OTUD5 Antibody

Western Blot: OTUD5 Antibody [NBP2-24634] - Analysis of DUBA in human liver lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse liver lysate and 4) rat liver lysate using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: OTUD5 Antibody [NBP2-24634] - Analysis of DUBA in formalin-fixed, paraffin-embedded human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.

• Reactivity: Hu, Mu, Rt, Pm, Ch, Pm, Ma-Op, RM
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

OTUD5 Antibody Summary

• Description: Novus Biologicals Rabbit OTUD5 Antibody (NBP2-24634) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A portion of amino acids 550-571 of human DUBA was used as the immunogen.
• Predicted Species: Chicken (93%), Monkey (100%), Rhesus Macaque (100%), Opossum (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OTUD5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 3-5 ug/ml

Reactivity Notes

The amino acid sequence used as immunogen is 93% homologous in Chicken, 87% homologous in Canine and 81% homologous in Equine.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for OTUD5 Antibody

• Deubiquitinating enzyme A
• DKFZp761A052
• DUBAdeubiquinating enzyme A
• EC 3.4.19.12
• MGC104871
• OTU domain containing 5
• OTU domain-containing protein 5

Background

OTUD5 encodes a member of the OTU (ovarian tumor) domain-containing cysteine protease superfamily. The OTU domain confers deubiquitinase activity and the encoded protein has been shown to suppress the type I interferon-dependent innate immune response by cleaving the polyubiquitin chain from an essential type I interferon adaptor protein. Cleavage results in disassociation of the adaptor protein from a downstream signaling complex and disruption of the type I interferon signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OTUD5