p63/TP73L Antibody (4A4)

Product Discontinued

p63/TP73L Antibody (4A4) Summary

• Immunogen: Recombinant full length protein, corresponding to amino acids 1-205 of human p63.
• Localization: Nuclear
• Specificity: By immunoblotting, 3 bands are visible at approx. 50, 60, and 70 kDa using HaCaT cells, and multiple bands of approx. 44, 60, 80, and 90-95 kDa in other preparations.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TP63
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:75
  • Western Blot
• Application Notes: Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of 63 kDa, which corresponds to the predicted molecular weight of p63. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues. Use in Western blot reported in scientific literature (PMID 25988972)

Reactivity Notes

Cross-reacts with Human. Mouse reactivity reported in scientific literature (PMID: 25988972)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS with albumin and small amount of non - ionic detergent
• Preservative: 0.05% Sodium Azide
• Concentration: 2.0 mg/ml
• Purity: Protein A purified

Alternate Names for p63/TP73L Antibody (4A4)

• amplified in squamous cell carcinoma
• Chronic ulcerative stomatitis protein
• CUSP
• EEC3
• EEC3B(p51B)
• Keratinocyte transcription factor KET
• KET
• KETRHS
• LMS
• OFC8
• OFC8SHFM4TP73LNBP
• p40
• p51
• p51tumor protein p53-like
• p53CP
• p63
• p63AIS
• p73H
• p73L
• p73LB(p51A)
• SHFM4
• TP53CP
• TP53L
• TP63
• TP73L
• Transformation-related protein 63
• tumor protein 63
• tumor protein p53-competing protein
• tumor protein p63 deltaN isoform delta
• tumor protein p63
• Tumor protein p73-like

Background

This gene encodes a member of the p53 family of transcription factors. An animal model, p63 -/- mice, has been useful in defining the role this protein plays in the development and maintenance of stratified epithelial tissues. p63 -/- mice have several developmental defects which include the lack of limbs and other tissues, such as teeth and mammary glands, which develop as a result of interactions between mesenchyme and epithelium. Mutations in this gene are associated with ectodermal dysplasia, and cleft lip/palate syndrome 3 (EEC3); split-hand/foot malformation 4 (SHFM4); ankyloblepharon-ectodermal defects-cleft lip/palate; ADULT syndrome (acro-dermato-ungual-lacrimal-tooth); limb-mammary syndrome; Rap-Hodgkin syndrome (RHS); and orofacial cleft 8. Both alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different proteins. Many transcripts encoding different proteins have been reported but the biological validity and the full-length nature of these variants have not been determined

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p63/TP73L Antibody (NB100-691)(13)

Publications using NB100-691

• Jakobsson L, Chebil G, Marzouka NA et al. Low Frequency of Intratumor Heterogeneity in Bladder Cancer Tissue Microarrays Bladder Cancer 2018-07-30 [PMID: 30112444] (IF/IHC, Human)
• Sjodahl G. Molecular Subtype Profiling of Urothelial Carcinoma Using a Subtype-Specific Immunohistochemistry Panel Methods Mol. Biol. 2017-09-10 [PMID: 28889377] (IF/IHC)
• Rinaldi VD, Bolcun-Filas E, Kogo H et al. The DNA Damage Checkpoint Eliminates Mouse Oocytes with Chromosome Synapsis Failure Mol. Cell 2017-08-16 [PMID: 28844861] (Mouse)
• Kuramochi Mizuki, Izawa Takeshi, Nishimura Shin et al. Pleomorphic adenoma of the labial gland, characterized by reticular pattern of myoepithelial cells in a dog. The Journal of Veterinary Medical Science 2017-06-07 [PMID: 28529274] (IF/IHC, Canine)
• Haffner MC, Weier C, Xu M et al. Molecular evidence that invasive adenocarcinoma can mimic prostatic intraepithelial neoplasia (PIN) and intraductal carcinoma through retrograde glandular colonization. J. Pathol. 2015-08-31 [PMID: 26331372] (IHC-P, Human)
  
  Details:
  p63/TP73L antibody (clone 4A4) was used for IHC-P on human tissue sections from high-grade prostatic intraepithelial neoplasia (HGPIN) patients.
• Palla AR, Piazzolla D, Alcazar N et al. The pluripotency factor NANOG promotes the formation of squamous cell carcinomas. Sci Rep 2015-01-01 [PMID: 25988972] (WB, Mouse)
• De Las Heras M, de Martino A, Borobia M et al. Solitary Tumours Associated with Jaagsiekte Retrovirus in Sheep are Heterogeneous and Contain Cells Expressing Markers Identifying Progenitor Cells in Lung Repair. J Comp Pathol. 2013-10-29 [PMID: 24176105] (IF/IHC, Sheep)
• Lotan TL, Gumuskaya B, Rahimi H et al. Cytoplasmic PTEN protein loss distinguishes intraductal carcinoma of the prostate from high-grade prostatic intraepithelial neoplasia Mod Pathol 2012-12-07 [PMID: 23222491] (IF/IHC, Human)
• Yang A, Kaghad M, Wang Y et al. p63, a p53 homolog at 3q27-29, encodes multiple products with transactivating, death-inducing, and dominant-negative activities. Mol Cell. 1998-09-01 [PMID: 9774969] (WB, IHC-P)
  
  Details:
  IHC (P), Fig 1: human epithelial tissues; WB: Fig 5, primary human foreskin keratinocytes, ME180 human cervical carcinoma cells, and BHK cells expressing p63 isotypes. Note: This is the original (first) 4A4 clone citation. The 4A4 antibody was shown to recognize all p63 isotypes by western blot (Fig 5).
• Hastings JF, Gonzalez-Rajal A, Han JZR, McCloy RA Dynamic analysis of pulsed cisplatin identifies effectors of resistance in lung adenocarcinoma bioRxiv
• Holliday H, Roden DL, Junankar S et al. ID4 represses myoepithelial differentiation of mammary stem cells through its interaction with HEB iScience 2021-02-08 [PMID: 33554073]
• Hastings JF, Gonzalez Rajal A, Latham SL et al. Analysis of pulsed cisplatin signalling dynamics identifies effectors of resistance in lung adenocarcinoma Elife 2020-06-09 [PMID: 32513387] (WB, Human)
• Dodd ME, Hatzold J, Mathias JR et al. The ENTH domain protein Clint1 is required for epidermal homeostasis in zebrafish. Development;136(15):2591-2600. 2009-01-01 [PMID: 19570844]

Bioinformatics

• Gene Symbol: TP63
• Entrez:
  • 8626(Human)
  • 22061(Mouse)
  • 246334(Rat)
• Uniprot:
  • Q9H3D4()