OX40/TNFRSF4 Antibody (OX-86) - BSA Free

Flow Cytometry: OX40/TNFRSF4 Antibody (OX-86) [NB100-64847] - Staining of Concanavalin A stimulated mouse spleen cells with Rat anti Mouse

Flow Cytometry: OX40/TNFRSF4 Antibody (OX-86) [NB100-64847] - Staining of Concanavalin A stimulated mouse spleen cells with Rat anti Mouse CD134: RPE.

• Reactivity: Mu
• Applications: Flow, IHC
• Clone: OX-86
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

OX40/TNFRSF4 Antibody (OX-86) - BSA Free Summary

• Description: Novus Biologicals Rat OX40/TNFRSF4 Antibody (OX-86) - BSA Free (NB100-64847) is a monoclonal antibody validated for use in IHC and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: CHO cells transfected with murine OX40
• Localization: Cell Membrane. Type I membrane protein.
• Specificity: NB100-64847 recognises murine OX40 (CD134), a cell surface antigen expressed only by activated T lymphocytes.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: TNFRSF4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:50
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for OX40/TNFRSF4 Antibody (OX-86) - BSA Free

• ACT-135
• ACT35 antigen
• ACT35ATC35 antigen
• CD134 antigen
• CD134
• Ly-70
• OX40 cell surface antigen
• OX40 homologue
• OX40
• OX40L receptor
• OX40lymphoid activation antigene ACT35
• TAX transcriptionally-activated glycoprotein 1 receptor
• tax-transcriptionally activated glycoprotein 1 receptor
• TNFRSF4
• tumor necrosis factor receptor superfamily member 4
• tumor necrosis factor receptor superfamily, member 4
• Txgp1
• TXGP1L
• TXGP1LOX40 antigen

Background

The OX40 receptor is also known as CD134, ACT35, and tumour necrosis factor receptor superfamily member 4. This receptor is expressed on activated CD4+ and CD8+ T cells and B cells. The OX40 receptor binds to the OX40 ligand to provide a co-stimulatory signal that is independent of CD28. Blockade of OX40-OX40 ligand interactions has been shown to ameliorate experimental EAE and inflammatory bowel disease, imply that these interactions are important in the pathogenesis of some autoimmune diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for OX40/TNFRSF4 Antibody (NB100-64847). (Showing 1 - 1 of 1 FAQ).

1. What is the exact application for the NB100-64847? In your datasheet, you describe the applications are FLOW, IHC, IHC-Fr, IHC-P, but in the Uses section, you describe This antibody is useful for Immunohistochemistry-Frozen, Flow Cytometry. Which is right? And in the Recommended Dilutions section, you describe Flow Cytometry, Immunohistochemistry-Frozen, Immunohistochemistry- Paraffin, Immunohistochemistry, but there is no any information about the dilutions. What are the dilutions for this applications?
   • NB100-64847 has been tested and validated in flow cytometry and IHC-Frozen tissues. I've corrected the datasheet to reflect these changes. The recommended dilutions are 1:10-1:50 however they need to be optimized for the customers purposes.

Bioinformatics

• Gene Symbol: TNFRSF4
• Entrez:
  • 22163(Mouse)
  • 22163(Mouse)
• Uniprot:
  • P47741()
  • P47741()