p66 alpha Antibody - BSA Free

Western Blot: p66 alpha Antibody [NBP1-87360] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry/Immunofluorescence: p66 alpha Antibody [NBP1-87360] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: p66 alpha Antibody [NBP1-87360] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.

Western Blot: p66 alpha Antibody [NBP1-87360] - Analysis using Anti-GATAD2A antibody NBP1-87360 (A) shows similar pattern to independent antibody NBP1-87359 (B).

Immunohistochemistry-Paraffin: p66 alpha Antibody [NBP1-87360] - Staining of human colon, lymph node, placenta and testis using Anti-GATAD2A antibody NBP1-87360 (A) shows similar protein distribution across tissues to independent antibody NBP1-87359 (B).

Immunohistochemistry-Paraffin: p66 alpha Antibody [NBP1-87360] - Staining of human placenta.

Immunohistochemistry-Paraffin: p66 alpha Antibody [NBP1-87360] - Staining of human colon.

Immunohistochemistry-Paraffin: p66 alpha Antibody [NBP1-87360] - Staining of human testis.

Immunohistochemistry-Paraffin: p66 alpha Antibody [NBP1-87360] - Staining of human lymph node.

Immunohistochemistry-Paraffin: p66 alpha Antibody [NBP1-87360] - Staining of human colon shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: p66 alpha Antibody [NBP1-87360] - Staining of human colon, placenta, skeletal muscle and testis using Anti-p66 alpha antibody NBP1-87360 (A) shows similar protein distribution across tissues to independent antibody NBP1-87359 (B).

Immunohistochemistry-Paraffin: p66 alpha Antibody [NBP1-87360] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: p66 alpha Antibody [NBP1-87360] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.

ChIP-Exo-Seq composite graph for Anti-GATAD2A (NBP1-87360) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, ChIP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

p66 alpha Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit p66 alpha Antibody - BSA Free (NBP1-87360) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LERDPTEDDVESKKIKMERGLLASDLNTDGDMRVTPEPGAGPTQGLLRATEATAMAMGRGEGLVGDGPVDMRTSHSDMKSERRPPSPDVIVLSDNEQPSSPRVNGLTTVALKETSTE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GATAD2A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation-exo-Seq
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  p66 alpha Protein (NBP1-87360PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for p66 alpha Antibody - BSA Free

• FLJ20085
• FLJ21017
• GATA zinc finger domain containing 2A
• GATA zinc finger domain-containing protein 2A
• hp66alpha
• p66 alpha
• p66alpha
• transcriptional repressor p66-alpha

Background

Methylation of cytosine at CpG dinucleotides is a common feature of many higher eukaryotic genomes (1-3). Increasing evidence indicates that recruitement of histone deacetylase complexes by methyl-CpG-binding proteins (MeCP) is a major mechanism of methylated DNA silencing. P66, a zinc finger-containing protein is one of the components of the MeCP1 complex (4). P66 and p68, the two components of MeCP1 are different forms of the same zinc finger-containing protein and are conserved from C.elegans to humans. Homologs of p66 from different organisms revealed two highly conserved regions, CR1 and CR2. While CR1 is involved in the association of p66 with other MeCP1 components, CR2 plays an important role in targeting p66 and MBD3 to specific loci (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GATAD2A