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Pax4 Antibody (OTI2F3) [Alexa Fluor® 488]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
OTI2F3
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 488

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-73257AF350
Alexa Fluor 405
NBP2-73257AF405
Alexa Fluor 532
NBP2-73257AF532
Alexa Fluor 594
NBP2-73257AF594
Alexa Fluor 647
NBP2-73257AF647
Alexa Fluor 700
NBP2-73257AF700
Alexa Fluor 750
NBP2-73257AF750
Biotin
NBP2-73257B
DyLight 350
NBP2-73257UV
DyLight 405
NBP2-73257V
DyLight 488
NBP2-73257G
DyLight 550
NBP2-73257R
DyLight 594
NBP2-73257DL594
DyLight 650
NBP2-73257C
DyLight 680
NBP2-73257FR
DyLight 755
NBP2-73257IR
HRP
NBP2-73257H
Unconjugated
NBP2-46334
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-46334
Azide and BSA Free
NBP2-73257
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Pax4 Antibody (OTI2F3) [Alexa Fluor® 488] Summary

Immunogen
Recombinant protein fragment corresponding to amino acids 1-228 of human Pax4 (NP_006184) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PAX4
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Pax4 Antibody (OTI2F3) [Alexa Fluor® 488]

  • KPD
  • MGC129960
  • MODY9paired box protein Pax-4
  • paired box 4
  • paired box gene 4
  • paired domain gene 4
  • Pax4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-34260
Western Blot: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Probing of Western blots of pancreatic lysates from control and iron overloaded Tg40 PrP and PrP-/- mice with antibodies specific for insulin dimer and pentamer (upper and lower panels) shows decreased expression in Tg40 PrP relative to PrP-/- samples (lanes 1 & 5 vs. 3 & 7). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 & 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 & 6). The difference is minimal in PrP-/- samples (panels 7 & 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)
(5 Reviews)
(1 Publication)
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
PDX-1/IPF1
Antibody [Unconjugate
AF2419
Western blot shows lysates of beta TC‑6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PDX‑1/IPF1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
PDX‑1/IPF1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic progenitor cells using Goat Anti-Human PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Simple Western lane view shows lysates of beta TC‑6 mouse beta cell insulinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PDX‑1/IPF1 at approximately 48 kDa (as indicated) using 10 µg/mL of Goat Anti-Human PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
(3 Reviews)
(23 Publications)
Species: Hu
Applications: WB, Simple Western, IHC, ICC
Neurogenin-3
Antibody
AF3444
Western blot shows lysates of human placenta and prostate tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Neurogenin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3444) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neurogenin‑3 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
(8 Publications)
Species: Hu
Applications: WB
Pax6 Antibody
NB300-750
Western Blot: Pax6 Antibody [NB300-750] - Analysis of sheep retinal extract
Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NB300-750] - ARPE-19 monolayer on glass substrate. Fixative Methanol Blocking Goat Serum. Dilution 1:100. Incubation 1h @ RT. This image was submitted via customer Review.
Western Blot: Pax6 Antibody [NB300-750] - Analysis of rat whole eye extract
(4 Reviews)
(4 Publications)
Species: Hu, Mu, Rt, Fi, Pm, Pm, Sh
Applications: WB, ICC/IF, IHC, IP
NeuroD1 Antibody
(3H8)
H00004760-M01
Western Blot: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of NEUROD1 expression in transfected 293T cell line by NEUROD1 monoclonal antibody (M01), clone 3H8.Lane 1: NEUROD1 transfected lysate(39.9 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of monoclonal antibody to NEUROD1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of monoclonal antibody to NEUROD1 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. Antibody concentration 3 ug/ml.
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Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
PDHX Antibody
NBP1-84079
Western Blot: PDHX Antibody [NBP1-84079] - Analysis using Anti-PDHX antibody NBP1-84079 (A) shows similar pattern to independent antibody NBP1-84080 (B).
Immunohistochemistry-Paraffin: PDHX Antibody [NBP1-84079] - Staining of human kidney.
Immunohistochemistry-Paraffin: PDHX Antibody [NBP1-84079] - Staining of human stomach shows strong cytoplasmic(granular pattern) and nuclear positivity in glandular cells.
Species: Hu
Applications: WB, IHC, IHC-P
Glucagon Antibody
(181402) [Unconjugate ...
MAB1249
Glucagon was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using 10 µg/mL Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
beta TC‑6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
(3 Reviews)
(4 Publications)
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Applications: CyTOF-ready, ICC, ICFlow
NKX6.1 Antibody
NBP1-49672
Western Blot: NKX6.1 Antibody [NBP1-49672] - Analysis of Nkx6.1 in human skeletal muscle.
Immunocytochemistry/Immunofluorescence: NKX6.1 Antibody [NBP1-49672] - Nkx6.1 antibody was tested at 1:250 in INS-1 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40X.
Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-49672] - Paraffin embeded rat pancreatic tissue at 40x resolution stained for insulin (red) and NKX6.1 (NBP1-49672, green). IHC-P image submitted by a verified customer review.
(4 Reviews)
(10 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro
NKX2.2 Antibody
NBP1-82554
Western Blot: NKX2.2 Antibody [NBP1-82554] - Analysis in human cell line U-138 MG.
Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP1-82554] - Staining of human cerebellum shows moderate postivity in cells in molecular layer.
Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP1-82554] - Staining of human pancreas shows strong nuclear positivity in islet cells.
(2 Reviews)
(4 Publications)
Species: Hu, Mu, Fe
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Islet-1 Antibody
[Unconjugated]
AF1837
Simple Western lane view shows lysates of JOY6 human induced pluripotent stem cells undifferentiated and differentiated into motor neurons, loaded at 0.2 mg/mL. A specific band was detected for Islet‑1 at approximately 53 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Islet‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western blot shows lysates of JOY6 human induced pluripotent stem cells undifferentiated and differentiatied into motor neurons. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Islet‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Islet‑1 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Islet‑1 was detected in immersion fixed iPS2 human induced pluripotent stem cells differentiated to endocrine progenitor cells using Goat Anti-Human Islet‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(1 Review)
(11 Publications)
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Applications: WB, Simple Western, ICC
Somatostatin
Antibody (7G5)
NBP2-37447
Western Blot: Somatostatin Antibody (7G5) [NBP2-37447] - Analysis using SST mAb against human SST recombinant protein. (Expected MW is 38.2 kDa)
Immunohistochemistry-Paraffin: Somatostatin Antibody (7G5) [NBP2-37447] - Analysis of lung cancer tissues using SST mouse mAb with DAB staining.
Flow Cytometry: Somatostatin Antibody (7G5) [NBP2-37447] - Analysis of HepG2 cells using SST mouse mAb (green) and negative control (red).
(1 Review)
(1 Publication)
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready, IF
Glut2 Antibody
NBP2-22218
Western Blot: Glut2 Antibody [NBP2-22218] - Analysis of Glut2 in total lysates of MIN6 cells using anti-Glut2 (NBP2-22218) antibody at 1/500 dilution. Image was submitted via customer review.
Immunocytochemistry/Immunofluorescence: Glut2 Antibody [NBP2-22218] - Glut2 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry-Paraffin: Glut2 Antibody [NBP2-22218] - Histology and immunostaining of pancreas in treated and untreated mice with STZ. (i, iv) H-E of a pancreatic islet in untreated and STZ-WT mice; (ii, v) and (iii,vi) reduction of Glut2 and insulin expression in STZ-WT mice compared to control mice. B. ( Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0105550), licensed under a CC-BY licence.
(2 Reviews)
(7 Publications)
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
SAE2 Antibody
(OTI4A3)
NBP2-03688
Western Blot: SAE2 Antibody (4A3) [NBP2-03688] Analysis of extracts (35ug) from 9 different cell lines by using anti-SAE2 monoclonal antibody.
Immunocytochemistry/Immunofluorescence: SAE2 Antibody (4A3) [NBP2-03688] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SAE2.
Immunohistochemistry-Paraffin: SAE2 Antibody (4A3) [NBP2-03688] - Staining of paraffin-embedded Human prostate tissue using anti-SAE2 mouse monoclonal antibody.
Species: Hu, Rt, Ca, Pm
Applications: WB, ICC/IF, IHC, IHC-P
ARX Antibody
AF7068
Western blot shows lysates of U‑87 MG human glioblastoma/astrocytoma cell line, U‑118‑MG human glioblastoma/astrocytoma cell line, and human brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human ARX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7068) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ARX at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ARX was detected in immersion fixed U‑87 MG human glioblastoma/astrocytoma cell line using Sheep Anti-Human ARX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7068) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
ARX was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human ARX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7068) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of pancreatic islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(1 Publication)
Species: Hu
Applications: WB, IHC, ICC
Pancreatic
Polypeptide/PP Antibo
NB100-1793
Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Staining of paraffin embedded Human Intestine with antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunohistochemistry-Frozen: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Analysis of Pancreatic Polypeptide in mouse adult pancreas tissue. IHC-Fr image submitted by a verified customer review.
(3 Reviews)
(14 Publications)
Species: Hu, Mu, Rt, Pm
Applications: Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA
HNF-4 alpha/NR2A1
Antibody
NBP1-89679
Western Blot: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Analysis in human cell lines Caco-2 and PC-3 using anti-HNF4A antibody. Corresponding HNF4A RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Immunocytochemistry/Immunofluorescence: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Staining of human cell line CACO-2 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Frozen: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Frozen 8 um rat liver tissue section fixed in formalin. HNF-4 alpha antibody at 1:100 (green). Multiplexed with a myeloperoxidase antibody (red). DAPI staining in blue. IHC-Fr image submitted by a verified customer review.
(1 Review)
(4 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Amylin Antibody
NBP1-06579
Amylin Antibody
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P
Glucokinase/GCK
Antibody
NBP1-33144
Western Blot: Glucokinase/GCK Antibody [NBP1-33144] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glucokinase antibody [N1C1-2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: Glucokinase/GCK Antibody [NBP1-33144] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glucokinase protein stained by Glucokinase antibody [N1C1-2] diluted at 1:500.Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Glucokinase/GCK Antibody [NBP1-33144] - MCF7 xenograft, using Glucokinase antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
HNF-3 beta/FoxA2
Antibody [Unconjugate ...
AF2400
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HNF‑3 beta /FoxA2 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Mouse splenocytes were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HNF‑3 beta /FoxA2/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The E-RABP promoter was detected by standard PCR.
HNF‑3 beta /FoxA2 was detected in immersion fixed endoderm differentiated BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(1 Review)
(38 Publications)
Species: Hu
Applications: WB, IHC, ChIP, ICC
Pax4 Antibody
(OTI2F3) [Alexa Fluor ...
NBP2-73257AF488
Pax4 Antibody (OTI2F3) [Alexa Fluor® 488]
Species: Hu
Applications: WB

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-73257AF350
Alexa Fluor 405 NBP2-73257AF405
Alexa Fluor 488 NBP2-73257AF488
Alexa Fluor 532 NBP2-73257AF532
Alexa Fluor 594 NBP2-73257AF594
Alexa Fluor 647 NBP2-73257AF647
Alexa Fluor 700 NBP2-73257AF700
Alexa Fluor 750 NBP2-73257AF750
Biotin NBP2-73257B
DyLight 350 NBP2-73257UV
DyLight 405 NBP2-73257V
DyLight 488 NBP2-73257G
DyLight 550 NBP2-73257R
DyLight 594 NBP2-73257DL594
DyLight 650 NBP2-73257C
DyLight 680 NBP2-73257FR
DyLight 755 NBP2-73257IR
HRP NBP2-73257H
Learn about Custom Antibody Labeling

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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HNF‑3 beta /FoxA2 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
HNF-3 beta/FoxA2 Antibody [Unconjugated]
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Bioinformatics

Gene Symbol PAX4