![ELISA: Human PCNA ELISA Kit (Colorimetric) [NBP3-18219] - Standard Curve Reference](http://images.novusbio.com/fullsize/Human-PCNA-ELISA-Kit-Colorimetric-ELISA-NBP3-18219-img0001.jpg "ELISA: Human PCNA ELISA Kit (Colorimetric) [NBP3-18219] - Standard Curve Reference")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA |
| Suitable Sample Type | Serum, plasma and cell culture supernates |
| Standard Curve Range | 0.39-25 ng/ml |
| Sensitivity | 0.055 ng/ml |
| Description | The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human PCNA in the sample, this Human PCNA ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human PCNA concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve. |
| Standard Curve Range | 0.39-25 ng/ml |
| Sensitivity | 0.055 ng/ml |
| Assay Type | Sandwich ELISA |
| Inter-Assay | %CV < 15 |
| Intra-Assay | %CV < 10 |
| Spike Recovery | 87-99% |
| Sample Volume | 100 ul |
| Kit Type | ELISA Kit (Colorimetric) |
| Gene | PCNA |
| Storage | Storage of components varies. See protocol for specific instructions. |
| Components |
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The relationship between Ki67 and HIF-1 in cancer Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life. First discovered to be located to dividing cells,... Read full blog post. |
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Caspase 3 - a Reliable Marker for Index of Apoptosis Induction Caspase-3 is one of the most important players in apoptosis signaling. It is synthesized as an inactive 32 kDa pro-enzyme and upon direct activation by Caspase-8, -9 or -10, it gets processed into its active forms, the p17-20 and p10-12 subunits. T... Read full blog post. |
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PCNA (Proliferating cell nuclear antigen, polymerase delta auxiliary protein) PCNA is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It coordinates the recruitment and association of needed components during both of these processes, both of which are essential for cell cycl... Read full blog post. |
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PCNA is a Universal Marker of Proliferating Cells Proliferating cell nuclear antigen (PCNA) is an evolutionarily well-conserved protein found in all eukaryotic species as well as in Archaea. PCNA was first shown to be involved in DNA replication. However PCNA functions are associated with other vital... Read full blog post. |
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| Gene Symbol | PCNA |
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