PAK4+5+6 [p Ser474, p Ser560, p Ser602] Antibody (2D1) - BSA Free

Western Blot: PAK4+5+6 [p Ser474, p Ser560, p Ser602] Antibody (2D1) [NBP3-15234] - Positive WB detected in 293 whole cell lysate (treated with Calyculin A or not). All lanes PAK4+5+6 antibody at 2.15ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 64,75,80 KDa. Observed band size: 75,80 Kda.

Immunocytochemistry/Immunofluorescence: PAK4+5+6 [p Ser474, p Ser560, p Ser602] Antibody (2D1) [NBP3-15234] - Immunofluorescence staining of HepG2 cells (treated with 50mM Calyculin A for 30 minutes) with at PAK4+5+6 antibody 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 2D1
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PAK4+5+6 [p Ser474, p Ser560, p Ser602] Antibody (2D1) - BSA Free Summary

• Description: Novus Biologicals Rabbit PAK4+5+6 [p Ser474, p Ser560, p Ser602] Antibody (2D1) - BSA Free (NBP3-15234) is a recombinant monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from human Phospho-PAK4+5+6 (S474/S560/S602). UniProt (O96013/Q9P286/Q9NQU5).
• Modification: p Ser474, p Ser560, p Ser602
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PAK4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:20-1:200
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for PAK4+5+6 [p Ser474, p Ser560, p Ser602] Antibody (2D1) - BSA Free

• EC 2.7.11
• EC 2.7.11.1
• KIAA1142
• p21 protein (Cdc42/Rac)-activated kinase 4
• p21(CDKN1A)-activated kinase 4
• p21-activated kinase 4
• PAK-4
• protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs
• serine/threonine-protein kinase PAK 4

Background

Three recently identified isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiaekinase STE20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is MEK kinase, an upstream effector of MEK4 which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP-dependent manner with Rac1 and Cdc42, they do not interact with Rho.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAK4