PADI4 Antibody [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Mu
• Applications: IHC, ICC/IF (-), WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: CoraFluor 1

PADI4 Antibody [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: A synthetic peptide made to an internal portion of the human PAD4 protein (between residues 200-250) [UniProt Q9UM07]
• Localization: Cytoplasm. Nucleus. Cytoplasmic granule. Note: Cytoplasmic granules of eosinophils and neutrophils.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PADI4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for PADI4 Antibody [CoraFluor™ 1]

• HL-60 PAD
• PAD
• PAD4
• PADI4
• PADI5PADI-H protein
• PADPAD4
• PDI4
• PDI5EC 3.5.3.15
• peptidyl arginine deiminase, type IV
• peptidyl arginine deiminase, type V
• Peptidylarginine deiminase IV
• Protein-arginine deiminase type IV
• protein-arginine deiminase type-4

Background

In vivo expression of PADI4 correlated with the appearance of citrulline in histones. We have shown that PADI4 converts arginines in the histone H3 tail to citrulline, that PADI4 blocks transcriptional activation and is recruited to the endogenous promotor. PADI4 represents a novel class of histone modifying enzymes probably linked to eostrogen regulated transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PADI4