pan Actin Antibodies
We offer pan Actin Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot. Each pan Actin Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our pan Actin Antibodies can be used in a variety of model species: Amphibian, Bovine, Canine, Chicken, Chinese Hamster, Feline, Goat, Guinea Pig, Human, Mouse, Plant, Porcine, Primate, Protozoa, Rabbit, Rat, Reptile, Sheep. Use the list below to choose the pan Actin Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our pan Actin polyclonal antibodies and browse our pan Actin monoclonal antibody catalog.
Alternate Names for pan Actin Antibodies
anti-pan Actin antibody, anti-ACTA1 antibody, anti-ACTA antibody, anti-actin, alpha 1, skeletal muscle antibody, anti-alpha skeletal muscle actin antibody, anti-alpha skeletal muscle antibody, anti-alpha-actin-1 antibody, anti-ASMA antibody, anti-CFTD antibody, anti-CFTDM antibody, anti-MPFD antibody, anti-NEM1 antibody, anti-NEM2 antibody, anti-NEM3 antibody
3 Results for "pan-actin" in Primary Antibodies
![Western Blot: Actin Antibody (5J11) [NBP2-25142] - NBP2-25142 was used to detect GST fusion proteins expressing each of the six human actin proteins. GST-fusion proteins encoding all six full length human actin gene products were run out on SDS-PAGE, transferred to nitrocellulose and reacted with NBP2-25142. The antibody stained all six proteins indicating that it is a panspecific actin antibody.](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Western-Blot-NBP2-25142-img0012.jpg "Western Blot: Actin Antibody (5J11) [NBP2-25142] - NBP2-25142 was used to detect GST fusion proteins expressing each of the six human actin proteins. GST-fusion proteins encoding all six full length human actin gene products were run out on SDS-PAGE, transferred to nitrocellulose and reacted with NBP2-25142. The antibody stained all six proteins indicating that it is a panspecific actin antibody.")
![Immunocytochemistry/Immunofluorescence: Actin Antibody (5J11) [NBP2-25142] - Beta actin was detected in NIH-3T3 cells fixed with methanol using mouse anti-mouse beta-Actin monoclonal antibody (NBP2-25142) at 1:2700 dilution. Cells were stained using Northern Lights 557 conjugated anti-mouse secondary antibody (NL007) and counterstained with DAPI.](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Immunocytochemistry-Immunofluorescence-NBP2-25142-img0010.jpg)
![Flow Cytometry: Actin Antibody (5J11) [NBP2-25142] - analysis of HeLa cells using mouse Monoclonal Actin antibody (Orange) and Isotype control Antibody (Blue).](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Flow-Cytometry-NBP2-25142-img0008.jpg)
![Western Blot: Actin Antibody (5J11) [NBP2-25142] - Analysis of HeLa, A431, K562, MCF-7, NIH-3T3, C6, and Rat-2 cell lines.](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Western-Blot-NBP2-25142-img0007.jpg)
![Western Blot: Actin Antibody (5J11) [NBP2-25142] - lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.2 ug/mL mouse anti-Actin monoclonal (NBP2-25142, Novus Biologicals), followed by 1:2000 dilution of HRP- conjugated secondary antibody, donkey anti-mouse IgG (HAF018, R&D Systems).](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Western-Blot-NBP2-25142-img0009.jpg)
![Western Blot: Actin Antibody (5J11) [NBP2-25142] - Analysis of tissue and cell lysates probed with mouse mAb against actin, NBP2-25142, in red. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The same blot was simultaneously probed with chicken pAb to UCHL-1, a marker of neuronal lineage cells, in green.](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Western-Blot-NBP2-25142-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Antibody (5J11) [NBP2-25142] - HeLa cells stained with NBP2-25142 (green) and also with the chicken polyclonal antibody to Vimentin (NB300-223, red). The Actin antibody stains the submembranous Actin rich cytoskeleton and also stress fibers, bundles of Actin associated with adhesion sites. The Vimentin antibody stains a quite different cytoskeletal network, the intermediate filaments. The blue stain reveals DNA in the nuclei of these cells.](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Immunocytochemistry-Immunofluorescence-NBP2-25142-img0003.jpg)
![Western Blot: pan Actin Antibody (AC-40) [NB120-11003] - Cell line lysates lanes: 1. HeLa, 2. JURKAT, 3. COS7, 4. NIH-3T3, 5. PC-12, 6. RAT2, 7. CHO, 8. MDBK, 9. MDCK.](http://images.novusbio.com/fullsize/pan-Actin-Antibody-AC-40-Western-Blot-NB120-11003-img0004.jpg "Western Blot: pan Actin Antibody (AC-40) [NB120-11003] - Cell line lysates lanes: 1. HeLa, 2. JURKAT, 3. COS7, 4. NIH-3T3, 5. PC-12, 6. RAT2, 7. CHO, 8. MDBK, 9. MDCK.")
![Immunocytochemistry/Immunofluorescence: pan Actin Antibody (AC-40) [NB120-11003] - CFB cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with Monoclonal Anti-Actin produced in mouse clone No. AC-40. The antibody was developed using Anti-Mouse IgG (Fab specific)-FITC antibody produced in goat. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/pan-Actin-Antibody-AC-40-Immunocytochemistry-Immunofluorescence-NB120-11003-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: pan Actin Antibody (AC-40) [NB120-11003] - conserved C-terminus specific Monoclonal staining of human tongue frozen section with Monoclonal Anti-Actin](http://images.novusbio.com/fullsize/pan-Actin-Antibody-AC-40-Immunocytochemistry-Immunofluorescence-NB120-11003-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: pan Actin Antibody (AC-40) [NB120-11003] - Rat2 cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:250 Monoclonal Anti-Actin Clone: AC-40. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/pan-Actin-Antibody-AC-40-Immunocytochemistry-Immunofluorescence-NB120-11003-img0005.jpg)
![Western Blot: pan Actin Antibody (A2E1-R) [NBP3-32712] - Western blot analysis of pan Actin on different lysates with Mouse anti-pan Actin antibody (NBP3-32712) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: MCF7 cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: NIH/3T3 cell lysate (20 ug/Lane) Lane 5: PC-12 cell lysate (20 ug/Lane) Lane 6: Mouse smooth muscle tissue lysate (40 ug/Lane) Lane 7: Rat heart tissue lysate (40 ug/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32712) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32712_mouse-pan-actin-mab-a2e1-r-2782024163166.jpg "Western Blot: pan Actin Antibody (A2E1-R) [NBP3-32712] - Western blot analysis of pan Actin on different lysates with Mouse anti-pan Actin antibody (NBP3-32712) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: MCF7 cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: NIH/3T3 cell lysate (20 ug/Lane) Lane 5: PC-12 cell lysate (20 ug/Lane) Lane 6: Mouse smooth muscle tissue lysate (40 ug/Lane) Lane 7: Rat heart tissue lysate (40 ug/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32712) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: pan Actin Antibody (A2E1-R) [NBP3-32712] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Mouse anti-pan Actin antibody (NBP3-32712) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32712) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32712_mouse-pan-actin-mab-a2e1-r-29820241492695.jpg)
![Immunocytochemistry/ Immunofluorescence: pan Actin Antibody (A2E1-R) [NBP3-32712] - Immunocytochemistry analysis of HeLa cells labeling pan Actin with Mouse anti-pan Actin antibody (NBP3-32712) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-pan Actin antibody (NBP3-32712) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32712_mouse-pan-actin-mab-a2e1-r-29202410334034.jpg)