 Antibody [NBP1-76770] - Staining of mouse brain tissue with antibody at 2.5 ug/ml.
(1 Review)
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IF
B7-1/CD80 Antibody
(62N3G8)
NBP2-25255
Western Blot: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Analysis in human Raji lymphoma cells.
Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Human thymus stained with CD80 antibody at 5 ug/ml using peroxidase-conjugate and DAB chromogen. Note membrane and cytoplasmic staining.
Flow Cytometry: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Raji lymphoma cells. Image from verified customer review.
(2 Reviews)
(2 Publications)
Species: Hu, Mu
Applications: WB, Flow, IHC, IHC-P
CD4 Antibody
NBP1-19371
Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.
Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.
(8 Reviews)
(29 Publications)
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
CD8 Antibody
(53-6.7)
NBP1-49045
Immunocytochemistry/Immunofluorescence: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of immersion fixed splenocytes. Primary antibody was used at a dilution of 10 ug/mL and incubated for 3 hours at room temperature.
Immunohistochemistry: CD8 Antibody (53-6.7) [NBP1-49045] - Histopathological analysis of spinal cord in EAE mice. Representative spinal cord sections from wild-type (WT), Asic1-/-, and Asic2-/- mice at 45 days after immunization. Sections of the spinal cord anterior horn were stained with hematoxylin and eosin (E&H) to assess inflammation and immunostained for MCHII, CD4+, CD8+ infiltrating cells. Image collected and cropped by CiteAb from the following publication (https://onlinelibrary.wiley.com/doi/abs/10.1111/ejn.14302), licensed under a CC-BY licence.
Flow Cytometry: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by multiple staining.
(2 Reviews)
(16 Publications)
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
IL-2 [Unconjugated]
202-IL
1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Recombinant Human IL-2 (Catalog # 202-IL) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED50 for this effect is 0.05-0.25 ng/mL.
(22 Reviews)
(302 Publications)
Species: Hu
IL-10 [Biotin]
DY417
(6 Reviews)
(186 Publications)
Species: Mu
CD28 Antibody
(37407) [Unconjugated
MAB342
Human CD28 Monoclonal Antibody enhances IL-2 secretion in the Jurkat human acute T cell leukemia cell line stimulated with 10 ng/mL phorbol myristate acetate (PMA) and 0.5 μM calcium ionophore, in a dose-dependent manner, as measured using the Quantikine Human IL-2 ELISA Kit (Catalog # D2050). The ED50 for this effect is typically 0.2-0.6 μg/mL.
(30 Publications)
Species: Hu
Applications: WB, AgAct
ICOS [Unconjugated]
169-CS
Recombinant Human ICOS Fc Chimera (Catalog # 169-CS) inhibits rhB7-H2/FcChimera (Catalog # 165-B7) induced human T cell proliferation in the presence of anti-CD3. The ED50 for this effect is 1‑4 µg/mL.
(6 Reviews)
(2 Publications)
Species: Hu
IFN-gamma
[Unconjugated]
285-IF
Recombinant Human IFN-gamma (Catalog # 285‑IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The activity is over 2-fold greater than the top competitor's IFN-gamma.
1 μg/lane of Recombinant Human IFN-gamma was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.
(19 Reviews)
(333 Publications)
Species: Hu
TNF-alpha
[Unconjugated]
210-TA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
(32 Reviews)
(645 Publications)
Species: Hu
FoxP3 Antibody
NB100-39002
Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Mouse spleen stained with FoxP3 antibody. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Tissue section of human tonsil using 1:200 dilution of rabbit anti-FoxP3 antibody. The staining was developed with HRP-labeled anti-rabbit IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This FoxP3 antibody generated an expected and a specific nuclear staining of FOXP3 in a subset of cells which are potentially the Treg cells.
Immunohistochemistry: FoxP3 Antibody [NB100-39002] - Antibody was tested in mouse spleen using DAB with hematoxylin counterstain.
(2 Reviews)
(22 Publications)
Species: Hu, Mu, Rt, Bv
Applications: WB, Flow, IB, ICC/IF, IHC, IHC-P, IP, Single-Cell Western
CD25/IL-2R alpha
[HRP]
DR2A00
(31 Publications)
Species: Hu
ISG20 Antibody
(1B2-3C9)
H00003669-M01
Western Blot: ISG20 Antibody (1B2-3C9) [H00003669-M01] - Analysis of ISG20 expression in transfected 293T cell line by ISG20 monoclonal antibody (M01), clone 1B2-3C9.Lane 1: ISG20 transfected lysate(20.4 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: ISG20 Antibody (1B2-3C9) [H00003669-M01] - Analysis of monoclonal antibody to ISG20 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: ISG20 Antibody (1B2-3C9) [H00003669-M01] - Detection limit for recombinant GST tagged ISG20 is approximately 0.1ng/ml as a capture antibody.
(2 Publications)
Species: Hu
Applications: WB, ELISA, ICC/IF, S-ELISA
TIM-3 Antibody
[Unconjugated]
AF2365
TIM‑3 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human TIM‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2365) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and extracellular space. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
TIM-3 mRNA was detected in formalin-fixed paraffin-embedded tissue sections of human tonsil probed with ACD RNAScope® Probe (Catalog # 560681) and stained using ACD RNAscope® 2.5 HD Detection Reagents-Red (top image, Catalog # 32260). Adjacent tissue section was processed for immunohistochemistry using R&D Systems Goat Anti-Human TIM-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2365) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC004) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue).
(3 Reviews)
(15 Publications)
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, Dual ISH-IHC
B7-2/CD86 Antibody
(BU63)
NBP2-25208
Knockout Validated: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Western blot shows lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2 knockout (KO) Ramos cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human B7-2 Monoclonal Antibody (Catalog # NBP2-25208) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for B7-2 at approximately 90 kDa (as indicated) in the parental Ramos cell line, but is not detectable in the knockout Ramos cell line. This experiment was conducted under reducing conditions.
Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Immunofluorescence staining of PFA-fixed Ramos cells using B7-2/CD86 Antibody (BU63) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.
Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Formalin-fixed, paraffin-embedded esophagus tumor tissue stained with CD86 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Note strong staining of differentiated squamous cells. TMA was used for this test.
(11 Publications)
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO
IL-4 [Unconjugated]
6507-IL/CF
IL-4 [Unconjugated]
(3 Publications)
Species: Hu

Publications for PD-1 Antibody (NBP1-43110H) (0)

There are no publications for PD-1 Antibody (NBP1-43110H).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PD-1 Antibody (NBP1-43110H) (0)

There are no reviews for PD-1 Antibody (NBP1-43110H). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for PD-1 Antibody (NBP1-43110H) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-43110AF350
Alexa Fluor 405 NBP1-43110AF405
Alexa Fluor 488 NBP1-43110AF488
Alexa Fluor 532 NBP1-43110AF532
Alexa Fluor 594 NBP1-43110AF594
Alexa Fluor 647 NBP1-43110AF647
Alexa Fluor 700 NBP1-43110AF700
Alexa Fluor 750 NBP1-43110AF750
Allophycocyanin NBP1-43110APC
Allophycocyanin/Cy7 NBP1-43110APCCY7
Biotin NBP1-43110B
DyLight 350 NBP1-43110UV
DyLight 405 NBP1-43110V
DyLight 488 NBP1-43110G
DyLight 550 NBP1-43110R
DyLight 594 NBP1-43110DL594
DyLight 650 NBP1-43110C
DyLight 680 NBP1-43110FR
DyLight 755 NBP1-43110IR
FITC NBP1-43110F
HRP NBP1-43110H
Janelia Fluor 549 NBP1-43110JF549
Janelia Fluor 646 NBP1-43110JF646
PE NBP1-43110PE
PE NBP1-43700
PE NBP1-43700
PE/Atto594 NBP1-43110PEATT594
PE/Cy5.5 NBP1-43110PECY55
PE/Cy7 NBP1-43110PECY7
PerCP NBP1-43110PCP
Learn about Custom Antibody Labeling

Additional PD-1 Products

Bioinformatics Tool for PD-1 Antibody (NBP1-43110H)

Discover related pathways, diseases and genes to PD-1 Antibody (NBP1-43110H). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PD-1 Antibody (NBP1-43110H)

Discover more about diseases related to PD-1 Antibody (NBP1-43110H).
 

Pathways for PD-1 Antibody (NBP1-43110H)

View related products by pathway.

PTMs for PD-1 Antibody (NBP1-43110H)

Learn more about PTMs related to PD-1 Antibody (NBP1-43110H).
 

Research Areas for PD-1 Antibody (NBP1-43110H)

Find related products by research area.

Blogs on PD-1.

Check out the latest blog posts on PD-1.
Thomson Reuters Predicts 2016 Nobel Prize Winners
Here at Bio-Techne we always look forward to the annual announcements of winners of the highly coveted Nobel Prize – the greatest award in science. How can you go about predicting which scientists might be in line for a life-changing phone-call fro...  Read full blog post.
Coronavirus Brochure

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005
When Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) is coated at 0.1 μg/mL, Recombinant Human B7-H1/PD‑L1 Fc Chimera (Catalog # 156-B7) binds with a typical ED50 of 0.15-0.75 μg/mL.
PD-1 [Unconjugated]
1086-PD-050

Contact Information

Learn the difference between western blot and simple western

Product PDFs

  PDF Datasheet
  Request CofA
  Request SDS

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PD-1 Antibody (J43) [HRP] and receive a gift card or discount.


Bioinformatics

Gene Symbol PDCD1