PAK4 Antibody [Unconjugated]

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF-7 human breast cancer cell line, PT18 mouse mast/basophil cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse/Rat PAK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4178) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PAK4 at approximately 72 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Simple Western shows lysates of MCF‑7 human breast cancer cell line, loaded at 0.5 mg/ml. A specific band was detected for PAK4 at approximately 66 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat PAK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4178). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

PAK4 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human PAK4
  Met1-Glu120
  Accession # O96013
• Specificity: Detects endogenous human, mouse and rat PAK4 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PAK4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 5 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PAK4 Antibody [Unconjugated]

• EC 2.7.11
• EC 2.7.11.1
• KIAA1142
• p21 protein (Cdc42/Rac)-activated kinase 4
• p21(CDKN1A)-activated kinase 4
• p21-activated kinase 4
• PAK4
• PAK-4
• protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs
• serine/threonine-protein kinase PAK 4

Background

As downstream targets of the Rho GTPases, the p21-activated kinase (PAK) family of serine/threonine kinases regulates the organization of the actin cytoskeleton in mammalian cells. The PAK family is structurally categorized in two groups, each with three members: group I PAK1-3; and group II PAK4-6. Targeted disruption of PAK4 in mice leads to severe defects in the development and migration of neurons, suggesting a key role for this kinase in neuronal development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAK4
• Uniprot:
  • O96013()