UBR5/EDD Antibody

Images

 
Western Blot: UBR5/EDD Antibody [NB100-1591] - Samples: Whole cell lysate (50 ug) from HEK293T, Jurkat, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit UBR5/EDD Antibody (NB100-1591, ...read more
Immunohistochemistry-Paraffin: UBR5/EDD Antibody [NB100-1591] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit UBR5/EDD Antibody (NB100-1591, lot 6) used at a dilution of 1:1,000 (1 ...read more
Immunohistochemistry-Paraffin: UBR5/EDD Antibody [NB100-1591] - Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit UBR5/EDD Antibody (NB100-1591, lot 6) used at a dilution of 1:1000 (1 ...read more
Immunoprecipitation: UBR5/EDD Antibody [NB100-1591] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

UBR5/EDD Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 600 and 650 of human E3 Ubiquitin Protein Ligase, HECTdomain containing, 1 using the numbering given in entry NP_056986.2 (GeneID 51366)
Localization
Nucleus
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBR5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2 - 10 ug/mg of lysate
  • Knockdown Validated
  • Western Blot 1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
309 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-1591 in the following applications:

  • 1 publication
  • KD
    1 publication
  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UBR5/EDD Antibody

  • DD5
  • E3 ubiquitin protein ligase, HECT domain containing, 1
  • E3 ubiquitin-protein ligase, HECT domain-containing 1
  • EC 6.3.2
  • EC 6.3.2.-
  • EDD
  • EDD1
  • EDD1E3 ubiquitin-protein ligase UBR5
  • EDDE3 identified by differential display
  • hHYD
  • HYD
  • HYDFLJ11310
  • Hyperplastic discs protein homolog
  • KIAA0896MGC57263
  • progestin induced protein
  • Progestin-induced protein
  • ubiquitin protein ligase E3 component n-recognin 5
  • ubiquitin-protein ligase
  • UBR5

Background

This gene encodes a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22 which is disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation. EDD1 has also been shown to associate with Chk2 and is necessary for the efficient phosphorylation and activation of Chk2 in response to DNA damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00007337-M01
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, KD, WB
NBP2-01109
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-89150
Species: Hu, Rt
Applications: IHC, IHC-P, WB
AF2574
Species: Hu
Applications: CyTOF-ready, ICFlow
H00023327-P01
Species: Hu
Applications: ELISA, Func, AP, PA, WB
NB100-91662
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP2-31361
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-81988
Species: Hu
Applications: IHC, IHC-P
NBP2-66825
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP2-21037
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-66987
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-27197
Species: Eq, Hu, Mu, Pm, Rt
Applications: WB
NBP1-89522
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
E2-622
Species: Hu
Applications: EnzAct
NBP2-89134
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-13961
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-54301
Species: Hu, Mu, Rt
Applications: CyTOF-ready, ELISA, IHC, IHC-P, WB

Publications for UBR5/EDD Antibody (NB100-1591)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC-P, KD, WB.


Filter By Application
IHC-P
(1)
KD
(1)
WB
(3)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB100-1591 Applications Species
Wang C, Hong T, Wang Y et al. Combining UBR5 and CD163+ tumor-associated macrophages better predicts prognosis of clear cell renal cell carcinoma patients Cancer immunology, immunotherapy : CII Mar 12 2021 [PMID: 33710368]
Bian P, Dou Z, Jia Z et al. Activated Wnt/beta-Catenin signaling contributes to E3 ubiquitin ligase EDD-conferred docetaxel resistance in prostate cancer Life Sci. Aug 28 2019 [PMID: 31472148] (WB, IHC-P, KD, Human, Mouse) WB, IHC-P, KD Human, Mouse
Metzig MO, Fuchs D, Tagscherer KE, Grone HJ. Inhibition of caspases primes colon cancer cells for 5-fluorouracil-induced TNF-a-dependent necroptosis driven by RIP1 kinase and NF-kB. Oncogene. 2015 Nov 02 [PMID: 26522725] (WB, Human) WB Human
Christofferson DE, Li Y, Hitomi J et al. A novel role for RIP1 kinase in mediating TNF? production Cell Death Dis 2012 Jun 14 [PMID: 22695613] (WB, Human) WB Human
Odagiri S, Tanji K, Mori F et al. Immunohistochemical analysis of Marinesco bodies, using antibodies against proteins implicated in the ubiquitin-proteasome system, autophagy and aggresome formation. Neuropathology 2012 Jun [PMID: 22118216]
Dompe N, Rivers CS, Li L et al. A whole-genome RNAi screen identifies an 8q22 gene cluster that inhibits death receptor-mediated apoptosis. Proc Natl Acad Sci U S A 2011 Oct [PMID: 21949371]
Ling S, Lin WC. EDD inhibits ATM-mediated phosphorylation of p53. J Biol Chem 2011 Apr [PMID: 21383020]
O'Brien PM, Davies MJ, Scurry JP et al. The E3 ubiquitin ligase EDD is an adverse prognostic factor for serous epithelial ovarian cancer and modulates cisplatin resistance in vitro. Br J Cancer 2008 Mar [PMID: 18349819]

Reviews for UBR5/EDD Antibody (NB100-1591) (0)

There are no reviews for UBR5/EDD Antibody (NB100-1591). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for UBR5/EDD Antibody (NB100-1591) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional UBR5/EDD Products

Bioinformatics Tool for UBR5/EDD Antibody (NB100-1591)

Discover related pathways, diseases and genes to UBR5/EDD Antibody (NB100-1591). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for UBR5/EDD Antibody (NB100-1591)

Discover more about diseases related to UBR5/EDD Antibody (NB100-1591).
 

Pathways for UBR5/EDD Antibody (NB100-1591)

View related products by pathway.

PTMs for UBR5/EDD Antibody (NB100-1591)

Learn more about PTMs related to UBR5/EDD Antibody (NB100-1591).

Blogs on UBR5/EDD

There are no specific blogs for UBR5/EDD, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our UBR5/EDD Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol UBR5
Entrez
Uniprot