Otubain-1 Antibody

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Western Blot: Otubain-1 Antibody [NBP1-49934] - Detection of Human and Mouse OTUB1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

Otubain-1 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 1 and 40 of human OTU Domain, Ubiquitin Aldehyde Binding 1 using the numbering given in entry NP_060140.2 (GeneID 55611).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OTUB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 2-5 ug/mg lysate

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Otubain-1 Antibody

  • Deubiquitinating enzyme OTUB1
  • EC 3.1.2
  • EC 3.4.19.12
  • FLJ20113
  • FLJ40710
  • hOTU1
  • MGC111158
  • OTB1
  • OTB1MGC4584
  • OTU domain, ubiquitin aldehyde binding 1
  • OTU domain-containing ubiquitin aldehyde-binding protein 1
  • OTU1
  • OTU1OTU-domain Ubal-binding 1
  • OTUB1
  • Otubain1
  • Otubain-1
  • ubiquitin thioesterase OTUB1
  • ubiquitin-specific protease otubain 1
  • Ubiquitin-specific-processing protease OTUB1

Background

The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol OTUB1
Entrez