Human P-Selectin/CD62P ELISA Kit (Colorimetric)

ELISA: Human P-Selectin/CD62P ELISA Kit (Colorimetric) [NBP1-89873] - Representative standard curve for human P-selectin ELISA. Human P-selectin was diluted in serial 2-fold steps in Sample Diluent . Do not use this standard curve to derive test results. A standard curve must be run for each group of microwell strips assayed.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), amniotic fluid
• Standard Curve Range: 0.63 - 40.00 ng/mL
• Sensitivity: 0.20 ng/mL

Human P-Selectin/CD62P ELISA Kit (Colorimetric) Summary

• Description: This kit is an enzyme-linked immunosorbent assay for quantitative detection.
• Specificity: The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human P-selectin positive sample. There was no cross reactivity or interference detected, notably not with human E-selectin and human L-selectin.
• Standard Curve Range: 0.63 - 40.00 ng/mL
• Sensitivity: 0.20 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 5.4%
• Intra-Assay: CV% < 7.8%
• Spike Recovery: For recovery data see the manual.
• Sample Volume: 10 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: SELP

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human P-Selectin/CD62P ELISA Kit (Colorimetric)

• CD62P antigen
• CD62P
• FLJ45155
• GMP140
• GRMP
• PADGEM
• PADGEMantigen CD62)
• PSEL
• P-Selectin
• selectin P (granule membrane protein 140kDa, antigen CD62)
• SELP

Background

P-selectin belongs to the selectin family of adhesion molecules. P-selectin acts as a receptor that supports binding of leukocytes to activated platelets and endothelium. P-selectin is a 140 kDa protein that is highly glycosylated. The cDNA derived amino acid sequence predicts a molecule with a series of cysteine-rich domains. P-selectin is located in membranes of a granules in unstimulated platelets and redistributed to the cell surface upon platelet activation. P-selectin is also present in endothelial cells in membranes of Weibel-Palade bodies and megakaryocytes. The physiologic role of P-selectin might be the mediation of initial leukocyte adhesion to activated endothelium during acute inflammation. It may work in concert with E-selectin to direct early, regionally specific adherence of neutrophils and monocytes at sites of acute inflammation. A soluble form of P-selectin found in serum and plasma has been described which might represent a proteolytic fragment or more likely a soluble splice variant lacking the transmembrane domain. Excessive accumulation of neutrophils on the endothelial surface accompanied by high exposure of P-selectin has been implicated in a number of inflammatory disorders, including adult respiratory distress syndrome, acute lung injury, ischemia-reperfusion injury, Gram-negative septic shock, thrombotic diseases and rheumatoid arthritis. Malignant cells were shown to express receptors for P-selectin suggesting an important role for P-selectin in tumor formation and metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: SELP