p97/VCP Antibody (Hs-14)

Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (Hs-14) [NB500-455] - Intra Acrosomal Protein Antibody (Hs-14) [NB500-455] - Analysis of VCP in acetone-permeabilized human sperms using monoclonal antibody Hs-14 demonstrates its location to the acrosome. (Normal spermiogram shown).

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF
• Clone: Hs-14
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

p97/VCP Antibody (Hs-14) Summary

• Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
• Specificity: The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa protein previously identified under the general name "intra-acrosomal protein".
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: VCP
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 3-12 ug/ml
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
  • Western Blot 1:1000
• Application Notes: Staining technique: Membrane permeabilization (acetone) is essential. Application note: The antibody Hs-14 is designed for quantitative immunofluorescence analysis of pathological sperms (excellent tool for laboratories of assisted reproduction when optimal method of fertilization is sought).
• Theoretical MW: 220 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS (pH 8.0)
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Multi-step

Alternate Names for p97/VCP Antibody (Hs-14)

• 15S Mg(2+)-ATPase p97 subunit
• IBMPFD
• IBMPFDMGC8560
• MGC131997
• p97
• TER ATPase
• TERA
• transitional endoplasmic reticulum ATPase
• valosin containing protein
• Valosin-containing proteinMGC148092
• VCP
• yeast Cdc48p homolog

Background

This protein is a testis-specific, differentiation antigen, acrosomal vesicle protein 1, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. The gene encoding this protein consists of 4 exons and its alternative splicing generates 11 distinct transcripts, which encode protein isoforms ranging from 81 to 265 amino acids. The longest transcript is the most abundant, comprising 53-72% of the total acrosomal vesicle protein 1 messages; the second largest transcript comprises 15-32%; the third and the fourth largest transcripts account for 3.4-8.3% and 8.7-12.5%, respectively; and the remaining 7 transcripts combined account for < 1% of the total acrosomal vesicle protein 1 message. It is suggested that phenomena of cryptic splicing and exon skipping occur within this gene. The acrosomal vesicle protein 1 may be involved in sperm-zona binding or penetration, and it is a potential contraceptive vaccine immunogen for humans.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p97/VCP Antibody (NB500-455)(2)

Publications using NB500-455

• Elemento JK, Cohen PE Dynamic transcriptome profiles within spermatogonial and 2 spermatocyte populations during postnatal testis maturation 3 revealed by single-cell sequencing PLoS Genet 2019-03-21 [PMID: 30893341] (FLOW, Mouse)
• Greenhalgh JR, Griffith TS, Wald M. The Use of Immunofluorescence in Microdissection Testicular Sperm Extraction J rol 2009-01-01 [PMID: 19234316]
  
  Details:
  This citation used the FITC version of this antibody.

Bioinformatics

• Gene Symbol: VCP
• Entrez:
  • 56(Human)
  • 11451(Mouse)