UBR5/EDD Antibody

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Western Blot: UBR5/EDD Antibody [NB100-1591] - Detection of Human EDD1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Immunohistochemistry-Paraffin: UBR5/EDD Antibody [NB100-1591] - FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-EDD1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.
Western Blot: UBR5/EDD Antibody [NB100-1591] - Whole cell lysate (50 ug) from mouse NIH3T3 and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EDD1 antibody used for WB at 0.4 ...read more
Immunohistochemistry: UBR5/EDD Antibody [NB100-1591] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-EDD1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.
Immunoprecipitation: UBR5/EDD Antibody [NB100-1591] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

UBR5/EDD Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 600 and 650 of human E3 Ubiquitin Protein Ligase, HECTdomain containing, 1 using the numbering given in entry NP_056986.2 (GeneID 51366)
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBR5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-10 mg/mg
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
309 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-1591 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UBR5/EDD Antibody

  • DD5
  • E3 ubiquitin protein ligase, HECT domain containing, 1
  • E3 ubiquitin-protein ligase, HECT domain-containing 1
  • EC 6.3.2
  • EC 6.3.2.-
  • EDD
  • EDD1
  • EDD1E3 ubiquitin-protein ligase UBR5
  • EDDE3 identified by differential display
  • hHYD
  • HYD
  • HYDFLJ11310
  • Hyperplastic discs protein homolog
  • KIAA0896MGC57263
  • progestin induced protein
  • Progestin-induced protein
  • ubiquitin protein ligase E3 component n-recognin 5
  • ubiquitin-protein ligase
  • UBR5

Background

This gene encodes a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22 which is disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation. EDD1 has also been shown to associate with Chk2 and is necessary for the efficient phosphorylation and activation of Chk2 in response to DNA damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for UBR5/EDD Antibody (NB100-1591)(6)

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WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol UBR5
Entrez
Uniprot