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PAK1 Antibody

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PAK1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human PAK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7495) at 15 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

PAK1 Antibody Summary

Immunogen
E. coli-derived recombinant human PAK1
Leu128-Gln242
Accession # Q13153
Specificity
Detects human PAK1 in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) PAK1B, rhPAK2, rhPAK4, rhPAK6, and rhPAK7 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PAK1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PAK1 Antibody

  • alpha-PAK
  • EC 2.7.11
  • EC 2.7.11.1
  • MGC130000
  • MGC130001
  • p21 protein (Cdc42/Rac)-activated kinase 1
  • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)
  • p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
  • p21-activated kinase 1
  • p65-PAK
  • PAK1
  • PAK-1
  • PAKalpha
  • serine/threonine-protein kinase PAK 1
  • STE20 homolog, yeast

Background

PAK1 (p21-activaed kinase 1; also alpha -PAK and p65-PAK) is both a cytoplasmic and nuclear 58-60 kDa member of PAK group I, STE20 subfamily, STE Ser/Thr protein kinase family of molecules. It is widely expressed, and is upregulated in numerous cancers. PAK1 has multiple activities. It is responsible for regulating focal adhesions during motility. At the front edge of cells, PAK1 promotes focal adhesion formation by phosphorylating PIX, while at the back edge, it directs focal adhesion disassembly. PAK1 also impacts microtubule formation. Microtubules are composed of heterodimeric subunits containing an alpha - and beta -tubulin polypeptide. The availability of alpha -tubulin for microtubule formation is based on the ability of PAK1 to phosphorylate a alpha -tubulin chaperone, tubulin cofactor B. PAK1 is activated following binding to active GTPases which induce PAK autophosphorylation. When active, PAK1 is a monomer; when inactive, it reportedly homodimerizes. Human PAK1 is 545 amino acids (aa) in length. It contains one GTPase binding domain (aa 75-105) plus a protein kinase catalytic region (aa 250-521). There are five SH3-binding motifs. Acetylation occurs on Lys256, and there are at least 14 potential phosphorylation sites, seven of which are known to be utilized. There is one splice variant that shows a 35 aa substitution for aa 518-545. Over aa 128-242, human PAK1 shares 96% aa sequence identity with mouse PAK1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PAK1
Uniprot