Human P-Selectin/CD62P ELISA Kit (Colorimetric)

Images

 
ELISA: Human P-Selectin/CD62P ELISA Kit (Colorimetric) [NBP1-89873] - Representative standard curve for human P-selectin ELISA. Human P-selectin was diluted in serial 2-fold steps in Sample Diluent. Do not use this ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Standard Curve Range
0.63 - 40.00 ng/mL
Sensitivity
0.20 ng/mL

Order Details

Human P-Selectin/CD62P ELISA Kit (Colorimetric) Summary

Specificity
The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human P-selectin positive sample. There was no cross reactivity or interference detected, notably not with human E-selectin and human L-selectin.
Standard Curve Range
0.63 - 40.00 ng/mL
Sensitivity
0.20 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
5.4%
Intra-Assay
7.8%
Spike Recovery
For recovery data see the manual.
Sample Volume
10 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
SELP

Applications/Dilutions

Application Notes
This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to GLP-1

Alternate Names for Human P-Selectin/CD62P ELISA Kit (Colorimetric)

  • CD62P antigen
  • CD62P
  • FLJ45155
  • GMP140
  • GRMP
  • PADGEM
  • PADGEMantigen CD62)
  • PSEL
  • P-Selectin
  • selectin P (granule membrane protein 140kDa, antigen CD62)
  • SELP

Background

P-selectin belongs to the selectin family of adhesion molecules. P-selectin acts as a receptor that supports binding of leukocytes to activated platelets and endothelium. P-selectin is a 140 kDa protein that is highly glycosylated. The cDNA derived amino acid sequence predicts a molecule with a series of cysteine-rich domains. P-selectin is located in membranes of a granules in unstimulated platelets and redistributed to the cell surface upon platelet activation. P-selectin is also present in endothelial cells in membranes of Weibel-Palade bodies and megakaryocytes. The physiologic role of P-selectin might be the mediation of initial leukocyte adhesion to activated endothelium during acute inflammation. It may work in concert with E-selectin to direct early, regionally specific adherence of neutrophils and monocytes at sites of acute inflammation. A soluble form of P-selectin found in serum and plasma has been described which might represent a proteolytic fragment or more likely a soluble splice variant lacking the transmembrane domain. Excessive accumulation of neutrophils on the endothelial surface accompanied by high exposure of P-selectin has been implicated in a number of inflammatory disorders, including adult respiratory distress syndrome, acute lung injury, ischemia-reperfusion injury, Gram-negative septic shock, thrombotic diseases and rheumatoid arthritis. Malignant cells were shown to express receptors for P-selectin suggesting an important role for P-selectin in tumor formation and metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-42795
Species: Hu
Applications: WB, Flow, Func, IHC, IHC-Fr, IP, CyTOF-ready
NB600-586
Species: Hu
Applications: ICC/IF, IHC, IHC-P
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
H00003117-M01
Species: Hu
Applications: WB, ELISA, PLA
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
NBP2-42225
Species: Hu, Ca
Applications: WB, DB, EM, ELISA, Flow, Func, ICC/IF, IP
NBP2-37384
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P
NB200-610
Species: Hu, Ca, GP
Applications: Flow, ICC/IF, IHC, IHC-Fr, IP
NB100-78039
Species: Hu, Mu
Applications: WB, ELISA, Flow, IHC, IHC-Fr, IHC-P, CyTOF-ready, Flow-CS
NB110-89474
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-14934
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-1642
Species: Mu
Applications: Flow, ICC/IF, IHC, IHC-Fr, IP, Flow-CS
NBP2-15139
Species: Hu
Applications: WB, IHC, IHC-P

Publications for P-Selectin/CD62P ELISA Kit (NBP1-89873) (0)

There are no publications for P-Selectin/CD62P ELISA Kit (NBP1-89873).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for P-Selectin/CD62P ELISA Kit (NBP1-89873) (0)

There are no reviews for P-Selectin/CD62P ELISA Kit (NBP1-89873). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for P-Selectin/CD62P ELISA Kit (NBP1-89873) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional P-Selectin/CD62P Products

Bioinformatics Tool for P-Selectin/CD62P ELISA Kit (NBP1-89873)

Discover related pathways, diseases and genes to P-Selectin/CD62P ELISA Kit (NBP1-89873). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for P-Selectin/CD62P ELISA Kit (NBP1-89873)

Discover more about diseases related to P-Selectin/CD62P ELISA Kit (NBP1-89873).
 

Pathways for P-Selectin/CD62P ELISA Kit (NBP1-89873)

View related products by pathway.

PTMs for P-Selectin/CD62P ELISA Kit (NBP1-89873)

Learn more about PTMs related to P-Selectin/CD62P ELISA Kit (NBP1-89873).

Blogs on P-Selectin/CD62P

There are no specific blogs for P-Selectin/CD62P, but you can read our latest blog posts.

Customers Who Bought This Also Bought

P-Selectin/CD62P
137-PS-050

Contact Information

Product PDFs

Review this Product

Be the first to review our Human P-Selectin/CD62P ELISA Kit (Colorimetric) and receive a gift card or discount.

Bioinformatics

Gene Symbol SELP
Entrez