Recombinant Human TPMT Protein

SDS-Page: Recombinant Human TPMT Protein [NBC1-18525] - TPMT, 28 kDa (245 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human TPMT Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-245 of Human TPMT
  
  

  Source: E.coli

  Amino Acid Sequence: MDGTRTSLDI EEYSDTEVQK NQVLTLEEWQ DKWVNGKTAF HQEQGHQLLK KHLDTFLKGK SGLRVFFPLC GKAVEMKWFA DRGHSVVGVE ISELGIQEFF TEQNLSYSEE PITEIPGTKV FKSSSGNISL YCCSIFDLPR TNIGKFDMIW DRGALVAINP GDRKCYADTM FSLLGKKFQY LLCVLSYDPT KHPGPPFYVP HAEIERLFGK ICNIRRLEKV DAFEERHKSW GIDCLFEKLY LLTEK

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TPMT
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.2 mM PMSF, 2 mM EDTA
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human TPMT Protein

• EC 2.1.1.67
• S-adenosyl-L-methionine:thiopurine S-methyltransferase
• Thiopurine methyltransferase
• thiopurine S-methyltransferase
• TPMT

Background

TPMT, thiopurine S-methyltransferase, is a cytosolic enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine and azathioprine are used as chemotherapeutic agents. TPMT activity exhibits autosomal codominant genetic polymorphism, and patients inheriting TPMT-deficiency are at high risk of potentially fatal hematopoietic toxicity. Recombinant human TPMT was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for TPMT Recombinant Protein (NBC1-18525)(1)

Publication using NBC1-18525

• Maw HH, Zeng X, Campbell S et al. N-methylation of BI 187004 by thiol S-methyltransferase. Drug Metab. Dispos. 2018-03-07 [PMID: 29514826] (Human)

Bioinformatics

• Gene Symbol: TPMT