p63/TP73L Antibody - BSA Free

Product Discontinued

p63/TP73L Antibody - BSA Free Summary

• Immunogen: A synthetic peptide (ENNAQTQFSEPQY) corresponding to aa5-17 of human p40
• Localization: Nuclear
• Marker: Squamous, Basal & Myoepithelial Cell Marker
• Specificity: p40 (p63 delta) is a marker recently determined to be highly specific for squamous basal cells in the immunohistochemistry (IHC) application. The current more routinely recommended marker, p63, appears to have less specificity compared to p40, especially on squamous cell tumors. The ability to differentiate between lung adenocarcinoma vs. squamous cell carcinoma is difficult and has bearing on the different therapeutic avenues for each subtype treatment. p63 antibody's ability to distinguish between the tumor types appears to be inferior when compared to p40. The ability to utilize an antibody probe for p40 as a squamous cell marker bolsters its use for future sub-classification of lung cancers, especially by immunohistochemical techniques.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TP63
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2ug/ml
  • Immunohistochemistry 0.5-1ug/ml
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 0.5-1ug/500ug protein lysate
  • Western Blot 0.5-1ug/ml
• Application Notes: Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 29513607). This antibody is CyTOF ready.
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for p63/TP73L Antibody - BSA Free

• amplified in squamous cell carcinoma
• Chronic ulcerative stomatitis protein
• CUSP
• EEC3
• EEC3B(p51B)
• Keratinocyte transcription factor KET
• KET
• KETRHS
• LMS
• OFC8
• OFC8SHFM4TP73LNBP
• p40
• p51
• p51tumor protein p53-like
• p53CP
• p63
• p63AIS
• p73H
• p73L
• p73LB(p51A)
• SHFM4
• TP53CP
• TP53L
• TP63
• TP73L
• Transformation-related protein 63
• tumor protein 63
• tumor protein p53-competing protein
• tumor protein p63 deltaN isoform delta
• tumor protein p63
• Tumor protein p73-like

Background

The p63 gene, a homologue of the tumor suppressor p53, is highly expressed in the basal or progenitor layers of many epithelial tissues. P63 shows remarkable structural similarity to p53 and to the related p73 gene. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p63/TP73L Antibody (NBP2-33090)(3)

Publications using NBP2-33090

• Rostampour N, Appelt CM, Abid A, Boughner JC. Expression of new genes in vertebrate tooth development and p63 signaling Dev. Dyn. 2019-03-15 [PMID: 30875130] (IHC-P, Mouse)
• Bojic, S, Hallam, D Et al. CD200 Expression Marks a Population of Quiescent Limbal Epithelial Stem Cells with Holoclone Forming Ability. Stem Cells 2018-11-01 [PMID: 30157305] (WB, Human)
• Hayano S, Fukui Y, Kawanabe N et al. Role of the Inferior Alveolar Nerve in Rodent Lower Incisor Stem Cells. J. Dent. Res. 2018-03-01 [PMID: 29513607] (IHC-Fr, Rat)

Bioinformatics

• Gene Symbol: TP63