p66 alpha Antibody - BSA Free

Western Blot: p66 alpha Antibody [NB100-56643] - analysis of p66 in 15 ugs of HeLa lysate using p66 antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: p66 alpha Antibody [NB100-56643] - analysis of human tonsil using p66 antibody at 1:50.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

p66 alpha Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a synthetic peptide corresponding to aa 137-150 of mouse p66a. It cross-reacts with human p66a (AAH19178.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GATAD2A
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500-1:1000
• Application Notes: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for p66 alpha Antibody - BSA Free

• FLJ20085
• FLJ21017
• GATA zinc finger domain containing 2A
• GATA zinc finger domain-containing protein 2A
• hp66alpha
• p66 alpha
• p66alpha
• transcriptional repressor p66-alpha

Background

Methylation of cytosine at CpG dinucleotides is a common feature of many higher eukaryotic genomes. Increasing evidence indicates that recruitement of histone deacetylase complexes by methyl-CpG-binding proteins (MeCP) is a major mechanism of methylated DNA silencing. P66, a zinc finger-containing protein is one of the components of the MeCP1 complex. P66 and p68, the two components of MeCP1 are different forms of the same zinc finger-containing protein and are conserved from C.elegans to humans. Homologs of p66 from different organisms revealed two highly conserved regions, CR1 and CR2. While CR1 is involved in the association of p66 with other MeCP1 components, CR2 plays an important role in targeting p66 and MBD3 to specific loci.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p66 alpha Antibody (NB100-56643)(1)

Publication using NB100-56643

Bioinformatics

• Gene Symbol: GATAD2A
• Entrez:
  • 54815(Human)
  • 234366(Mouse)
• Uniprot:
  • Q8CHY6(Mouse)