Human P-Selectin/CD62P Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human P-Selectin/CD62P Quantikine ELISA Kit Summary

• Background: The Quantikine^® Human P-Selectin/CD62P Immunoassay is a 1.25 hour solid-phase ELISA designed to measure human P-Selectin in cell culture supernates, serum, and plasma. It contains recombinant human P-Selectin and antibodies raised against the recombinant factor. Results obtained using natural human P-Selectin showed linear curves that were parallel to the standard curves obtained... using the Quantikine^® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human P-Selectin.
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• Specificity: Rrecombinant and natural human P-Selectin
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human P-Selectin/CD62P Quantikine ELISA Kit

• CD62P antigen
• CD62P
• FLJ45155
• GMP140
• GRMP
• PADGEM
• PADGEMantigen CD62)
• PSEL
• P-Selectin
• selectin P (granule membrane protein 140kDa, antigen CD62)
• SELP

Background

The Selectin family is comprised of three members, E-Selectin, L-Selectin, and P-Selectin. E-Selectin [endothelial leukocyte adhesion molecule-1 (ELAM-1), CD62E] is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha. The human and rat proteins share approximately 67% amino acid sequence identity. The mouse and rat proteins share approximately 78% amino acid sequence identity. L-Selectin (Leukocyte Selectin, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L) is expressed constitutively on a wide variety of leukocytes. Two forms of L-Selectin have been reported, apparently arising as a result of post-translational modifications. Human and mouse L-Selectin share 76% amino acid sequence identity. Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P) is expressed by activated platelets and endothelial cells. The extracellular domains of human and mouse P-Selectin share approximately 73% amino acid sequence identity.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for P-Selectin/CD62P (DPSE00)(18)

Publications using DPSE00

• Guo, CY;Mo, R;Kim, H; Surface topography modulates initial platelet adhesion to titanium substrata Journal of oral biology and craniofacial research 2024-06-07 [PMID: 38962718] (Human)
• Illési, Á;Fejes, Z;Pócsi, M;Debreceni, IB;Hodosi, K;Nagy, B;Kappelmayer, J;K?szegi, Z;Csanádi, Z;Szük, T; Technically Challenging Percutaneous Interventions of Chronic Total Occlusions Are Associated with Enhanced Platelet Activation Journal of clinical medicine 2023-10-29 [PMID: 37959293]
• Z?bczyk, M;Kruk, A;Natorska, J;Undas, A; Low-grade endotoxemia in acute pulmonary embolism: Links with prothrombotic plasma fibrin clot phenotype Thrombosis research 2023-12-01 [PMID: 37949000] (Human)
• Sommer, P;Schreinlechner, M;Noflatscher, M;Lener, D;Mair, F;Theurl, M;Kirchmair, R;Marschang, P; Increasing Soluble P-Selectin Levels Predict Higher Peripheral Atherosclerotic Plaque Progression Journal of clinical medicine 2023-10-10 [PMID: 37892568] (Human)
• Chyrchel, B;Kruszelnicka, O;Wieczorek-Surdacka, E;Surdacki, A; Association of ADP-Induced Whole-Blood Platelet Aggregation with Serum Low-Density Lipoprotein Cholesterol in Patients with Coronary Artery Disease When Receiving Maintenance Ticagrelor-Based Dual Antiplatelet Therapy Journal of clinical medicine 2023-07-06 [PMID: 37445565] (Human)
• Y Harada, A Sato, A Nishioka, S Ogusu, M Matsumoto, E Sueoka, A Kawaguchi, S Kimura, N Sueoka-Ara Usefulness of blood biomarkers for predicting venous thromboembolism in Japanese patients with cancer Oncology Letters, 2023-03-17;25(5):180. 2023-03-17 [PMID: 37033099] (Human)
• D Mehic, J Machacek, T Schramm, L Buresch, A Kaider, B Eichelberg, H Haslacher, M Fillitz, B Dixer, T Flasch, T Anderle, A Rath, A Assinger, C Ay, I Pabinger, J Gebhart Platelet function and soluble P-selectin in patients with primary immune thrombocytopenia Thrombosis Research, 2023-01-16;223(0):102-110. 2023-01-16 [PMID: 36738663] (Human)
• JO Williams, J Nash, C Whelan, BM Raven, AJ Davies, J Evans, L Watkeys, K Morris, PE James Early but reversible haemostatic changes in a-symptomatic females expressing COVID-19 antibodies Thrombosis Research, 2022-07-25;217(0):76-85. 2022-07-25 [PMID: 35908384] (Human)
• T Metzner, DR Leitner, G Dimsity, F Gunzer, P Opriessnig, K Mellitzer, A Beck, H Sourij, T Stojakovic, H Deutschman, W März, U Landmesser, M Brodmann, G Reishofer, H Scharnagl, H Toplak, G Silbernage Short-Term Treatment with Alirocumab, Flow-Dependent Dilatation of the Brachial Artery and Use of Magnetic Resonance Diffusion Tensor Imaging to Evaluate Vascular Structure: An Exploratory Pilot Study Biomedicines, 2022-01-11;10(1):. 2022-01-11 [PMID: 35052831] (Human)
• L Solhi, HS Sun, SH Daswani, S Shojania, CMK Springate, H Brumer Controlled sulfation of mixed-linkage glucan by Response Surface Methodology for the development of biologically applicable polysaccharides Carbohydrate polymers, 2021-06-01;269(0):118275. 2021-06-01 [PMID: 34294307] (Human)
• E Yeini, P Ofek, S Pozzi, N Albeck, D Ben-Shusha, G Tiram, S Golan, R Kleiner, R Sheinin, S Israeli Da, S Reich-Zeli, R Grossman, Z Ram, H Brem, TM Hyde, P Magod, D Friedmann-, A Madi, R Satchi-Fai P-selectin axis plays a key role in microglia immunophenotype and glioblastoma progression Nature Communications, 2021-03-26;12(1):1912. 2021-03-26 [PMID: 33771989] (Human)
• JR Shaw, V Kumar, R Mallick, M Carrier, A Ilich, NS Key, P Wells Biomarker-enhanced VTE risk stratification in ambulatory patients with cancer Thromb Res, 2020-10-08;196(0):437-443. 2020-10-08 [PMID: 33065408] (Human)
• M Mussbacher, TL Krammer, S Heber, WC Schrottmai, S Zeibig, HP Holthoff, D Pereyra, P Starlinger, M Hackl, A Assinger Impact of Anticoagulation and Sample Processing on the Quantification of Human Blood-Derived microRNA Signatures Cells, 2020-08-18;9(8):. 2020-08-18 [PMID: 32824700] (Human)
• M Z?bczyk, J Natorska, A Janion-Sad, KP Malinowski, M Janion, A Undas Elevated Lactate Levels in Acute Pulmonary Embolism Are Associated with Prothrombotic Fibrin Clot Properties: Contribution of NETs Formation J Clin Med, 2020-03-30;9(4):. 2020-03-30 [PMID: 32235490] (Human)
• G Herrera-Ma, G Vargas-Ala, O Pérez-Ménd, R Posadas-Sá, F Masso, T Juárez-Ced, G Escobedo, A Vázquez-Mo, JM Fragoso The Ser290Asn and Thr715Pro Polymorphisms of the SELP Gene Are Associated with A Lower Risk of Developing Acute Coronary Syndrome and Low Soluble P-Selectin Levels in A Mexican Population � Biomolecules, 2020-02-11;10(2):. 2020-02-11 [PMID: 32053880] (Human)
• M Dus-Zuchow, J Walkowiak, A Morawska, P Krzyzanows, A Miskiewicz, J Przyslawsk, A Lisowska Amaranth Oil Increases Total and LDL Cholesterol Levels without Influencing Early Markers of Atherosclerosis in an Overweight and Obese Population: A Randomized Double-Blind Cross-Over Study in Comparison with Rapeseed Oil Supplementation Nutrients, 2019-12-16;11(12):. 2019-12-16 [PMID: 31888234] (Human)
• N Askari, T Ghazanfari, MM Naghizadeh, A Moin, A Khamesipou, S Pourfarzam, ZM Hassan Evaluation of Association Between the Serum Levels of MMP-9 and MMP-9/TIMPs With Soluble Forms of Selectins and Itching Induced by Sulfur Mustard Iran J Pathol, 2017-01-27;12(3):257-264. 2017-01-27 [PMID: 29531551] (Human)
• N van Es, M Louzada, M Carrier, V Tagalakis, PL Gross, S Shivakumar, MA Rodger, PS Wells Predicting the risk of recurrent venous thromboembolism in patients with cancer: A prospective cohort study Thromb. Res., 2018-01-09;163(0):41-46. 2018-01-09 [PMID: 29353682] (ELISA Capture, Human)

FAQs for P-Selectin/CD62P (DPSE00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.