PCNA associated factor Antibody - BSA Free

Western Blot: PCNA associated factor Antibody [NBP2-32112] - Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-PAF antibody NBP2-32112 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry-Paraffin: PCNA associated factor Antibody [NBP2-32112] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PAF used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

• Reactivity: Hu
• Applications: WB, IHC, IP (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PCNA associated factor Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PCNA associated factor Antibody - BSA Free (NBP2-32112) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen this antibody was made to, maps to a region between residue 61 to 111 of human PCNA-Associated Factor using the numbering given in entry NP_055551.1 (GeneID 9768).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PCLAF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:2000 - 1:10000
  • Immunohistochemistry-Paraffin 1:2000 - 1:10000
  • Western Blot 1:2000 - 1:10000
• Application Notes: Immunoprecipitation Not recommended. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PCNA associated factor Antibody - BSA Free

• HCV NS5A-transactivated protein 9
• Hepatitis C virus NS5A-transactivated protein 9
• KIAA0101
• NS5ATP9p15PAF
• OEATC-1OEATC1
• Overexpressed in anaplastic thyroid carcinoma 1
• p15(PAF)
• PAF
• PCNA-associated factor

Background

KIAA0101/NS5ATP9/PAF/p15(PAF) is involved in protection of cells from UV-induced cell death and is also a Hepatitis C NS5A up-regulation gene which may play a role in the pathogenesis of HCV-associated hepatocellular carcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PCLAF
• Uniprot:
  • NP_055551.1(Human)