OX40/TNFRSF4 Antibodies

HAF016). A specific band was detected for OX40/TNFRSF4 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF016). A specific band was detected for OX40/TNFRSF4 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human T regulatory cells (Tregs) and human peripheral blood mononuclear cells (PBMCs) untreated (-) or treated (+) with 1 µg/mL PHA for 5 days. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human OX40/TNFRSF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3388) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for OX40/TNFRSF4 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010)." class="big_lightbox"> 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010)." class="big_thumb" />

VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

1054-OX) stimulates CXCL8/IL-8 secretion in the HT1080 human fibrosarcoma cell line transfected with human OX40/TNFRSF4 in a dose-dependent manner (orange line), as measured by the Human CXCL8/IL-8 Quantikine ELISA Kit (Catalog # D8000C). CXCL8/Il-8 Secretion elicited by Recombinant Human OX40L/TNFSF4 (10 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human OX40/TNFRSF4 Polyclonal Antibody (Catalog # AF3388). The ND₅₀ is typically 0.025-0.16 µg/mL." class="big_lightbox"> 1054-OX) stimulates CXCL8/IL-8 secretion in the HT1080 human fibrosarcoma cell line transfected with human OX40/TNFRSF4 in a dose-dependent manner (orange line), as measured by the Human CXCL8/IL-8 Quantikine ELISA Kit (Catalog # D8000C). CXCL8/Il-8 Secretion elicited by Recombinant Human OX40L/TNFSF4 (10 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human OX40/TNFRSF4 Polyclonal Antibody (Catalog # AF3388). The ND₅₀ is typically 0.025-0.16 µg/mL." class="big_thumb" />

MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (F0105B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (F0105B). View our protocol for Staining Membrane-associated Proteins." alt="Human peripheral blood mononuclear cells (PBMCs) were treated with 5 μg/ml PHA for 5 days then stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Rat Anti-Human OX40/TNFRSF4 Monoclonal Antibody (Catalog # MAB3388) or (B) Rat IgG_2AIsotype Control (MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (F0105B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

F0111). Quadrant markers were set based on control antibody staining (MAB1050, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol." class="big_lightbox"> F0111). Quadrant markers were set based on control antibody staining (MAB1050, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol." alt="HEK293 human embryonic kidney cell line transfected with either (A) human OX40/TNFRSF4 or (B) irrelevant protein, and eGFP, was stained with Rabbit anti-human OX40/TNFRSF4 monoclonal antibody (Catalog # MAB10563) followed by Allophycocyanin-conjugated anti-Rabbit IgG Secondary Antibody (F0111). Quadrant markers were set based on control antibody staining (MAB1050, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol." class="big_thumb" />

1054-OX) stimulates CXCL8/IL-8 secretion in the HT1080 human fibrosarcoma cell line transfected with human OX40/TNFRSF4 in a dose-dependent manner (orange line), as measured by the Human CXCL8/IL-8 Quantikine ELISA Kit (D8000C). CXCL8/Il-8 Secretion elicited by Recombinant Human OX40L/TNFSF4 (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human OX40/TNFRSF4 Monoclonal Antibody (Catalog # MAB10563). The ND₅₀ is typically 25-250 ng/mL." class="big_lightbox"> 1054-OX) stimulates CXCL8/IL-8 secretion in the HT1080 human fibrosarcoma cell line transfected with human OX40/TNFRSF4 in a dose-dependent manner (orange line), as measured by the Human CXCL8/IL-8 Quantikine ELISA Kit (D8000C). CXCL8/Il-8 Secretion elicited by Recombinant Human OX40L/TNFSF4 (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human OX40/TNFRSF4 Monoclonal Antibody (Catalog # MAB10563). The ND₅₀ is typically 25-250 ng/mL." class="big_thumb" />

F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with (A) human OX40/TNFRSF4 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human OX40/TNFRSF4 Monoclonal Antibody (Catalog # MAB10543) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

1054-OX) stimulates CXCL8/IL-8 secretion in the HT1080 human fibrosarcoma cell line transfected with human OX40/TNFRSF4 in a dose-dependent manner (orange line), as measured by the Human CXCL8/IL-8 Quantikine ELISA Kit (Catalog # D8000C). CXCL8/Il-8 Secretion elicited by Recombinant Human OX40L/TNFSF4 (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human OX40/TNFRSF4 Monoclonal Antibody (Catalog # MAB10543). The ND₅₀ is typically 0.6-3.0 µg/mL." class="big_lightbox"> 1054-OX) stimulates CXCL8/IL-8 secretion in the HT1080 human fibrosarcoma cell line transfected with human OX40/TNFRSF4 in a dose-dependent manner (orange line), as measured by the Human CXCL8/IL-8 Quantikine ELISA Kit (Catalog # D8000C). CXCL8/Il-8 Secretion elicited by Recombinant Human OX40L/TNFSF4 (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human OX40/TNFRSF4 Monoclonal Antibody (Catalog # MAB10543). The ND₅₀ is typically 0.6-3.0 µg/mL." class="big_thumb" />

F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with (A) human OX40/TNFRSF4 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human OX40/TNFRSF4 Monoclonal Antibody (Catalog # MAB10542) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

FAB100P) and either (A) Rabbit Anti-Human OX40/TNFRSF4 Monoclonal Antibody (Catalog # MAB10545) or (B) Rabbit IgG Isotype Control (MAB1050) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody F0111). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> FAB100P) and either (A) Rabbit Anti-Human OX40/TNFRSF4 Monoclonal Antibody (Catalog # MAB10545) or (B) Rabbit IgG Isotype Control (MAB1050) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody F0111). View our protocol for Staining Membrane-associated Proteins." alt="Human peripheral blood mononuclear cells (PBMCs) were treated with 5 μg/ml PHA for 5 days then stained with Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody FAB100P) and either (A) Rabbit Anti-Human OX40/TNFRSF4 Monoclonal Antibody (Catalog # MAB10545) or (B) Rabbit IgG Isotype Control (MAB1050) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody F0111). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />