pan Actin Antibody (5J11)

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Western Blot: Actin Antibody (5J11) [NBP2-25142] - NBP2-25142 was used to detect GST fusion proteins expressing each of the six human actin proteins. GST-fusion proteins encoding all six full length human actin gene ...read more
Immunocytochemistry/ Immunofluorescence: Actin Antibody (5J11) [NBP2-25142] - Beta actin was detected in NIH-3T3 cells fixed with methanol using mouse anti-mouse beta-Actin monoclonal antibody (NBP2-25142) at 1:2700 ...read more
Flow Cytometry: Actin Antibody (5J11) [NBP2-25142] - analysis of HeLa cells using mouse Monoclonal Actin antibody (Orange) and Isotype control Antibody (Blue).
Western Blot: Actin Antibody (5J11) [NBP2-25142] - Analysis of HeLa, A431, K562, MCF-7, NIH-3T3, C6, and Rat-2 cell lines.
Western Blot: Actin Antibody (5J11) [NBP2-25142] - lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF ...read more
Western Blot: Actin Antibody (5J11) [NBP2-25142] - Analysis of tissue and cell lysates probed with mouse mAb against actin, NBP2-25142, in red. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] ...read more
Immunocytochemistry/ Immunofluorescence: Actin Antibody (5J11) [NBP2-25142] - HeLa cells stained with NBP2-25142 (green) and also with the chicken polyclonal antibody to Vimentin (NB300-223, red). The Actin antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, EqSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
5J11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

pan Actin Antibody (5J11) Summary

Immunogen
An Actin preparation derived from bovine brain.
Localization
Cytoplasm > cytoskeleton.
Specificity
This antibody recognizes all six isotypes of Actin.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ACTA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:500-1:1000
  • Immunohistochemistry 1:500-1:1000
  • Western Blot 1:1000
Application Notes
This Actin (5J11) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry, and Western Blot, where a band can be seen at ~42 kDa.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 5
using
NBP2-25142 in the following applications:

Publications
Read Publications using
NBP2-25142 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50% PBS, 50% glycerol
Preservative
5mM Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for pan Actin Antibody (5J11)

  • ACTA
  • actin, alpha 1, skeletal muscle
  • alpha skeletal muscle actin
  • alpha skeletal muscle
  • alpha-actin-1
  • ASMA
  • CFTD
  • CFTDM
  • MPFD
  • NEM1
  • NEM2
  • NEM3

Background

Actin is one of the most abundant and highly conserved proteins of eukaryotes. Mammalian Actins are the product of six different genes with differing distribution patterns in cell types and in tissues. The molecular weight of all six proteins is 42 kDa, and one or more actins are found in essentially every type of crude cellular and tissue extract. As a result, antibodies to Actin are widely used. As western blotting standards, they can be used to verify that the various steps of the western blotting procedure have been performed correctly. In addition, Actin is regarded as a "housekeeping" protein which is generally not altered much in expression as a result of experimental manipulations. Thus, quantitation of the Actin band on the western is used as a standard against the band density of other proteins. The Actin isotypes were originally classified as alpha, beta and gamma forms based on their charges which resulted in different mobilities in the isoelectric focusing dimension as seen on 2-dimensional SDS-PAGE. Subsequently, the alpha spot was found to potentially contain three Actin gene products, alpha-skeletal Actin, alpha-vascular smooth muscle Actin, and alpha-cardiac muscle. The beta spot contained a single protein called simply beta Actin, while the gamma spot may contain both gamma-1 Actin and gamma-2, which are enteric and smooth muscle Actins. The six mammalian gene products are very similar as shown by a sequence alignment of the human proteins. The most closely related actin isotypes are 97% identical, while the most distantly related are 94% identical, with most of the variability at the N-terminus. With this level of identity, antibodies to any one protein may recognize all six isotypes, as is the case with clone 5J11. This antibody binds all six isotypes very strongly on western blots, and it is a very effective blotting standard which can work on any cell type or tissue extract. It also works in immunocytochemical experiments, binding strongly and cleanly to filopodia, membrane ruffles and stress fibers, all known to be rich in Actin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for pan Actin Antibody (NBP2-25142)(2)

We have publications tested in 1 confirmed species: Bovine.

We have publications tested in 3 applications: B/N, In Vivo, WB.


Filter By Application
B/N
(1)
In Vivo
(1)
WB
(1)
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Bovine
(1)
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Reviews for pan Actin Antibody (NBP2-25142) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP2-25142:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Alissa Taylor
WB Human 09/24/2014
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman
Western Blot pan Actin NBP2-25142
Enlarge
5
reviewed by:
Verified Customer
WB Human 06/17/2014
View

Summary

ApplicationWestern Blot
Sample Tested293T and HepG2 lysates
SpeciesHuman

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for pan Actin Antibody (NBP2-25142) (0)

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Secondary Antibodies

 

Isotype Controls

Additional pan Actin Products

Array NBP2-25142

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Research Areas for pan Actin Antibody (NBP2-25142)

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Blogs on pan Actin.

"Actin the Fool" about Cytoskeleton Structure
Actins are highly conserved, commonly found and abundant proteins involved in several types of cell motility as well as cytoskeleton maintenance. In vertebrate species, three main groups of actin isoforms, the alpha, beta and gamma, have been identifi...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

 
Alissa Taylor
09/24/2014
Application: WB
Species: Human

Verified Customer
06/17/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol ACTA1