PCMT1 Antibody (1D6) - Azide and BSA Free

Western Blot: PCMT1 Antibody (1D6) [H00005110-M02] - PCMT1 monoclonal antibody (M02), clone 1D6. Analysis of PCMT1 expression in NIH/3T3.

Western Blot: PCMT1 Antibody (1D6) [H00005110-M02] - PCMT1 monoclonal antibody (M02), clone 1D6 Analysis of PCMT1 expression in HeLa.

Western Blot: PCMT1 Antibody (1D6) [H00005110-M02] - PCMT1 monoclonal antibody (M02), clone 1D6. Analysis of PCMT1 expression in PC-12.

Western Blot: PCMT1 Antibody (1D6) [H00005110-M02] - PCMT1 monoclonal antibody (M02), clone 1D6. Analysis of PCMT1 expression in Raw 264.7.

Sandwich ELISA: PCMT1 Antibody (1D6) [H00005110-M02] - Detection limit for recombinant GST tagged PCMT1 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clone: 1D6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PCMT1 Antibody (1D6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PCMT1 (NP_005380, 117 a.a. ~ 225 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DDSVNNVRKDDPTLLSSGRVQLVVGDGRMGYAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLILPVGPAGGNQMLEQYDKLQDGSIKMKPLMGVIYVPLTDKEKQWSR
• Specificity: PCMT1 - protein-L-isoaspartate (D-aspartate) O-methyltransferase
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PCMT1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PCMT1 Antibody (1D6) - Azide and BSA Free

• EC 2.1.1
• EC 2.1.1.77
• L-isoaspartyl protein carboxyl methyltransferase
• L-isoaspartyl/D-aspartyl methyltransferase
• PIMT
• Protein L-isoaspartyl/D-aspartyl methyltransferase
• Protein-beta-aspartate methyltransferase
• protein-L-isoaspartate (D-aspartate) O-methyltransferase
• protein-L-isoaspartate(D-aspartate) O-methyltransferase

Background

Three classes of protein carboxyl methyltransferases, distinguished by their methyl-acceptor substrate specificity, have been found in prokaryotic and eukaryotic cells. The type II enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the free carboxyl groups of D-aspartyl and L-isoaspartyl residues. These methyl-accepting residues result from the spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues and represent sites of covalent damage to aging proteins PCMT1 (EC 2.1.1.77) is a protein repair enzyme that initiates the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PCMT1
• Entrez:
  • 5110(Human)
• OMIM:
  • 176851(Human)
• Uniprot:
  • NP_005380(Human)