pan Actin Antibody (5J11) [DyLight 594]

pan Actin Antibody (5J11) [DyLight 594] [NBP2-25142DL594] - Vial of DyLight 594 conjugated antibody. DyLight 594 is optimally excited at 593 nm by the Yellow-Green laser (561 nm) and has an emission maximum of 618 nm.

• Reactivity: Hu, Mu, Rt, Po, Bv, Eq
• Applications: WB, Flow, ICC/IF, IHC
• Clone: 5J11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 594

pan Actin Antibody (5J11) [DyLight 594] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: An Actin preparation derived from bovine brain.
• Localization: Cytoplasm > cytoskeleton.
• Specificity: This antibody recognizes all six isotypes of Actin.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP2-25142. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for pan Actin Antibody (5J11) [DyLight 594]

• ACTA
• actin, alpha 1, skeletal muscle
• alpha skeletal muscle actin
• alpha skeletal muscle
• alpha-actin-1
• ASMA
• CFTD
• CFTDM
• MPFD
• NEM1
• NEM2
• NEM3

Background

Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility (1). Polymerization of globular actin (G-actin) leads to a structural filament (F-actin), whose formation requires the Arp2/3 complex (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). It has also been shown that actin dysfunction leads to certain types of heart failure (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACTA1