Home » OX40/TNFRSF4 » OX40/TNFRSF4 Antibodies » OX40/TNFRSF4 Antibody (OX-86) [DyLight 550]

OX40/TNFRSF4 Antibody (OX-86) [DyLight 550]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, IHC-Fr
Clone
OX-86
Clonality
Monoclonal
Host
Rat
Conjugate
DyLight 550

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NB100-64847AF405
Alexa Fluor 488
NB100-64847AF488
Alexa Fluor 647
NB100-64847AF647
Alexa Fluor 700
NB100-64847AF700
Allophycocyanin
NB100-64847APC
Biotin
NB100-64847B
DyLight 350
NB100-64847UV
DyLight 405
NB100-64847V
DyLight 488
NB100-64847G
DyLight 650
NB100-64847C
DyLight 680
NB100-64847FR
DyLight 755
NB100-64847IR
FITC
NB100-64847F
HRP
NB100-64847H
PE
NB100-64847PE
PerCP
NB100-64847PCP
Unconjugated
NB100-64847
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OX40/TNFRSF4 Antibody (OX-86) [DyLight 550] Summary

Immunogen
CHO cells transfected with murine OX40
Localization
Cell Membrane. Type I membrane protein.
Specificity
NB100-64847 recognises murine OX40 (CD134), a cell surface antigen expressed only by activated T lymphocytes.
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
TNFRSF4
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for OX40/TNFRSF4 Antibody (OX-86) [DyLight 550]

  • ACT-135
  • ACT35 antigen
  • ACT35ATC35 antigen
  • CD134 antigen
  • CD134
  • Ly-70
  • OX40 cell surface antigen
  • OX40 homologue
  • OX40
  • OX40L receptor
  • OX40lymphoid activation antigene ACT35
  • TAX transcriptionally-activated glycoprotein 1 receptor
  • tax-transcriptionally activated glycoprotein 1 receptor
  • TNFRSF4
  • tumor necrosis factor receptor superfamily member 4
  • tumor necrosis factor receptor superfamily, member 4
  • Txgp1
  • TXGP1L
  • TXGP1LOX40 antigen

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Human mature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14+ cells treated with 20 ng/mL Recombinant Human IL‑4 (Catalog # 204-IL) and 50 ng/mL Recombinant Human GM‑CSF (Catalog # 215-GM) for 7 days and 1 μg/mL LPS, 20 ng/mL Recombinant Human TNF‑ alpha (Catalog # 210-TA), and 20 ng/mL Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) for last 24 hours were stained with Mouse Anti-Human OX40 Ligand/TNFSF4 Monoclonal Antibody(Catalog # MAB10541, filled histogram) or isotype control (Catalog # MAB002, open histogram), followed by Goat F(ab)2 Anti-Mouse IgG (H+L) Allophycocyanin (Catalog # F0101B).
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TNF-alpha
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1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
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Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.
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CD27/TNFRSF7 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human CD27/TNFRSF7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF382) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes in germinal center. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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[Unconjugated]
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IL-2 [Unconjugated]
202-IL
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CD8 Antibody
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NOD2 Antibody (2D9)
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OX40/TNFRSF4
Antibody (OX-86) [DyL ...
NB100-64847R
OX40/TNFRSF4 Antibody (OX-86) [DyLight 550]
Species: Mu
Applications: Flow, IHC, IHC-Fr

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NB100-64847AF405
Alexa Fluor 488 NB100-64847AF488
Alexa Fluor 647 NB100-64847AF647
Alexa Fluor 700 NB100-64847AF700
Allophycocyanin NB100-64847APC
Biotin NB100-64847B
DyLight 350 NB100-64847UV
DyLight 405 NB100-64847V
DyLight 488 NB100-64847G
DyLight 550 NB100-64847R
DyLight 650 NB100-64847C
DyLight 680 NB100-64847FR
DyLight 755 NB100-64847IR
FITC NB100-64847F
HRP NB100-64847H
PE NB100-64847PE
PerCP NB100-64847PCP
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol TNFRSF4
Entrez