PCMT1 Antibody (4G9) - Azide and BSA Free

Western Blot: PCMT1 Antibody (4G9) [H00005110-M01] - PCMT1 monoclonal antibody (M01), clone 4G9 Analysis of PCMT1 expression in PC-12.

• Reactivity: Hu, Rt
• Applications: WB, ELISA
• Clone: 4G9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PCMT1 Antibody (4G9) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PCMT1 (AAH07501.1, 1 a.a. ~ 228 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAWKSGGASHSELIHNLRKNGIIKTDKVFEVMLATDRSHYAKCNPYMDSPQSIGFQATISAPHMHAYALELLFDQLHEGAKALDVGSGSGILTACFARMVGCTGKVIGIDHIKELVDDSVNNVRKDDPTLLSSGRVQLVVGDGRMGYAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLILPVGPAGGNQMLEQYDKLQDGSIKMKPLMGVIYVPLTDKEKQWSRDEL
• Specificity: PCMT1 - protein-L-isoaspartate (D-aspartate) O-methyltransferase
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PCMT1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PCMT1 Antibody (4G9) - Azide and BSA Free

• EC 2.1.1
• EC 2.1.1.77
• L-isoaspartyl protein carboxyl methyltransferase
• L-isoaspartyl/D-aspartyl methyltransferase
• PIMT
• Protein L-isoaspartyl/D-aspartyl methyltransferase
• Protein-beta-aspartate methyltransferase
• protein-L-isoaspartate (D-aspartate) O-methyltransferase
• protein-L-isoaspartate(D-aspartate) O-methyltransferase

Background

Three classes of protein carboxyl methyltransferases, distinguished by their methyl-acceptor substrate specificity, have been found in prokaryotic and eukaryotic cells. The type II enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the free carboxyl groups of D-aspartyl and L-isoaspartyl residues. These methyl-accepting residues result from the spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues and represent sites of covalent damage to aging proteins PCMT1 (EC 2.1.1.77) is a protein repair enzyme that initiates the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PCMT1
• Entrez:
  • 5110(Human)
• OMIM:
  • 176851(Human)
• Uniprot:
  • AAH07501(Human)