p70 S6 Kinase/S6K [p Ser398] Antibody - Azide Free

Western Blot: p70 S6 Kinase/S6K [p ser 398] Antibody [NBP3-11950] - Western blot of Drosophila S2 cell lysate showing specific labeling of the ~70 kDa p70 S6 Kinase/S6K protein phosphorylated at Thr398 in the first lane (-). Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen in the second lane (+), but not by the corresponding non-phosphopeptide (not shown).

• Reactivity: Dr
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

p70 S6 Kinase/S6K [p Ser398] Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit p70 S6 Kinase/S6K [p Ser398] Antibody - Azide Free (NBP3-11950) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr398 conjugated to KLH. (UniProt: H1ZYE3)
• Modification: p Ser398
• Specificity: Specific for endogenous levels of the ~70 kDa p70 S6 Kinase/S6K protein phosphorylated at Thr398. Immunolabeling is blocked by preadsorption with the Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPS6KB1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: 10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for p70 S6 Kinase/S6K [p Ser398] Antibody - Azide Free

• EC 2.7.11
• EC 2.7.11.1
• p70 ribosomal S6 kinase alpha
• p70 S6 kinase alpha
• p70 S6 Kinase
• p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1
• p70 S6 kinase, alpha 2
• p70 S6KA
• p70 S6K-alpha
• p70(S6K)-alpha
• p70-alpha
• p70-S6K
• P70S6K1
• PS6K
• ribosomal protein S6 kinase beta-1
• Ribosomal protein S6 kinase I
• ribosomal protein S6 kinase, 70kD, polypeptide 1
• ribosomal protein S6 kinase, 70kDa, polypeptide 1
• RPS6KB1
• S6K
• S6K1
• S6K1p70-S6K 1
• S6K-beta-1
• serine/threonine kinase 14 alpha
• Serine/threonine-protein kinase 14A
• STK14A

Background

S6K encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPS6KB1