RPS6KB1 Products By Gene Name

HAF008). A specific band was detected for p70 S6 Kinase at approximately 70 and 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF008). A specific band was detected for p70 S6 Kinase at approximately 70 and 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human p70 S6 Kinase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8962) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for p70 S6 Kinase at approximately 70 and 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (F0110). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol." class="big_lightbox"> AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (F0110). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol." class="big_thumb" />

CTS005) and counterstained with hematoxylin (blue). Specific labeling was localized to the nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS005) and counterstained with hematoxylin (blue). Specific labeling was localized to the nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

291-G1) for 20 minutes and NIH-3T3 mouse embryonic fibroblast cell line untreated or treated with 100 ng/mL of Human PDGF (Catalog # 120-HD) for 20 minutes. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-p70 S6 Kinase (T421/S424) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8965), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-p70 S6 Kinase (T421/S424) at approximately 70 and 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> 291-G1) for 20 minutes and NIH-3T3 mouse embryonic fibroblast cell line untreated or treated with 100 ng/mL of Human PDGF (Catalog # 120-HD) for 20 minutes. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-p70 S6 Kinase (T421/S424) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8965), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-p70 S6 Kinase (T421/S424) at approximately 70 and 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL of Recombinant Human IGF-1 (Catalog # 291-G1) for 20 minutes and NIH-3T3 mouse embryonic fibroblast cell line untreated or treated with 100 ng/mL of Human PDGF (Catalog # 120-HD) for 20 minutes. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-p70 S6 Kinase (T421/S424) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8965), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-p70 S6 Kinase (T421/S424) at approximately 70 and 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

291-G1) for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-p70 S6 Kinase (T421/S424) at approximately 66 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-p70 S6 Kinase (T421/S424) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8965). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox"> 291-G1) for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-p70 S6 Kinase (T421/S424) at approximately 66 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-p70 S6 Kinase (T421/S424) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8965). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

291-G1) for 60 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-p70 S6 Kinase (T389) Monoclonal Antibody (Catalog # MAB8963) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-p70 S6 Kinase (T389) and Phospho-p85 S6 Kinase (T412) at approximately 70 and 85 kDa, respectively (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> 291-G1) for 60 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-p70 S6 Kinase (T389) Monoclonal Antibody (Catalog # MAB8963) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-p70 S6 Kinase (T389) and Phospho-p85 S6 Kinase (T412) at approximately 70 and 85 kDa, respectively (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1) for 60 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-p70 S6 Kinase (T389) Monoclonal Antibody (Catalog # MAB8963) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-p70 S6 Kinase (T389) and Phospho-p85 S6 Kinase (T412) at approximately 70 and 85 kDa, respectively (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

291-G1) using Rabbit Anti-Human Phospho-p70 S6 Kinase (T389) Monoclonal Antibody (Catalog # MAB8963) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> 291-G1) using Rabbit Anti-Human Phospho-p70 S6 Kinase (T389) Monoclonal Antibody (Catalog # MAB8963) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol." class="big_lightbox"> MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol." alt="HeLa human cervical epithelial carcinoma cell line was stained with Human/Mouse/Rat p70 S6 Kinase Monoclonal Antibody (Catalog # MAB8962, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol." class="big_thumb" />

HAF007). For additional reference, Recombinant Human Active p70 S6 Kinase (Catalog # 896-KS) was included. A specific band for p70 S6 Kinase was detected at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF007). For additional reference, Recombinant Human Active p70 S6 Kinase (Catalog # 896-KS) was included. A specific band for p70 S6 Kinase was detected at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

291-G1) for 1 hour. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-p70 S6 Kinase (T229) Monoclonal Antibody (Catalog # MAB8964) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-p70 S6 Kinase (T229) at approximately 70 and 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> 291-G1) for 1 hour. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-p70 S6 Kinase (T229) Monoclonal Antibody (Catalog # MAB8964) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-p70 S6 Kinase (T229) at approximately 70 and 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1) for 1 hour. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-p70 S6 Kinase (T229) Monoclonal Antibody (Catalog # MAB8964) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-p70 S6 Kinase (T229) at approximately 70 and 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

291-G1) using Rabbit Anti-Human Phospho-p70 S6 Kinase (T229) Monoclonal Antibody (Catalog # MAB8964) at 2.5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> 291-G1) using Rabbit Anti-Human Phospho-p70 S6 Kinase (T229) Monoclonal Antibody (Catalog # MAB8964) at 2.5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />