OTUD5 Antibody - BSA Free

Western Blot: OTUD5 Antibody [NBP1-76943] - Human kidney lysate with OTUD5 antibody at (A) 1 and (B) 2 ug/mL.

Immunohistochemistry: OTUD5 Antibody - BSA Free [NBP1-76943] - Immunohistochemistry of OTUD5 in mouse kidney tissue with OTUD5 antibody at 2.5 ug/mL.

Immunocytochemistry/ Immunofluorescence: OTUD5 Antibody - BSA Free [NBP1-76943] - Immunofluorescence of OTUD5 in Human Kidney cells with OTUD5 antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

OTUD5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit OTUD5 Antibody - BSA Free (NBP1-76943) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 13 amino acid synthetic peptide near the carboxy terminus of the human OTUD5. The immunogen is located within amino acids 400 - 450 of OTUD5. Amino Acid Squence: VRGPSQPKASAT
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OTUD5
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1-2 ug/ml
• Control Peptide:

  OTUD5 Peptide (NBP1-76943PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for OTUD5 Antibody - BSA Free

• Deubiquitinating enzyme A
• DKFZp761A052
• DUBAdeubiquinating enzyme A
• EC 3.4.19.12
• MGC104871
• OTU domain containing 5
• OTU domain-containing protein 5

Background

OTUD5 is a member of the OTU (ovarian tumor) domain-containing cysteine protease superfamily. The OTU domain confers deubiquitinase activity and OTUD5 has been shown to suppress the type I interferon (IFN-I)-dependent innate immune response by cleaving the polyubiquitin chain from TRAF3, an essential type I interferon adaptor protein. Cleavage results in disassociation of TRAF3 from a downstream signaling complex containing TBK1 and the disruption of the IFN-I signaling cascade, indicating that OTUD5 acts as a negative regulator of innate immune responses. It has been suggested that by suppressing IFN-I production, OTUD5 may function to inhibit the emergence of certain autoimmune disorders such as systemic lupus erythematosus. Multiple isoforms of OTUD5 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OTUD5
• Uniprot:
  • EAW50723(Human)