OX40/TNFRSF4 Products

HAF016). A specific band was detected for OX40/TNFRSF4 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">

5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010)." class="big_lightbox" target="_blank">

VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">

1054-OX) stimulates CXCL8/IL-8 secretion in the HT1080 human fibrosarcoma cell line transfected with human OX40/TNFRSF4 in a dose-dependent manner (orange line), as measured by the Human CXCL8/IL-8 Quantikine ELISA Kit (Catalog # D8000C). CXCL8/Il-8 Secretion elicited by Recombinant Human OX40L/TNFSF4 (10 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human OX40/TNFRSF4 Polyclonal Antibody (Catalog # AF3388). The ND₅₀ is typically 0.025-0.16 µg/mL." class="big_lightbox" target="_blank">

1054-OX) is immobilized at 1.00 μg/mL (100 μL/well), Biotinylated Recombinant Human OX40/TNFRSF4 His-tag Avi-tag (Catalog # AVI9969) binds with an ED₅₀ of 150-900 ng/mL." class="big_lightbox" target="_blank">

1054-OX) is immobilized at 250 ng/mL, the concentration of Recombinant Cynomolgus Monkey OX40/TNFRSF4 Fc Chimera (Catalog # 10311-OX) that produces the 50% optimal binding response is found to be approximately 5-30 ng/mL." class="big_lightbox" target="_blank">