PCNA Antibody


Western Blot: PCNA Antibody [NB100-475] - Use at a dilution of 1:1,000.

Product Details

Reactivity MuSpecies Glossary
Applications WB
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

PCNA Antibody Summary

Recombinant fusion protein expressed in E.coli from PCNA interdomain connector loop protein. This immunogen is comprised of residues 107-196 of human PCNA. (The C-terminal portion of this antigen does not extend beyond the true IDCL of PCNA, and as such, also includes part of the PCNA D2E2 loop and the protein in between.)
Proliferation Marker
This partiular antiserum was raised against the interdomain connector loop region of PCNA, which is the region of PCNA through which many of the protein-protein interactions occur.
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Preservative: None Constituents: 10mM PBS. pH 7.4
No Preservative
This product is unpurified. The exact concentration of antibody is not quantifiable.


Application Notes
WB: Use at a dilution of 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31.3 kDa). Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. Use anti-mouse IgG secondary.

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.


This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Alternate Names for PCNA Antibody

  • cyclin
  • DNA polymerase delta auxiliary protein
  • MGC8367
  • proliferating cell nuclear antigen


PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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PCNA Antibodies: Marking Cell Proliferation & DNA Replication
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PCNA antibodies are useful biomarkers in DNA repair studies. PCNA is one of several proteins essential for the completion of nucleotide excision repair, a multi-stage process involving 20 - 30 proteins, and an important factor in repairing damage and...  Read full blog post.

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Gene Symbol PCNA

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