p53R2 Antibody

Images

 
Western Blot: p53R2 Antibody [NBP1-76781] - Analysis of p53R2 in 3T3 cell lysate with p53R2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: p53R2 Antibody [NBP1-76781] - Immunofluorescence of p53R2 in Human Lung tissue with p53R2 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: p53R2 Antibody [NBP1-76781] - Human lung tissue with p53R2 antibody at 1 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

p53R2 Antibody Summary

Immunogen
Antibody was raised against a 16 amino acid peptide near the amino terminus of human p53R2. The immunogen is located within the first 50 amino acids of p53R2. Amino Acid Squence: GDPERPEAAGLDQDER
Specificity
At least six isoforms of p53R2 are known to exist; this antibody will detect all but the largest isoform.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RRM2B
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1 ug/ml
  • Immunohistochemistry-Paraffin 1 ug/ml
Application Notes
This antibody can be used for detection of p53R2 by Western blot at 0.5 to 1 mg/ml. A 39 kDa band can be detected.
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
p53R2 Knockout HeLa Cell Lysate
Control Peptide
p53R2 Peptide (NBP1-76781PEP)
Publications
Read Publication using
NBP1-76781 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for p53R2 Antibody

  • EC 1.17.4.1
  • MGC102856
  • MGC42116
  • MTDPS8A
  • MTDPS8B
  • p53-inducible ribonucleotide reductase small subunit 2 homolog
  • p53-inducible ribonucleotide reductase small subunit 2-like protein
  • p53R2
  • P53R2DKFZp686M05248
  • PEOA5
  • ribonucleoside-diphosphate reductase subunit M2 B
  • ribonucleotide reductase M2 B (TP53 inducible)
  • RRM2B
  • TP53-inducible ribonucleotide reductase M2 B

Background

The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including p53R2, Chk2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. p53R2 is a p53 inducible gene that contains a p53 binding sequence and encodes a subunit of the enzyme ribonucleotide reductase. p53R2 is induced by the reagents, ultraviolet and gamma-irradiation that cause DNA damages. The product of p53R2 gene is directly involved in the p53 checkpoint for repair of damaged DNA. The isoform of the p53 family member p73 also induces p53R2 expression. p53R2 is an important target of p53 for tumour suppression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p53R2 Antibody (NBP1-76781)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


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IHC
(1)
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Filter By Species
Human
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol RRM2B
Uniprot