Home » p53R2 » p53R2 Antibodies » p53R2 Antibody - BSA Free

p53R2 Antibody - BSA Free

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Western Blot: p53R2 Antibody [NBP1-76781] - Analysis of p53R2 in 3T3 cell lysate with p53R2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Western Blot: p53R2 Antibody [NBP1-76781] - Analysis of p53R2 in 3T3 cell lysate with p53R2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: p53R2 Antibody [NBP1-76781] - Immunofluorescence of p53R2 in Human Lung tissue with p53R2 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: p53R2 Antibody [NBP1-76781] - Human lung tissue with p53R2 antibody at 1 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-76781AF350
Alexa Fluor 405
NBP1-76781AF405
Alexa Fluor 488
NBP1-76781AF488
Alexa Fluor 532
NBP1-76781AF532
Alexa Fluor 594
NBP1-76781AF594
Alexa Fluor 647
NBP1-76781AF647
Alexa Fluor 700
NBP1-76781AF700
Alexa Fluor 750
NBP1-76781AF750
Biotin
NBP1-76781B
DyLight 350
NBP1-76781UV
DyLight 405
NBP1-76781V
DyLight 488
NBP1-76781G
DyLight 550
NBP1-76781R
DyLight 594
NBP1-76781DL594
DyLight 650
NBP1-76781C
DyLight 680
NBP1-76781FR
DyLight 755
NBP1-76781IR
FITC
NBP1-76781F
HRP
NBP1-76781H
Janelia Fluor 549
NBP1-76781JF549
Janelia Fluor 646
NBP1-76781JF646
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-76781-0.025mg
100% Guarantee on every product

p53R2 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 16 amino acid peptide near the amino terminus of human p53R2. The immunogen is located within the first 50 amino acids of p53R2. Amino Acid Squence: GDPERPEAAGLDQDER
Specificity
At least six isoforms of p53R2 are known to exist; this antibody will detect all but the largest isoform.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RRM2B
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1 ug/ml
  • Immunohistochemistry-Paraffin 1 ug/ml
  • Western Blot 1 ug/ml
Application Notes
This antibody can be used for detection of p53R2 by Western blot at 0.5 to 1 mg/ml. A 39 kDa band can be detected.
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
p53R2 Peptide (NBP1-76781PEP)
Publications
Read Publication using
NBP1-76781 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for p53R2 Antibody - BSA Free

  • EC 1.17.4.1
  • MGC102856
  • MGC42116
  • MTDPS8A
  • MTDPS8B
  • p53-inducible ribonucleotide reductase small subunit 2 homolog
  • p53-inducible ribonucleotide reductase small subunit 2-like protein
  • p53R2
  • P53R2DKFZp686M05248
  • PEOA5
  • ribonucleoside-diphosphate reductase subunit M2 B
  • ribonucleotide reductase M2 B (TP53 inducible)
  • RRM2B
  • TP53-inducible ribonucleotide reductase M2 B

Background

The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including p53R2 , Chk2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. p53R2 is a p53 inducible gene that contains a p53 binding sequence and encodes a subunit of the enzyme ribonucleotide reductase. p53R2 is induced by the reagents, ultraviolet and gamma-irradiation that cause DNA damages. The product of p53R2 gene is directly involved in the p53 checkpoint for repair of damaged DNA. The isoform of the p53 family member p73 also induces p53R2 expression. p53R2 is an important target of p53 for tumour suppression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on A549 cells with p53 (PAb240) NB200-103AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow (Intracellular): p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by APC-conjugated anti-mouse IgG secondary antibody F0101B.
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Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] - Rat intestine. RRM2 antibody [N1C1] iluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Western Blot: RRM1 Antibody [NBP2-20231] - Sample (30 ug of whole cell lysate) A: 293T 5% SDS PAGE gel, diluted at 1:2000.
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(SZ25-01)
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Flow Cytometry: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of Hela cells with ERK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
(1 Review)
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p53R2 Antibody -
BSA Free
NBP1-76781
p53R2 Antibody - BSA Free
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

Publications for p53R2 Antibody (NBP1-76781)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


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Bioinformatics

Gene Symbol RRM2B
Uniprot