Park7/DJ-1 Antibody

Images

 
Western Blot: Park7/DJ-1 Antibody [NB100-1194] - (0.001ug/ml) staining of HeLa (A) and Jurkat (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Park7/DJ-1 Antibody [NB100-1194] - Human Cerebellum (A), Human Frontal Cortex (B), Human Hippocampus (C) Mouse Fetal Brain (D), Mouse Brain (E) and Rat Brain (F) lysates (35 ug protein in RIPA buffer). ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Park7/DJ-1 Antibody Summary

Immunogen
Peptide with sequence C-AAQVKAPLVLKD corresponding to C-Terminus according to NP_009193.2.
Localization
Cytoplasmic and Nuclear.
Specificity
Variants (NP_001116849.1; NP_009193.2) encode the same protein.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PARK7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 24 kDa band observed in HeLa and Jurkat lysates and of human brain (cerebellum, hippocampus, frontal cortex) and rodent brain lysates (calculated MW of 19.9 kDa band according to NP_001116849.1 and of NP_009193.2).
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Jurkat Lysate (NB800-PC2)
Park7/DJ-1 Lysate (NBL1-14106)
Reviewed Applications
Read 1 Review rated 3
using
NB100-1194 in the following applications:

Publications
Read Publications using
NB100-1194 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Park7/DJ-1 Antibody

  • DJ1
  • DJ-1
  • DJ1FLJ34360
  • EC 3.4
  • FLJ27376
  • FLJ92274
  • Oncogene DJ1
  • Park7
  • Parkinson disease (autosomal recessive, early onset) 7
  • Parkinson disease protein 7
  • protein DJ-1

Background

PARK7/DJ1 is a ubiquitously expressed protein involved in various cellular processes including cell proliferation, RNA-binding, and oxidative stress. The protein has been found to colocalize within a subset of pathologic tau inclusions in a diverse group of neurodegenerative disorders known as tauopathies (Rizzu et al. 2004). Defects in PARK7/DJ1 are the cause of autosomal recessive early-onset Parkinson's disease 7 (PARK7). Parkinson's disease (PD) is a complex, multifactorial disorder that typically manifests after the age of 50 years. The disease is characterized by bradykinesia, resting tremor, muscular rigidity and postural instability. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. PARK7 is characterized by onset before 40 years and slow progression. It has also been suggested that PARK7/DJ1 is a mitogen dependent oncogene product involved in Ras related signal transduction pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Park7/DJ-1 Antibody (NB100-1194)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(2)
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Human
(1)
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Showing Publications 1 - 7 of 7.
Publications using NB100-1194 Applications Species
Bitar MS, Liu C, Ziaei A et al. Decline in DJ-1 and decreased nuclear translocation of Nrf2 in Fuchs endothelial corneal dystrophy Invest Ophthalmol Vis Sci. 2012 Aug 24 [PMID: 22836768] (WB, Human) WB Human
Deeg S, Gralle M, Sroka K et al. BAG1 restores formation of functional DJ-1 L166P dimers and DJ-1 chaperone activity. J Cell Biol. 2010 Feb 22 [PMID: 20156966] (WB)

Details:
DJ-1 antibody western blot in the following figures. 1. Fig 1, transiently transfected wild type and mutant DJ-1 SH-SY5Y neuroblastoma cells, DJ-1 siRNA knockdown of endogenous DJ-1 SH-SY5Y cells, detection of DJ-1 following IP of BAG1 (results showed tha
WB
Baulac S, Lu H, Strahle J, Yang T, Goldberg MS, Shen J, Schlossmacher MG, Lemere CA, Lu Q, Xia W. Increased DJ-1 expression under oxidative stress and in Alzheimer's disease brains. Mol Neurodegener;4:12. 2009 Feb 25. [PMID: 19243613]
Saito Y, Nishio K, Ogawa Y, Kimata J, Kinumi T, Yoshida Y, Noguchi N, Niki E. Turning point in apoptosis/necrosis induced by hydrogen peroxide. Free Radic Res;40(6):619-30. 2006 Jun. [PMID: 16753840]
Ooe H, Taira T, Iguchi-Ariga SM, Ariga H. Induction of reactive oxygen species by bisphenol A and abrogation of bisphenol A-induced cell injury by DJ-1. Toxicol Sci;88(1):114-26. 2005 Nov. [PMID: 16093527]
Willis D, Li KW, Zheng JQ, Chang JH, Smit A, Kelly T, Merianda TT, Sylvester J, van Minnen J, Twiss JL. Differential transport and local translation of cytoskeletal, injury-response, and neurodegeneration protein mRNAs in axons. J Neurosci;25(4):778-91. 2005 Jan 26. [PMID: 15673657]
Nagakubo D, Taira T, Kitaura H et al. DJ-1, a novel oncogene which transforms mouse NIH3T3 cells in cooperation with ras. Biochem Biophys Res Commun 1997 Feb 13 [PMID: 9070310]

Review for Park7/DJ-1 Antibody (NB100-1194) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-1194:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 12/12/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID:22836768
SpeciesHuman

Blocking

Blocking DetailsSee PMID:22836768

Details

Detection NotesSee PMID:22836768

Comments

CommentsPublished in PMID: 22836768

Product General Protocols

View specific protocols for Park7/DJ-1 Antibody (NB100-1194): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Park7/DJ-1 Antibody (NB100-1194) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Park7/DJ-1 Antibody (NB100-1194)

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Blogs on Park7/DJ-1.

Park7/DJ-1: A Reliable Biomarker for Parkinson's Disease?
The product of the Parkinson's disease 7 (Park7/DJ-1) gene belongs to the peptidase C56 family of proteins and appears to have two transcriptional variants. It is a positive regulator of androgen receptor-dependent transcription, and some evidence sug...  Read full blog post.

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Recent Reviews

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1
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Anonymous
12/12/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol PARK7
Entrez
Uniprot