![Immunocytochemistry/Immunofluorescence: Otubain-2 Antibody (139) [NBP2-90191] - Immunofluorescence staining of Human Otubain-2 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90191 antibody (1:60) at 4C overnight. Then cells were stained with the Alexa Fluor(TM) 549-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-90191_rabbit-monoclonal-otubain-2-antibody-139-23520251664756.png "Immunocytochemistry/Immunofluorescence: Otubain-2 Antibody (139) [NBP2-90191] - Immunofluorescence staining of Human Otubain-2 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90191 antibody (1:60) at 4C overnight. Then cells were stained with the Alexa Fluor(TM) 549-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm and nucleus.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, ICC/IF |
| Clone | 139 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | This antibody was obtained from a rabbit immunized with purified, recombinant Human Otubain-2 (Uniprot#: Q96DC9-1; Met1-His234). |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | OTUB2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | OTUB2 |
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