p97/VCP Antibody (Hs-14) [FITC]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
Hs-14
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

p97/VCP Antibody (Hs-14) [FITC] Summary

Immunogen
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Specificity
The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa protein previously identified under the general name "intra-acrosomal protein".
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
VCP
Purity
Size Exclusion Chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
Publications
Read Publication using NB500-455F.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
TBS and 0.2% (w/v) high grade BSA (protease free)
Preservative
15mM Sodium Azide
Purity
Size Exclusion Chromatography

Alternate Names for p97/VCP Antibody (Hs-14) [FITC]

  • 15S Mg(2+)-ATPase p97 subunit
  • IBMPFD
  • IBMPFDMGC8560
  • MGC131997
  • p97
  • TER ATPase
  • TERA
  • transitional endoplasmic reticulum ATPase
  • valosin containing protein
  • Valosin-containing proteinMGC148092
  • VCP
  • yeast Cdc48p homolog

Background

VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p97/VCP Antibody (NB500-455F)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for p97/VCP Antibody (NB500-455F) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB500-455APC
DyLight 350 Labeled NB500-455UV
DyLight 405 Labeled NB500-455V
DyLight 488 Labeled NB500-455G
DyLight 680 Labeled NB500-455FR
DyLight 755 Labeled NB500-455IR
PE Labeled NB500-455PE
PerCP Labeled NB500-455PCP

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Bioinformatics

Gene Symbol VCP
Entrez