Pappalysin-1/PAPP-A Antibody (4G11)

Western Blot: PAPP A Antibody (4G11) [NB200-401] - Detection of htPAPP-A in Native human PAPP-A (PAPP-A/proMBP complex).

Sandwich ELISA: PAPP A Antibody (4G11) [NB200-401] - Calibration curves for two PAPP- A sandwich immunoassays. (A) 4G11 3C8 and (B) 4G11 10H9. Capture MAb: 4G11 (biotinylated) Detection MAbs: 3C8 or 10H9 (labeled with stable Eu3+ -chelate) Antigen: htPAPP-A

Product Discontinued

Pappalysin-1/PAPP-A Antibody (4G11) Summary

• Immunogen: Human PAPP-A antigen purified from placental blood (heterotetrameric complex, consisting of PAPP-A subunits and pro-MBP subunits).
• Localization: Secreted
• Specificity: PAPPA (4G11)
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PAPPA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoassay
  • Immunoprecipitation 1:10-1:500
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: Recommended pairs for the detection of dPAPP-A antigen in blood of AMI patients and patients with unstable angina (capture - detection): NB200-401 - NB200-403, NB200-401 - NB200-404

Reactivity Notes

Cross-reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Pappalysin-1/PAPP-A Antibody (4G11)

• ASBABP2
• DIPLA1
• EC 3.4.24.79
• IGFBP-4ase
• IGFBP-4aseaspecific BCL2 ARE-binding protein 2
• IGF-dependent IGFBP-4 protease
• insulin-like growth factor-dependent IGF binding protein-4 protease
• Insulin-like growth factor-dependent IGF-binding protein 4 protease
• PAPA
• PAPPA
• PAPPA1
• PAPP-A1
• PAPP-Adifferentially placenta 1 expressed protein
• Pappalysin1
• Pappalysin-1
• pregnacy-associated plasma protein A
• Pregnancy-associated plasma protein A
• pregnancy-associated plasma protein A, pappalysin 1

Background

PAPP A was originally isolated from pregnancy serum as heterotetrameric protein complex with molecular weight approximately 500 kDa, consisting of two PAPP A subunits disulfide-linked with two subunits of the proform of eosinophil major basic protein (proMBP). In such a complex proMBP functions as an inhibitor of PAPP A proteinase activity. PAPP A is widely used as a marker of some pathologies during pregnancy. Reduced level of PAPP A in pregnancy blood in the first trimester is associated with fetal Down syndrome. PAPP A is considered as one of the best biochemical markers in early pregnancy used to screen for Down syndrome during the first trimester (Wald, N. J. et al).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pappalysin-1/PAPP-A Antibody (NB200-401)(1)

Publication using NB200-401

• Pan H, Hanada S, Zhao J et al. Protein secretion is required for pregnancy-associated plasma protein-a to promote lung cancer growth in vivo PLoS One 2012-01-01 [PMID: 23152806] (WB, Human)

FAQs for Pappalysin-1/PAPP-A Antibody (NB200-401). (Showing 1 - 1 of 1 FAQ).

1. I am highly interested to use couple of Anti-PAPP-A antibodies from your reputed companies for my research work. I have got the information regarding the immunogen used to generate these antibodies, however could not get any information regarding the specific amino acids/epitope to which your Anti-PAPP-A Antibodies binds with the antigen (PAPP-A).
   • Unfortunately, none of our PAPP A antibodies have been epitope mapped. Many of them have also been generated against full-length PAPP A, so there are many potential binding sites.

Bioinformatics

• Gene Symbol: PAPPA
• Entrez:
  • 5069(Human)