Park7/DJ-1 Antibody

Images

 
Western Blot: Park7/DJ-1 Antibody [NB100-1194] - (0.001ug/ml) staining of HeLa (A) and Jurkat (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Park7/DJ-1 Antibody [NB100-1194] - (0.001ug/ml) staining of Human Cerebellum (A), Human Frontal Cortex (B), Human Hippocampus (C) Mouse Fetal Brain (D), Mouse Brain (E) and Rat Brain (F) lysates (35ug ...read more
Immunocytochemistry/ Immunofluorescence: Park7/DJ-1 Antibody [NB100-1194] - Immunofluorescence staining of MCF7 (A) and Hela (B) cells with 5ug/ml NB100-1194 antibody. Detected with Rabbit anti-goat IgG-Alexafluor488 ...read more
Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NB100-1194] - (7ug/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NB100-1194] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Park7/DJ-1 Antibody Summary

Immunogen
Peptide with sequence C-AAQVKAPLVLKD corresponding to C-Terminus according to NP_009193.2.
Localization
Cytoplasmic and Nuclear.
Specificity
Variants (NP_001116849.1; NP_009193.2) encode the same protein.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PARK7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 5 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 7 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.5-1 ug/ml
Application Notes
WB: Approx. 24 kDa band observed in HeLa and Jurkat lysates and of human brain (cerebellum, hippocampus, frontal cortex) and rodent brain lysates (calculated MW of 19.9 kDa band according to NP_001116849.1 and of NP_009193.2).
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Jurkat Whole Cell Lysate
Park7/DJ-1 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 3
using
NB100-1194 in the following applications:

Publications
Read Publications using
NB100-1194 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Park7/DJ-1 Antibody

  • DJ1
  • DJ-1
  • DJ1FLJ34360
  • EC 3.4
  • FLJ27376
  • FLJ92274
  • Oncogene DJ1
  • Park7
  • Parkinson disease (autosomal recessive, early onset) 7
  • Parkinson disease protein 7
  • protein DJ-1

Background

PARK7/DJ1 is a ubiquitously expressed protein involved in various cellular processes including cell proliferation, RNA-binding, and oxidative stress. The protein has been found to colocalize within a subset of pathologic tau inclusions in a diverse group of neurodegenerative disorders known as tauopathies (Rizzu et al. 2004). Defects in PARK7/DJ1 are the cause of autosomal recessive early-onset Parkinson's disease 7 (PARK7). Parkinson's disease (PD) is a complex, multifactorial disorder that typically manifests after the age of 50 years. The disease is characterized by bradykinesia, resting tremor, muscular rigidity and postural instability. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. PARK7 is characterized by onset before 40 years and slow progression. It has also been suggested that PARK7/DJ1 is a mitogen dependent oncogene product involved in Ras related signal transduction pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-1194
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC

Publications for Park7/DJ-1 Antibody (NB100-1194)(8)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 1 application: WB.


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WB
(2)
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(1)
Rat
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Showing Publications 1 - 8 of 8.
Publications using NB100-1194 Applications Species
Sriram K, Lin GX, Jefferson AM et al. Biological effects of inhaled crude oil vapor V. Altered biogenic amine neurotransmitters and neural protein expression Toxicology and applied pharmacology 2022-06-21 [PMID: 35750205] (Rat) Rat
Bitar MS, Liu C, Ziaei A et al. Decline in DJ-1 and decreased nuclear translocation of Nrf2 in Fuchs endothelial corneal dystrophy Invest Ophthalmol Vis Sci. 2012-08-24 [PMID: 22836768] (WB, Human) WB Human
Deeg S, Gralle M, Sroka K et al. BAG1 restores formation of functional DJ-1 L166P dimers and DJ-1 chaperone activity. J Cell Biol. 2010-02-22 [PMID: 20156966] (WB)

Details:
DJ-1 antibody western blot in the following figures. 1. Fig 1, transiently transfected wild type and mutant DJ-1 SH-SY5Y neuroblastoma cells, DJ-1 siRNA knockdown of endogenous DJ-1 SH-SY5Y cells, detection of DJ-1 following IP of BAG1 (results showed tha
WB
Baulac S, Lu H, Strahle J, Yang T, Goldberg MS, Shen J, Schlossmacher MG, Lemere CA, Lu Q, Xia W. Increased DJ-1 expression under oxidative stress and in Alzheimer's disease brains. Mol Neurodegener;4:12. 2009-02-25 [PMID: 19243613]
Saito Y, Nishio K, Ogawa Y, Kimata J, Kinumi T, Yoshida Y, Noguchi N, Niki E. Turning point in apoptosis/necrosis induced by hydrogen peroxide. Free Radic Res;40(6):619-30. 2006-06-01 [PMID: 16753840]
Ooe H, Taira T, Iguchi-Ariga SM, Ariga H. Induction of reactive oxygen species by bisphenol A and abrogation of bisphenol A-induced cell injury by DJ-1. Toxicol Sci;88(1):114-26. 2005-11-01 [PMID: 16093527]
Willis D, Li KW, Zheng JQ, Chang JH, Smit A, Kelly T, Merianda TT, Sylvester J, van Minnen J, Twiss JL. Differential transport and local translation of cytoskeletal, injury-response, and neurodegeneration protein mRNAs in axons. J Neurosci;25(4):778-91. 2005-01-26 [PMID: 15673657]
Nagakubo D, Taira T, Kitaura H et al. DJ-1, a novel oncogene which transforms mouse NIH3T3 cells in cooperation with ras. Biochem Biophys Res Commun 1997-02-13 [PMID: 9070310]

Review for Park7/DJ-1 Antibody (NB100-1194) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-1194:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
  3
reviewed by:
Verified Customer
WB Human 12/12/2014
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Summary

ApplicationWestern Blot
Sample TestedSee PMID:22836768
SpeciesHuman

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Park7/DJ-1 Antibody (NB100-1194) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Park7/DJ-1.

Park7/DJ-1: A Reliable Biomarker for Parkinson's Disease?
The product of the Parkinson's disease 7 (Park7/DJ-1) gene belongs to the peptidase C56 family of proteins and appears to have two transcriptional variants. It is a positive regulator of androgen receptor-dependent transcription, and some evidence sug...  Read full blog post.

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Verified Customer
12/12/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol PARK7
Entrez
Uniprot