PAK1/2/3 [p Ser141, p Ser139, p Ser144] Antibody (1T7A5)

Western Blot: PAK1/2/3 [p Ser141, p Ser139, p Ser144] Antibody (1T7A5) [NBP3-16930] - Western blot analysis of extracts of various cell lines, using PAK1/2/3 Rabbit mAb (NBP3-16930) at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (30 uM) at 37C for 30 minutes after serum-starvation overnight Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 1T7A5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

PAK1/2/3 [p Ser141, p Ser139, p Ser144] Antibody (1T7A5) Summary

• Description: Novus Biologicals Rabbit PAK1/2/3 [p Ser141, p Ser139, p Ser144] Antibody (1T7A5) (NBP3-16930) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic phosphorylated peptide around S144 of human PAK1/2/3 (Q13153). YMSFT
• Modification: p Ser144, p Ser141, p Ser139
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PAK1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000
• Application Notes: Modifications: PAK1 - p Ser 144, PAK2 - p Ser 141, PAK3 - p Ser 139

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for PAK1/2/3 [p Ser141, p Ser139, p Ser144] Antibody (1T7A5)

• alpha-PAK
• EC 2.7.11
• EC 2.7.11.1
• MGC130000
• MGC130001
• p21 protein (Cdc42/Rac)-activated kinase 1
• p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)
• p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
• p21-activated kinase 1
• p65-PAK
• PAK-1
• PAKalpha
• serine/threonine-protein kinase PAK 1
• STE20 homolog, yeast

Background

In mammals, there are several identified isoforms of p21-activated Protein Kinases or PAKs: alpha-PAK (also known as PAK-1) and beta-PAK (also known as PAK-3) are mostly brain-specific, while gamma-PAK (also known as PAK-2) is expressed ubiquitously. Mutations of the gene coding for PAK-3 are associated with X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death. Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK. (1-4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAK1