PADI4 Antibody [FITC]

• Reactivity: Mu
• Applications: IHC, ICC/IF (-), WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: FITC

PADI4 Antibody [FITC] Summary

• Immunogen: A synthetic peptide made to an internal portion of the human PAD4 protein (between residues 200-250) [UniProt Q9UM07]
• Localization: Cytoplasm. Nucleus. Cytoplasmic granule. Note: Cytoplasmic granules of eosinophils and neutrophils.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PADI4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:400
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for PADI4 Antibody [FITC]

• HL-60 PAD
• PAD
• PAD4
• PADI4
• PADI5PADI-H protein
• PADPAD4
• PDI4
• PDI5EC 3.5.3.15
• peptidyl arginine deiminase, type IV
• peptidyl arginine deiminase, type V
• Peptidylarginine deiminase IV
• Protein-arginine deiminase type IV
• protein-arginine deiminase type-4

Background

Protein-arginine deiminase type-4 (PAD4) is a member of the protein arginine deiminase family (also known as peptidylarginine deiminase IV, PAD, PDI4, PDI5, and PADI5). Enzymes in the protein arginine deiminase family catalyze the conversion of arginine residues into citrulline residues. More specifically, PAD4 converts arginine residues to citrulline at Arg-8 and Arg-17 on histone H3 and Arg-3 on histone H4, thus preventing methylation at these sites by CARM1 and HRMT1L2/PRMT1. PAD4 also induces a favorable interaction between EP300/P300 and NCOA2/GRIP1 by citrullinating EP300/P300 at Arg-2142. In addition, the PAD4 enzyme may also be involved in the development of granulocytes and macrophages. PAD4 is expressed in the cytoplasmic granules of eosinophils and neutrophils, and is not expressed in peripheral monocytes or lymphocytes. Areas of interest involving PAD4 research include inflammation, immune response, and rheumatoid arthritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PADI4