PARP Antibody

Product Discontinued

PARP Antibody Summary

• Immunogen: Highly purified, recombinant full-length human PARP1, expressed in a baculovirus expression system.
• Specificity: By WB, this recognizes intact PARP1 MW=113kDa, the 89kDa apoptosis-induced cleavage product, and other lower molecular weight fragments of PARP.
• Clonality: Polyclonal
• Host: Chicken
• Gene: PARP1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1 ug/ml
• Application Notes: Western Blot: 1 ug/ml using HRP/TMB colorimetric methods; <1 µg/ml for ECL<1 ug/ml for ECL.

Reactivity Notes

This antibody is cross reactive to Human, Mouse, and Rat.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera with 20mM Tris/HCl (pH 7.4) and 0.15M NaCl
• Preservative: 0.02% Sodium Azide
• Purity: Unpurified

Alternate Names for PARP Antibody

• ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)
• ADPRT 1
• ADPRT
• ADPRTADP-ribosyltransferase NAD(+)
• EC 2.4.2
• EC 2.4.2.30
• NAD(+) ADP-ribosyltransferase 1
• PARP apoptosis
• PARP
• PARP1
• PARP-1
• PARPADPRT1
• poly (ADP-ribose) polymerase 1
• poly (ADP-ribose) polymerase family, member 1
• poly [ADP-ribose] polymerase 1
• poly(ADP-ribose) polymerase
• poly(ADP-ribose) synthetase
• poly(ADP-ribosyl)transferase
• Poly[ADP-ribose] synthase 1
• PPOL
• PPOLpADPRT-1

Background

Poly-ADP-ribose metabolism plays a mayor role in a wide range of biological processes, such as maintenance of genomic stability, transcriptional regulation, energy metabolism and apoptosis. At least six poly-ADP-ribose polymerases (PARPs) enzymes have been identified in mammalians: PARP1, PARP2, PARP3, PARP4/vPARP, PARP5/Tankyrases-1 and PARP6/Tankyrases-2. Poly(ADP-ribose) polymerase-1 (PARP1) is an abundant and ubiquitous nuclear enzyme that catalyzes the NAD(+)-dependent addition of ADP-ribose polymers on a variety of nuclear proteins. During cellular apoptosis, PARP1 is cleaved into 89kDa and 24kDa fragments by caspases. During cellular necrosis, PARP1 is cleaved into several other fragments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for PARP Antibody (NB110-94449). (Showing 1 - 2 of 2 FAQ).

1. I was wondering if you would mind letting me know which product would you recommend for immunochemistry of the retina of the rat and mouse by poly (ADP-ribose) polymerase (PARP) and calpain. There are many different calpain antibodies in your website. The samples are 12-um vertical cryostst sections (fixed by PFA).
   • For PARP, I would recommend either NB120-2168 or NB100-64828. Bear in mind that they are both mouse monoclonals, and you may have to take extra steps to reduce mouse-on-mouse background.
2. Do you have any 100 coda or bigger controls?
   • I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.

Bioinformatics

• Gene Symbol: PARP1
• Entrez:
  • 142(Human)
  • 11545(Mouse)
  • 25591(Rat)