PACSIN2 Antibody - BSA Free

Western Blot: PACSIN2 Antibody [NBP3-06469] - Lane A: Jurkat Whole Cell LysateLane B: NIH/3T3 Whole Cell LysateLane C: RAW 264.7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:55 kDaObserved band size:62 kDa(We are unsure as to the identity of these extra bands.)

Immunohistochemistry-Paraffin: PACSIN2 Antibody [NBP3-06469] - Staining of human PACSIN2 in human kidney with rabbit polyclonal antibody at 1:100 dilution.

Immunoprecipitation: PACSIN2 Antibody [NBP3-06469] - Lane A:0.5 mg NIH/3T3 Whole Cell Lysate4 uL anti-PACSIN2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PACSIN2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 56 kDaObserved band size :60 kDa

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PACSIN2 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human PACSIN2 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PACSIN2
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for PACSIN2 Antibody - BSA Free

• cytoplasmic phosphoprotein PACSIN2
• protein kinase C and casein kinase substrate in neurons 2
• protein kinase C and casein kinase substrate in neurons protein 2
• SDPII
• syndapin II

Background

PACSIN2 may play a role in vesicle formation and transport

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PACSIN2