OXSM Antibody - BSA Free

Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.

Staining of human testis shows moderate granular cytoplasmic positivity in cells in seminiferous ducts.

Staining of human heart muscle shows moderate granular cytoplasmic positivity in cardiomyocytes.

Staining of human heart muscle, kidney, liver and testis using Anti-OXSM antibody HPA021300 (A) shows similar protein distribution across tissues to independent antibody HPA021293 (B).

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

OXSM Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: PQSEADQVATGVAIGMGMIPLEVVSETALNFQTKGYNKVSPFFVPKILVNMAAGQVSIRYKLKGPNHAVSTACTTGAHAVGDSFRFIAHGDADVMVAGGTDSCISPLSLAGF
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OXSM
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  OXSM Protein (NBP1-84732PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for OXSM Antibody - BSA Free

• 3-ketoacyl-acyl carrier protein synthase
• 3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial
• 3-oxoacyl-ACP synthase, mitochondrial
• acyl-malonyl acyl carrier protein-condensing enzyme
• beta-ketoacyl synthase
• Beta-ketoacyl-ACP synthase
• EC 2.3.1
• EC 2.3.1.41
• FASN2D
• FLJ20604
• KASI
• KS3-oxoacyl-acyl-carrier-protein synthase
• type II mitochondrial beta-ketoacyl synthase

Background

OXSM is a mitochondrial beta-ketoacyl synthase (EC 2.3.1.41) involved in mitochondrial fatty acid synthesis. It is required for catalysis of the chain-elongating condensation reaction (Zhang et al., 2005 [PubMed 15668256]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OXSM