Pax4 Antibody

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Western Blot: PAX4 Antibody [NBP1-49863] - Analysis of PAX4 in Mouse Pancreas lysate (35ug protein in RIPA buffer) using NBP1-49863 at 0.2ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
ELISA: PAX4 Antibody [NBP1-49863] - (1.5ug/ml) as the reporter used with the capture rabbit antibody (5ug/ml)

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, EIA, ELISA, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

Pax4 Antibody Summary

Immunogen
Peptide with sequence C-GKLATATSLPEDTVR corresponding to internal region according to NP_006184.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PAX4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 - 0.6 ug/ml
  • ELISA 1:100 - 1:2000
  • Enzyme Immunoassay 1 - 2 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
EIA: Sandwich ELISA with increasing amount of recombinant PAX4 captured by a rabbit antibody. WB: Approx. 37 kDa band observed in mouse pancreas lysates and in lysates of A431, HeLa and K562 (calculated MW of 37.1 kDa band according to human NP_006184.2 and 38.0 kDa band according to mouse NP_035168.1).
Publications
Read Publication using NBP1-49863.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Pax4 Antibody

  • KPD
  • MGC129960
  • MODY9paired box protein Pax-4
  • paired box 4
  • paired box gene 4
  • paired domain gene 4
  • Pax4

Background

This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The paired box 4 gene is involved in pancreatic islet development and mouse studies have demonstrated a role for this gene in differentiation of insulin-producing beta cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Pax4 Antibody (NBP1-49863)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on Pax4.

Using EGF Protein from Novus Biologicals
EGF (epidermal growth factor) stimulates differentiation, proliferation and cell growth by binding to its receptor, EGFR. EGF was first discovered in the mouse submandibular gland in 1986 by Stanley Cohen of Vanderbilt University, leading to a Nobel P...  Read full blog post.

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Bioinformatics

Gene Symbol PAX4
Entrez
Uniprot