Otubain-2 Antibody - BSA Free

Immunohistochemistry-Paraffin: Otubain-2 Antibody [NBP1-88410] - Staining in human testis and skeletal muscle tissues using anti-OTUB2 antibody. Corresponding OTUB2 RNA-seq data are presented for the same tissues.

Western Blot: Otubain-2 Antibody [NBP1-88410] - Analysis in control (vector only transfected HEK293T lysate) and OTUB2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry-Paraffin: Otubain-2 Antibody [NBP1-88410] - Staining of human testis shows distinct nuclear positivity in seminiferus duct cells.

Immunohistochemistry-Paraffin: Otubain-2 Antibody [NBP1-88410] - Staining of human skeletal muscle shows low expression as expected.

Analysis in control (vector only transfected HEK293T lysate) and OTUB2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Otubain-2 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: YSVVELVEKDGSVSSLLKVFNDQSASDHIVQFLRLLTSAFIRNRADFFRHFIDEEMDIKDFCTHEVEPMATECDHIQITALSQALSIALQVEYVDEMDTALNHHVFPEAATPSVYLLYKTSHYNIL
• Predicted Species: Mouse (94%), Rat (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OTUB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Otubain-2 Recombinant Protein Antigen (NBP1-88410PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Otubain-2 Antibody - BSA Free

• C14orf137
• C14orf137chromosome 14 open reading frame 137
• Deubiquitinating enzyme OTUB2
• EC 3.4.19.12
• FLJ21916
• MGC3102
• OTB2otubain-2
• OTU domain, ubiquitin aldehyde binding 2
• OTU domain-containing ubiquitin aldehyde-binding protein 2
• OTU2
• OTU2ubiquitin thioesterase OTUB2
• OTUB2
• Otubain2
• Otubain-2
• ubiquitin-specific protease otubain 2
• Ubiquitin-specific-processing protease OTUB2

Background

Otubains are deubiquitylating cysteine proteases (DUBs; see MIM 602519) that belong to the ovarian tumor (OTU) protein superfamily. Like other DUBs, otubains cleave proteins precisely at the ubiquitin (UB; see MIM 191339)-protein bond.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OTUB2