pan RhO/RAC/CDC42 Antibody (3L74) - Azide and BSA Free

Western Blot: pan RhO/RAC/CDC42 Antibody (3L74) [NBP2-29740] - HL-60 cell lysate was probed with anti-Rho (-A, -B, -C), clone 3L74 (1:2000 dilution). Arrow indicates Rho (-A, -B, -C) (~22kDa).

• Reactivity: Hu, Rt
• Applications: WB
• Clone: 3L74
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

pan RhO/RAC/CDC42 Antibody (3L74) - Azide and BSA Free Summary

• Description: Relevant Entrez Gene Numbers: NM_001664
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: KLH-conjugated, synthetic peptide corresponding to amino acids 73-104 of human Rho (-A, -B, -C), with two terminal lysines added to increase peptide solubility.
• Specificity: Recognizes Rho (-A, -B, -C).
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Rat

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.02M phosphate buffer, pH 7.6, 0.25M NaCl, and 30% glycerol.
• Preservative: No Preservative
• Purity: Protein A or G purified

Notes

Relevant Entrez Gene Numbers: NM_001664

Alternate Names for pan RhO/RAC/CDC42 Antibody (3L74) - Azide and BSA Free

• h12
• oncogene RHO H12
• ras homolog gene family, member A
• Rho cDNA clone 12
• RHO12
• RhoA
• RHOH12ARH12ARHAAplysia ras-related homolog 12
• small GTP binding protein RhoA
• transforming protein RhoA

Background

pan RhO/RAC/CDC42 is encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P61586(Human)